Purification of ribosomes from human cell lines

Current Protocols in Cell Biology

Volumn , Issue SUPPL.49, 2010, Pages

  Belin, Stéphane ^a,b   Hacot, Sabine ^a   Daudignon, Lionel ^a   Therizols, Gabriel ^a   Pourpe, Stéphane ^a   Mertani, Hichem C ^a   Rosa Calatrava, Manuel ^a   Diaz, Jean Jacques ^a  

^a UNIVERSITÉ DE LYON   (France)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Cell fractionation; Ribosome; Translation

Indexed keywords

NUCLEOLIN; RIBOSOME PROTEIN; RIBOSOME RNA; RIBOZYME;

ARTICLE; BIOGENESIS; CRYSTALLOGRAPHY; EUKARYOTIC CELL; HUMAN; HUMAN CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; RIBOSOME;

EUKARYOTA; MAMMALIA;

EID: 79953187584     PISSN: 19342500     EISSN: 19342616     Source Type: Journal    
DOI: 10.1002/0471143030.cb0340s49     Document Type: Article

References (48)

1. Balis, M.E., Samarth, K.D., Hamilton, M.G., and Petermann, M.L. 1958. Role of the ribonucleoprotein particle in protein synthesis and the effects of growth hormone. J. Biol. Chem. 233:1152-1155.
2. Ban, N., Nissen, P., Hansen, J.H., Moore, P.B., and Steitz, T.A. 2000. The complete atomic structure of the large ribosomal subunit at 2.4 Å resolution. Science 289:905-920.
3. Belin, S., Beghin, A., Solano-Gonzalez, E., Bezin, L., Brunet-Manquat, S., Textoris, J., Prats, A.C., Mertani, H.C., Dumontet, C., and Diaz, J.J. 2009. Dysregulationof ribosome biogenesis and translational capacity is associated with tumor progression of human breast cancer cells. PLoS ONE 4:e7147.
4. Belin, S., Kindbeiter, K., Hacot, S., Albaret, M.A., Roca-Martinez, J.X., Therizols, G., Grosso, R., and Diaz, J.J. 2010. Uncoupling ribosome biogenesis regulation from RNA polymerase I activity during herpes simplex virus type 1 infection. RNA 16:131-140.
5. Cech, T.R. 2000. Structural biology. The ribosome is a ribozyme. Science 289:878-879.
6. Chan, Y.L., Diaz, J.J., Denoroy, L., Madjar, J.J., and Wool, I.G. 1996. The primary structure of rat ribosomal protein L10: Relationship to a Jun-binding protein and to a putative Wilms' tumor suppressor. Biochem. Biophys. Res. Commun. 225:952-956.
7. Chandramouli, P., Toof, M., Menetret, J.F., Eswar, N., Cannone, J.J., Gutell, R.R., Sali,. A., and Akev, C.W. 2008. Structure of the mammalian 80S ribosome at 8.7 Å resolution. Structure 16:535-548.
8. Couté, Y., Burgess, J.A., Diaz, J.J., Chichester, C., Lisacek, F., Greco, A., and Sanchez, J.C. 2006. Deciphering the human nucleolar proteome. Mass Spectrom. Rev. 25:215-234.
9. Decatur, W.A. and Fournier, M.J. 2002. rRNA modifications and ribosome function. Trends Biochem. Sci. 27:344-351.
10. Diaz, J.J., Gandrillon, O., Hentzen, D., Leguellic, D., Samarut, J., and Madjar, J.J. 1989. Increase in ribosomal protein S6 phosphorylation is due to verbB-transforming activity and not to verbA mitogenic activity in avian erythroblastosis virus-infected chicken embryo fibroblasts. Oncogene Res. 4:163-175.
11. Diaz, J.J., Simonin, D., Masse, T., Deviller, P., Kindbeiter, K., Denoroy, L., and Madjar, J.J. 1993. The herpes simplex virus type 1 US11 gene product is a phosphorylated protein found to be non-specifically associated with both ribosomal subunits. J. Gen. Virol. 74:397-406.
