메뉴 건너뛰기




Volumn 680, Issue , 2011, Pages 77-88

Quantification of miRNA Abundance in Single Cells Using Locked Nucleic Acid-FISH and Enzyme-Labeled Fluorescence

Author keywords

enzyme labeled fluorescence (ELF); fluorescence in situ hybridization (FISH); locked nucleic acid (LNA); miRNA; single molecule detection

Indexed keywords

MICRORNA; NUCLEIC ACID;

EID: 79953153834     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-901-7_5     Document Type: Chapter
Times cited : (13)

References (19)
  • 1
    • 11844278458 scopus 로고    scopus 로고
    • Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets
    • Lewis, B. P., Burge, C. B., and Bartel, D. P. (2005) Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell 120, 15–20.
    • (2005) Cell , vol.120 , pp. 15-20
    • Lewis, B.P.1    Burge, C.B.2    Bartel, D.P.3
  • 2
    • 18344369543 scopus 로고    scopus 로고
    • MicroRNA biogenesis: Coordinated cropping and dicing
    • Kim, V. N. (2005) MicroRNA biogenesis: coordinated cropping and dicing. Nat. Rev. Mol. Cell Biol. 6, 376–85.
    • (2005) Nat. Rev. Mol. Cell Biol. , vol.6 , pp. 376-385
    • Kim, V.N.1
  • 3
    • 0347755643 scopus 로고    scopus 로고
    • The microRNA Registry
    • Griffiths-Jones, S. (2004) The microRNA Registry. Nucleic Acids Res. 32, D109–11.
    • (2004) Nucleic Acids Res , vol.32 , pp. D109-D111
    • Griffiths-Jones, S.1
  • 4
    • 35348821616 scopus 로고    scopus 로고
    • MicroRNAs in vertebrate physiology and human disease
    • Chang, T. C. and Mendell, J. T. (2007) MicroRNAs in vertebrate physiology and human disease. Annu. Rev. Genomics Hum. Genet. 8, 215–39.
    • (2007) Annu. Rev. Genomics Hum. Genet. , vol.8 , pp. 215-239
    • Chang, T.C.1    Mendell, J.T.2
  • 5
    • 0034307684 scopus 로고    scopus 로고
    • Probability in transcriptional regulation and its implications for leukocyte differentiation and inducible gene expression
    • Hume, D. A. (2000) Probability in transcriptional regulation and its implications for leukocyte differentiation and inducible gene expression. Blood 96, 2323–8.
    • (2000) Blood , vol.96 , pp. 2323-2328
    • Hume, D.A.1
  • 6
    • 0035193294 scopus 로고    scopus 로고
    • Stochasticity in transcriptional regulation: Origins, consequences, and mathematical representations
    • Kepler, T. B. and Elston, T. C. (2001) Stochasticity in transcriptional regulation: origins, consequences, and mathematical representations. Biophys. J. 81, 3116–36.
    • (2001) Biophys. J. , vol.81 , pp. 3116-3136
    • Kepler, T.B.1    Elston, T.C.2
  • 7
    • 0028328719 scopus 로고
    • Transcription of individual genes in eukaryotic cells occurs randomly and infrequently
    • Ross, I. L., Browne, C. M., and Hume, D. A. (1994) Transcription of individual genes in eukaryotic cells occurs randomly and infrequently. Immunol. Cell Biol. 72, 177–85.
    • (1994) Immunol. Cell Biol. , vol.72 , pp. 177-185
    • Ross, I.L.1    Browne, C.M.2    Hume, D.A.3
  • 8
    • 0036790975 scopus 로고    scopus 로고
    • Intrinsic and extrinsic contributions to stochasticity in gene expression
    • Swain, P. S., Elowitz, M. B., and Siggia, E. D. (2002) Intrinsic and extrinsic contributions to stochasticity in gene expression. Proc. Natl. Acad. Sci. USA 99, 12795–800.
    • (2002) Proc. Natl. Acad. Sci. USA , vol.99 , pp. 12795-12800
    • Swain, P.S.1    Elowitz, M.B.2    Siggia, E.D.3
  • 9
    • 31344454565 scopus 로고    scopus 로고
    • In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes
    • Kloosterman, W. P., Wienholds, E., de Bruijn, E., Kauppinen, S., and Plasterk, R. H. (2006) In situ detection of miRNAs in animal embryos using LNA-modified oligonucleotide probes. Nat. Methods 3, 27–29.
    • (2006) Nat. Methods , vol.3 , pp. 27-29
    • Kloosterman, W.P.1    Wienholds, E.2    de Bruijn, E.3    Kauppinen, S.4    Plasterk, R.H.5
  • 10
    • 31044455031 scopus 로고    scopus 로고
    • RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain
    • Nelson, P. T., Baldwin, D. A., Kloosterman, W. P., Kauppinen, S., Plasterk, R. H., and Mourelatos, Z. (2005) RAKE and LNA-ISH reveal microRNA expression and localization in archival human brain. RNA. 12, 187–91.
