Use of serum free light chain analysis and urine protein electrophoresis for detection of monoclonal gammopathies

Clinical Chemistry and Laboratory Medicine

Volumn 49, Issue 1, 2011, Pages 83-88

  Holding, Stephen ^a,b   Spradbery, Dorothy ^a   Hoole, Robin ^a   Wilmot, Rachel ^a   Shields, Michael L ^c   Levoguer, Alison M ^d   Doré, Philip C ^a  

^a HULL ROYAL INFIRMARY   (United Kingdom)

^b UNIVERSITY OF HULL   (United Kingdom)

^c ROYAL SHREWSBURY HOSPITAL   (United Kingdom)

^d Binding Site Group Ltd   (United Kingdom)

Author keywords

Bence Jones protein; Monoclonal gammopathy; Paraproteinaemia; Serum free light chain; Serum protein electrophoresis

Indexed keywords

BENCE JONES PROTEIN;

ARTICLE; CLINICAL ASSESSMENT; DIAGNOSTIC ACCURACY; DIAGNOSTIC TEST ACCURACY STUDY; HUMAN; LIGHT CHAIN; MAJOR CLINICAL STUDY; MONOCLONAL IMMUNOGLOBULINEMIA; PRIORITY JOURNAL; PROTEIN ELECTROPHORESIS; PROTEIN URINE LEVEL; SENSITIVITY ANALYSIS; URINALYSIS;

BENCE JONES PROTEIN; COHORT STUDIES; ELECTROPHORESIS; FEMALE; HUMANS; IMMUNOGLOBULIN LIGHT CHAINS; MALE; PARAPROTEINEMIAS; PROTEINURIA; RETROSPECTIVE STUDIES; URINALYSIS;

EID: 79953129617     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2011.010     Document Type: Article

References (33)