12. Diaz, J.J., Dodon, M.D., Schaerer-Ulthurralt, N., Simonin, D., Kindbeiter, K., Gazzolo, L., and Madjar, J.J. 1996. Post-transcriptional transactivation of human retroviral envelope glycoprotein expression by herpes simplex virus Us11 protein. Nature 379:273-277.
13. Diaz J.J., Giraud, S., and Greco, A. 2002. Alteration of ribosomal protein maps in herpes simplex virus type 1 infection. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 771:237-249.
14. Fromont-Racine, N.M., Sanger, B., Saveanu, C., and Pasiolo, F. 2003. Ribosome assembly in eukaryotes. Gene 313:17-42.
15. Gallagher, S.R. 2006. One-dimensional SDS gel electrophoresis of proteins. Curr. Protoc. Mol. Biol. 75:10.2A.1-10.2A.37.
16. Görg, A., Obermaier, C., Boguth, G., Csordas, A., Diaz, J.J., and Madjar, J.J. 1997. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis 18:328-337.
17. Greco, A., Bienvenut, W., Sanchez, J.C., Kindbeiter, K., Hochstrasser, D., Madjar, J.J., and Diaz, J.J. 2001. Identification of ribosome-associated viral and cellular basic proteins during the course of infection with herpes simplex virus type 1. Proteomics 1:545-549.
18. Idol, R.A., Robledo. S., Du, H.Y., Crimmins, D.L., Wilson, D.B., Ladenson, J.H., Besler, M., and Mason, P.J. 2007. Cells depleted for RPS19, a protein associated with Diamond Blackfan Anemia, show defects in 18S ribosomal RNA synthesis and small ribosomal subunit production. Blood Cells Mol. Dis. 39:35-43.
19. Komili, S., Farny, N.G., Roth, F.P., and Silver, P.A. 2007. Functional specificity among ribosomal proteins regulates gene expression. Cell 131:557-571.
20. Madjar, J.J. 1994. Preparation of ribosomes and ribosomal proteins from cultured cells. In Cell Biology: A Laboratory Handbook (J.E. Celis, ed.) pp. 657-661. Academic Press, New York.
21. Madjar, J.J. and Fournier, A. 1981. Bombyx mori L. ribosomal proteins: Resolution, nomenclature, molecular weights and in vivo phosphorylation. Mol. Gen. Gene. 182:273-278.
22. Madjar, J.J., Arpin, M., Marion, M.J., and Reboud, J.P. 1977. Comparison of the protein content of free and membrane-bound rat liver polysomes and of the derived subunits. Mol. Biol. Rep. 3:289-296.
23. Madjar, J.J., Arpin, M., Buisson, M., and Reboud, P. 1979. Spot position of rat liver ribosomal proteins by four different two-dimensional electrophoreses in polyacrylamide gel. Mol. Gen. Genet. 171:121-134.
24. Makowski, I., Frolow, F., Saper, M.A., Shoham, M., Whitmann, H.G., and Yonath, A. 1987. Single crystals of large ribosomal particles from Halobacterium marismortui diffract to 6 Å. J. Mol. Biol. 193:819-822.
25. Massé, T., Garcin, D., Jacquemont, B., and Madjar, J.J. 1990a. Herpes simplex virus type-1-induced stimulation of ribosomal protein S6 phosphorylation is inhibited in neomycin-treated human epidermoid carcinoma 2 cells and in rastransformed cells. Eur. J. Biochem. 194:287-291.
26. Massé, T., Garcin, D., Jacquemont, B., and Nadjar, J.J. 1990b. Ribosome and protein synthesis modifications after infection of human epidermoid carcinoma cells with herpes simplex virus type 1. Mol. Gen. Genet. 220:377-388.
27. Moore, P.B. and Steitz, T.A. 2002. The involvement of RNA in ribosome function. Nature 418:229-235.
28. Narla, A. and Ebert, B.L. 2010. Ribosomopathies: Human disorders of ribosome dysfunction. Blood 115:3196-3205.
29. Nissen, P., Hansen, J., Ban, N., Moore, P.B., and Steitz, T.A. 2000. The structural basis of ribosome activity in peptide bond synthesis. Science 289:920-930.
