Enhanced production of recombinant nattokinase in Bacillus subtilis by the elimination of limiting factors

Biotechnology Letters

Volumn 28, Issue 19, 2006, Pages 1595-1600

  Chen, Po Ting ^a   Chao, Yun Peng ^a  

^a FENG CHIA UNIVERSITY   (Taiwan)

Author keywords

Bacillus subtilis; Nattokinase; Recombinant protein production

Indexed keywords

BACILLUS SUBTILIS; METAL IONS; NATTOKINASE; RECOMBINANT PROTEIN PRODUCTION; UNSUPPLEMENTED MEDIUM;

BACTERIA; BIOLOGICAL MATERIALS; GROWTH KINETICS;

ENZYME KINETICS;

NATTOKINASE; RECOMBINANT PROTEIN; SUBTILISIN;

ARTICLE; BACILLUS SUBTILIS; BIOLOGICAL MODEL; BIOSYNTHESIS; CELL CULTURE; GENE VECTOR; GENETICS; METABOLISM; PLASMID; UPREGULATION;

BACILLUS SUBTILIS; CELLS, CULTURED; GENETIC VECTORS; MODELS, BIOLOGICAL; PLASMIDS; RECOMBINANT PROTEINS; SUBTILISINS; UP-REGULATION;

BACILLUS SUBTILIS;

EID: 33748093547     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9126-3     Document Type: Article

References (16)

1. Artemov AV, Samuilov VD (1990) Effect of polyelectrolytes on serine proteinase secretion by Bacillus subtilis. FEBS Lett 262:33-35
2. Chao Y-P, Chiang CJ, Hung WB (2002) Stringent regulation and high-level expression of heterologous genes in Escherichia coli using T7 expression system controllable by the araBAD promoter. Biotechnol Prog 18:394-400
3. Gill RT, Valdes JJ, Bentley WE (2000) A comparative study of global stress gene regulation in response to overexpression of recombinant proteins in Escherichia coli. Metabolic Eng 2:178-189
4. Hardwood CR Cutting SM (eds) (1990) Molecular biological methods for Bacillus. John Wiley & Sons, England
5. Hu H-B, Yao S-J, Mei L-H, Zhu Z-Q, Hur B-K. (2000) Partial purification of nattokinase from Bacillus subtilis by expanded bed adsorption. Biotechnol Lett 23:1383-1387
6. Kurland GC, Dong H (1996) Bacterial growth inhibition by overproduction of protein. Mol Microbiol 21:1-4
7. Li W, Zhou X, Lu P (2004) Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis. Res Microbiol 155:605-610
8. Nakamura T, Yamagata Y, Ichishima E (1992) Nucleotide sequence of the subtilisin NAT gene, aprN, of Bacillus subtilis (natto). Biosci Biotech Biochem 56:1869-1871
9. Ramirez DM, Bentley WE (1993) Enhancement of recombinant protein synthesis and stability via coordinated amino acid addition. Biotechnol Bioeng 41:557-565
10. Schreier HJ (1993) Biosynthesis of glutamine and glutamate and the assimilation of ammonia. In: Sonenshein AL, Hoch JA, Losick R (eds) Bacillus subtilis and other Gram-positive bacteria, biochemistry, physiology, and molecular genetics. American Society for Microbiology, Washington, DC
11. Urano T, Ihara H, Umemura K, Suzuki Y, Oike M, Akita S, Tsukamoto Y, Suzuki I, Takada A (2001) The profibrinolytic enzyme subtilisin NAT purified from Bacillus subtilis cleaves and inactivates plasminogen activator inhibitor type I. J Biol Chem 276:24690-24696
12. 37 RNA polymerase holoenzymes during growth and stationary phases. J Biol Chem 259:8619-8625
13. Wang ZW, Chen Y, Chao Y-P (2006) Enhancement of recombinant protein production in Escherichia coli by coproduction of aspartase. J Biotech (in press)
14. Westers L, Westers H, Quax WJ (2004) Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism. Biochim Biophys Acta 1694:299-310
15. Wu S-C, Wong S-L (1999) Development of improved pUB110-based vectors for expression and secretion studies in Bacillus subtilis. J Biotech 72:185-195
16. Xiao L, Zhang R-H, Peng Y, Zhang Y-Z (2004) Highly efficient gene expression of a fibrinolytic enzyme (subtilisin DFE) in Bacillus sutilis mediated by the promoter of a-amylase gene form Bacillus amyloliquefacines. Biotechnol Lett 26:1365-1369