Biosynthesis of proteins containing modified lysines and fluorescent labels using non-natural amino acid mutagenesis

Journal of Bioscience and Bioengineering

Volumn 111, Issue 4, 2011, Pages 402-407

  Tokuda, Yasunori ^a   Watanabe, Takayoshi ^a   Horiike, Kazushi ^a   Shiraga, Kaori ^a   Abe, Ryoji ^a   Muranaka, Norihito ^a   Hohsaka, Takahiro ^a  

^a JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

Author keywords

Cell free translation; Fluorescence labeling; Non natural amino acid; Posttranslational modification; Protein engineering

Indexed keywords

CELL-FREE; FLUORESCENCE LABELING; NON NATURAL AMINO ACIDS; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN ENGINEERING;

AMINO ACIDS; BINDING SITES; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; GENETIC ENGINEERING; MUTAGENESIS;

CALMODULIN;

AMINO ACID; CALMODULIN; DIMETHYLLYSINE; FLUORESCENT DYE; HISTONE H3; LYSINE; METHYLLYSINE; N ACETYLLYSINE; STREPTAVIDIN; TRANSFER RNA; TRIMETHYLLYSINE; UNCLASSIFIED DRUG;

AMINOACYLATION; ARTICLE; CELL FREE SYSTEM; ESCHERICHIA COLI; FLUORESCENCE CORRELATION SPECTROSCOPY; IMMUNODETECTION; MUTAGENESIS; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN SYNTHESIS;

AMINO ACIDS; AMINOACYLATION; CELL-FREE SYSTEM; ESCHERICHIA COLI; FLUORESCENT DYES; LYSINE; MUTAGENESIS; PROTEIN BIOSYNTHESIS; PROTEIN ENGINEERING; PROTEINS; RNA, TRANSFER; STREPTAVIDIN;

ESCHERICHIA COLI;

EID: 79953316179     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2010.12.012     Document Type: Article

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