30. Palade, G.E. 1955. A small particulate component of the cytoplasm. J. Biophys. Biochem. Cytol. 1:59-68.
31. Rudra, D. and Warner, J.R.. 2004. What better measure than ribosome synthesis? Genes Dev. 18: 2431-2436.
32. Ruggero, D. and Pandolfi, P.P. 2003. Does the ribosome translate cancer? Nat. Rev. Cancer 3:179-192.
33. Sasse, J. and Gallagher, S.R. 2009. Staining proteins in gels. Curr. Protoc. Mol. Biol. 85:10.6.1-10.6.27.
34. Schuwirth, B.S., Borovinskaya, M.A., Hau, C.W., Zhang, W., Vila-Sanjurjo, A., Holton, J.M., and Cate, J.H. 2005. Structures of the bacterial ribosome at 3.5 Å resolution. Science 310:827-834.
35. Simonin, M.H. and Smith, J.A. 2008. Spectrophotometric and colorimetric determination of protein concentration. Curr. Protoc. Mol. Biol. 76:10.1A.1-10.1A.9.
36. Simonin, D., Diaz, J.J., Kindbeiter, K., Pernas, P., and Madjar, J.J. 1995a. Phosphorylation of herpes simplex virus type 1 Us11 protein is independent of viral genome expression. Electrophoresis 16:1317-1322.
37. Simonin, D., Diaz, J.J., Kindbeiter, K., Denory, L., and Madjar, J.J. 1995b. Phosphorylation of ribosomal protein L30 after herpes simplex virus type 1 infection. Electrophoresis 16:854-859.
38. Simonin, D., Diaz, J.J., Masse, T., and Madjar, J.J. 1997. Persistence of ribosomal protein synthesis after infection of HeLa cells by herpes simplex virus type 1. J. Gen. Virol. 78:435-443.
39. Spahn, C.M., Beckmann, R., Eswar, N., Penczek, P.A., Sali, A., Blobel, G., and Frank, J. 2001. Structure of the 80S ribosome from Saccharomyces cerevisiae-tRNA-ribosome and subunit-subunit interactions. Cell 107:373-386.
40. Tissieres, A. and Watson, J.D. 1958. Ribonucleo-protein particles from Escherichia coli. Nature 182:778-780.
41. Trakhanov, S., Yusupov, M., Shirokov, V., Garber, M., Mitschlker, A., Ruff, M., Thiery, J.C., and Moras, D. 1989. Preliminary X-ray investigation of 70S ribosome crystals from Thermus thermophilus. J. Mol. Biol. 209:327-328.
42. Warner, J.R. 1999 The economics of ribosome biosynthesis in yeast. Trends Biochem. Sci. 24:437-440.
43. Warner, J.R. and Knopf, P.M. 2002. The discovery of polyribosomes. Trends Biochem. Sci. 27:376-380.
44. Wimberly, B.T., Brodersen, D.E., Clemons, W.M. Jr., Morgan-Warren, R.J., Carter, A.P., Vonrhein, C., Hartsch, T., and Ramakrishnan, V. 2000. Structure of the 30S ribosomal subunit. Nature 407:327-339.
45. Wool, I.G., Chan, Y.-L., and Glück, A. 1996. Mammalian ribosomes: the structure and the evolution of the proteins. In Translational Control (J.W.B. Hershey and M.B. Mathews, eds.) pp. 685-732. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
46. Yonath, A., Bartunik, H.D., Bartels, K.S., and Witmann, H.G. 1984. Some x-ray diffraction patterns from single crystals of the large ribosomal subunit from Bacillus stearothermophilus. J. Mol. Biol. 177:201-206.
47. Yoon, A., Peng, G., Brandenburger, Y., Zoillo, O., Xu, W., Rego, E., and Ruggero, D. 2006. Impaired control of IRES-mediated translation in X-linked dyskeratosis congenita. Science 312:902-906.
48. Yusupov, M.M., Yusupova, G.Z., Baucom, A., Lieberman, K., Earnest, T.N., Cate, J.H., and Noller, H.F. 2001. Crystal structure of the ribosome at 5.5 Å resolution. Science 292:883-896.