Application of DBS for quantitative assessment of the peptide Exendin-4; Comparison of plasma and DBS method by UHPLC-MS/MS

Bioanalysis

Volumn 2, Issue 8, 2010, Pages 1461-1468

  Kehler, Jonathan R ^a   Bowen, Chester L ^a   Boram, Sharon L ^a   Evans, Christopher A ^a  

^a GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EXENDIN 4; PEPTIDE; VENOM;

ACCURACY; ARTICLE; BLOOD SAMPLING; CALIBRATION; CONTROLLED STUDY; DRIED BLOOD SPOT; DRUG DETERMINATION; DRUG STABILITY; HAPLORHINI; INTERMETHOD COMPARISON; LIMIT OF QUANTITATION; NONHUMAN; QUANTITATIVE ANALYSIS; REPRODUCIBILITY; ROOM TEMPERATURE; SENSITIVITY AND SPECIFICITY; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; VALIDATION STUDY; AMINO ACID SEQUENCE; ANIMAL; BLOOD; BLOOD ANALYSIS; CHEMISTRY; COMPARATIVE STUDY; DESICCATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METHODOLOGY; MOLECULAR GENETICS; PLASMA; TIME;

AMINO ACID SEQUENCE; ANIMALS; BLOOD CHEMICAL ANALYSIS; BLOOD SPECIMEN COLLECTION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DESICCATION; HAPLORHINI; MOLECULAR SEQUENCE DATA; PEPTIDES; PLASMA; TANDEM MASS SPECTROMETRY; TIME FACTORS; VENOMS;

EID: 79953146972     PISSN: 17576180     EISSN: 17576199     Source Type: Journal    
DOI: 10.4155/bio.10.108     Document Type: Article

References (7)

1. Barfeld M, Spooner N, Lad R, Parry S, Fowles S. Application of dried blood spots combined with HPLC-MS/MS for the quantifcation of acetaminophen in toxicokinetic studies. J. Chromatogr. B 870, 32-37 (2008).
2. Spooner N, Lad R, Barfeld M. Dried blood spots as a sample collection technique for the determination of pharmacokinetics in clinical studies: considerations for the validation of a quantitative bioanalytical method. Anal. Chem. 81, 1557-1563 (2009).
3. Li W, Tse FLS. Dried blood spot sampling in combination with LC-MS/MS for quantitative ana lysis of small molecules. Biomed. Chromatogr. 24, 49-65 (2010).
4. Viswanathan CT, Bansal S, Booth B et al. Quantitative bioanalytical methods validation and implementation: best practices for chromatographic and ligand binding assays. Pharm. Res. 24, 1962-1973 (2007). (Pubitemid 47389236)
5. Lee JC, Timashef SN. The stabilization of proteins by sucrose. Biol. Chem. 256, 7190-7201 (1981).
6. Abu-Rabie P, Spooner N. Direct quantitative Bioanalysis of drugs in dried blood spot samples using a thin-layer chromatography mass spectrometer interface. Anal. Chem. 81, 10275-10284 (2009).
7. Wiseman JM, Evans CA, Bowen CL, Kennedy JH. Direct ana lysis of dried blood spots utilizing desorption electrospray ionization (DESI) mass spectrometry. Analyst 135, 720-725 (2010).