1. Abraham RS, Katzmann JA, Clark RJ, Bradwell AR, Kyle RA, Gertz MA. Quantitative analysis of serum free light chains. A new marker for the diagnostic evaluation of primary systemic amyloidosis. Am J Clin Pathol 2003;119:274-8. (Pubitemid 37047884)
2. Katzmann JA, Abraham RS, Dispenzieri A, Lust JA, Kyle RA. Diagnostic performance of quantitative kappa and lambda free light chain assays in clinical practice. Clin Chem 2005;51:878-81. (Pubitemid 40577925)
3. Katzmann JA, Dispenzieri A, Kyle RA, Snyder MR, Plevak MF, Larson DR, et al. Elimination of the need for urine studies in the screening algorithm for monoclonal gammopathies by using serum immunofixation and free light chain assays. Mayo Clin Proc 2006;81:1575-8. (Pubitemid 44871901)
4. Katzmann JA, Kyle RA, Benson J, Larson DR, Snyder MR, Lust JA, et al. Screening panels for detection of monoclonal gammopathies. Clin Chem 2009;55:1517-22.
5. Dispenzieri A, Lacy MQ, Katzmann JA, Rajkumar SV, Abraham RS, Hayman SR, et al. Absolute values of immunoglobulin free light chains are prognostic in patients with primary systemic amyloidosis undergoing peripheral blood stem cell transplantation. Blood 2006;107:3378-83.
6. Dispenzieri A, Kyle RA, Katzmann JA, Therneau TM, Larson D, Benson J, et al. Immunoglobulin free light chain ratio is an independent risk factor for progression of smoldering (asymptomatic) multiple myeloma. Blood 2008;111:785-9.
7. Rajkumar SV, Kyle RA, Therneau TM, Melton LJ III, Bradwell AR, Clark RJ, et al. Serum free light chain ratio is an independent risk factor for progression in monoclonal gammopathy of undetermined significance. Blood 2005;106:812-7. (Pubitemid 41076419)
8. Abraham RS, Clark RJ, Bryant SC, Lymp JF, Larson T, Kyle RA, et al. Correlation of serum immunoglobulin free light chain quantification with urinary Bence Jones protein in light chain myeloma. Clin Chem 2002;48:655-7. (Pubitemid 34261233)
9. Alyanakian MA, Abbas A, Delarue R, Arnulf B, Aucouturier P. Free immunoglobulin light-chain serum levels in the follow-up of patients with monoclonal gammopathies: correlation with 24-hr urinary light-chain excretion. Am J Hematol 2004;75:246-8. (Pubitemid 38451104)
10. Dispenzieri A, Kyle R, Merlini G, Miguel JS, Ludwig H, Hajek R, et al. International Myeloma Working Group guidelines for serum-free light chain analysis in multiple myeloma and related disorders. Leukemia 2009;23:215-24.
11. Drayson M, Tang LX, Drew R, Mead GP, Carr-Smith H, Bradwell AR. Serum free light-chain measurements for identifying and monitoring patients with nonsecretory multiple myeloma. Blood 2001;97:2900-2.
12. Lachmann HJ, Gallimore R, Gillmore JD, Carr-Smith HD, Bradwell AR, Pepys MB, et al. Outcome in systemic AL amyloidosis in relation to changes in concentration of circulating free immunoglobulin light chains following chemotherapy. Br J Haematol 2003;122:78-84. (Pubitemid 36819341)
13. Mead GP, Carr-Smith HD, Drayson MT, Morgan GJ, Child JA, Bradwell AR. Serum free light chains for monitoring multiple myeloma. Br J Haematol 2004;126:348-54. (Pubitemid 39005714)
14. Jagannath S. Value of serum free light chain testing for the diagnosis and monitoring of monoclonal gammopathies in hematology. Clin Lymphoma Myeloma 2007;7:518-23. (Pubitemid 350043489)
15. Pratt G. The evolving use of serum free light chain assays in haematology. Br J Haematol 2008;141:413-22. (Pubitemid 351550561)
16. Beetham R, Wassell J, Wallage MJ, Whiteway AJ, James JA. Can serum free light chains replace urine electrophoresis in the detection of monoclonal gammopathies? Ann Clin Biochem 2007;44:516-22. (Pubitemid 351724634)
17. Hill PG, Forsyth JM, Rai B, Mayne S. Serum free light chains: an alternative to the urine Bence Jones proteins screening test for monoclonal gammopathies. Clin Chem 2006;52:1743-8. (Pubitemid 44330709)
18. Vermeersch P, Van Hoovels L, Delforge M, Marien G, Bossuyt X. Diagnostic performance of serum free light chain measurement in patients suspected of a monoclonal B-cell disorder. Br J Haematol 2008;143:496-502.
19. Pratt G, Holding S. More studies are needed to assess the performance of serum free light chain measurement for the diagnosis of B-cell disorders in routine clinical practice - response to Vermeersch et al. Br J Haematol 2008;143:145-6.
20. Sheldon J. Free light chains. Ann Clin Biochem 2007;44:503-5. (Pubitemid 351724631)
21. Vermeersch P, Marien G, Bossuyt X. More studies are needed to assess the performance of serum free light chain measurement for the diagnosis of B-cell disorders in routine clinical practice. Br J Haematol 2008;143:143-5.
22. Katzmann JA, Clark RJ, Abraham RS, Bryant S, Lymp JF, Bradwell AR, et al. Serum reference intervals and diagnostic ranges for free kappa and free lambda immunoglobulin light chains: relative sensitivity for detection of monoclonal light chains. Clin Chem 2002;48:1437-44. (Pubitemid 34925779)
23. Hutchison CA, Harding S, Hewins P, Mead GP, Townsend J, Bradwell AR, et al. Quantitative assessment of serum and urinary polyclonal free light chains in patients with chronic kidney disease. Clin J Am Soc Nephrol 2008;3:1684-90.
24. Hofmann W, Garbrecht M, Bradwell AR, Guder WG. A new concept for detection of Bence Jones proteinuria in patients with monoclonal gammopathy. Clin Lab 2004;50:181-5. (Pubitemid 38477543)
25. Fulton RB, Fernando SL. Serum free light chain assay reduces the need for serum and urine immunofixation electrophoresis in the evaluation of monoclonal gammopathy. Ann Clin Biochem 2009;46:407-12.
26. Tate JR, Mollee P, Dimeski G, Carter AC, Gill D. Analytical performance of serum free light-chain assay during monitoring of patients with monoclonal light-chain diseases. Clin Chim Acta 2007;376:30-6. (Pubitemid 44880976)
27. Robson E, Mead G, Carr-Smith H, Bradwell A. In reply to Tate et al. Clin Chim Acta 2007;376:30-6.
28. Robson E, Mead G, Carr-Smith H, Bradwell A. Clin Chim Acta 2007;380:247-1.
29. Abadie JM, Bankson DD. Assessment of serum free light chain assays for plasma cell disorder screening in a Veterans Affairs population. Ann Clin Lab Sci 2006;36:157-62.
30. Shaheen SP, Levinson SS. Serum free light chain analysis may miss monoclonal light chains that urine immunofixation electrophoreses would detect. Clin Chim Acta 2009;406:162-6.
31. Kyle RA, Gertz MA, Witzig TE, Lust JA, Lacy MQ, Dispenzieri A, et al. Review of 1027 patients with newly diagnosed multiple myeloma. Mayo Clin Proc 2003;78:21-33. (Pubitemid 36033957)
32. Hutchison CA, Cockwell P, Reid S, Chandler K, Mead GP, Harrison J, et al. Efficient removal of immunoglobulin free light chains by hemodialysis for multiple myeloma: in vitro and in vivo studies. J Am Soc Nephrol 2007;18:886-95. (Pubitemid 46434505)
33. Bakshi NA, Gulbranson R, Garstka D, Bradwell AR, Keren DF. Serum free light chain (FLC) measurement can aid capillary zone electrophoresis in detecting subtle FLC-producing M proteins. Am J Clin Pathol 2005;124:214-8. (Pubitemid 41060131)