    • (2005) RNA , vol.12 , pp. 187-191
    • Nelson, P.T.1    Baldwin, D.A.2    Kloosterman, W.P.3    Kauppinen, S.4    Plasterk, R.H.5    Mourelatos, Z.6
  • 11
    • 35548971658 scopus 로고    scopus 로고
    • MicroRNA-206 colocal-izes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myo-genic cells
    • Politz, J. C., Zhang, F., and Peder-son, T. (2006) MicroRNA-206 colocal-izes with ribosome-rich regions in both the nucleolus and cytoplasm of rat myo-genic cells. Proc. Natl. Acad. Sci. USA 103, 18957–62.
    • (2006) Proc. Natl. Acad. Sci. USA , vol.103 , pp. 18957-18962
    • Politz, J.C.1    Zhang, F.2    Peder-Son, T.3
  • 12
    • 38449091863 scopus 로고    scopus 로고
    • Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyra-mide signal amplification
    • Silahtaroglu, A. N., Nolting, D., Dyrskjot, L., Berezikov, E., Moller, M., Tommerup, N., and Kauppinen, S. (2007) Detection of microRNAs in frozen tissue sections by fluorescence in situ hybridization using locked nucleic acid probes and tyra-mide signal amplification. Nat. Protoc. 2, 2520–8.
    • (2007) Nat. Protoc. , vol.2 , pp. 2520-2528
    • Silahtaroglu, A.N.1    Nolting, D.2    Dyrskjot, L.3    Berezikov, E.4    Moller, M.5    Tommerup, N.6    Kauppinen, S.7
  • 14
    • 33644876042 scopus 로고    scopus 로고
    • Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis
    • Chou, L. S., Meadows, C., Wittwer, C. T., and Lyon, E. (2005) Unlabeled oligonucleotide probes modified with locked nucleic acids for improved mismatch discrimination in genotyping by melting analysis. Biotechniques 39, 644, 646, 648 passim.
    • (2005) Biotechniques , vol.39 , pp. 644
    • Chou, L.S.1    Meadows, C.2    Wittwer, C.T.3    Lyon, E.4
  • 15
    • 2342520656 scopus 로고    scopus 로고
    • Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) geno-type analysis and validation using real-time PCR
    • Johnson, M. P., Haupt, L. M., and Griffiths, L. R. (2004) Locked nucleic acid (LNA) single nucleotide polymorphism (SNP) geno-type analysis and validation using real-time PCR. Nucleic Acids Res. 32, e55.
    • (2004) Nucleic Acids Res , vol.32 , pp. e55
    • Johnson, M.P.1    Haupt, L.M.2    Griffiths, L.R.3
  • 16
    • 16544365954 scopus 로고    scopus 로고
    • Sensitive and specific detection of microR-NAs by northern blot analysis using LNA-modified oligonucleotide probes
    • Valoczi, A., Hornyik, C., Varga, N., Burgyan, J., Kauppinen, S., and Havelda, Z. (2004) Sensitive and specific detection of microR-NAs by northern blot analysis using LNA-modified oligonucleotide probes. Nucleic Acids Res. 32, e175.
    • (2004) Nucleic Acids Res , vol.32 , pp. e175
    • Valoczi, A.1    Hornyik, C.2    Varga, N.3    Burgyan, J.4    Kauppinen, S.5    Havelda, Z.6
  • 17
    • 33646849284 scopus 로고    scopus 로고
    • Design of LNA probes that improve mismatch discrimination
    • You, Y., Moreira, B. G., Behlke, M. A., and Owczarzy, R. (2006) Design of LNA probes that improve mismatch discrimination. Nucleic Acids Res. 34, e60.
    • (2006) Nucleic Acids Res , vol.34 , pp. e60
    • You, Y.1    Moreira, B.G.2    Behlke, M.A.3    Owczarzy, R.4
  • 18
    • 69049086730 scopus 로고    scopus 로고
    • Imaging individual microRNAs in single mammalian cells in situ
    • Lu, J. and Tsourkas, A. (2009) Imaging individual microRNAs in single mammalian cells in situ. Nucleic Acids Res. 37, e100.
    • (2009) Nucleic Acids Res , vol.37 , pp. e100
    • Lu, J.1    Tsourkas, A.2
  • 19
    • 0030899391 scopus 로고    scopus 로고
    • The ELF-97 alkaline phosphatase substrate provides a bright, photostable, fluorescent signal amplification method for FISH
    • Paragas, V. B., Zhang, Y. Z., Haugland, R. P., and Singer, V. L. (1997) The ELF-97 alkaline phosphatase substrate provides a bright, photostable, fluorescent signal amplification method for FISH. J. Histochem. Cytochem. 45, 345–57.
    • (1997) J. Histochem. Cytochem. , vol.45 , pp. 345-357
    • Paragas, V.B.1    Zhang, Y.Z.2    Haugland, R.P.3    Singer, V.L.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.