Can serum free light chains replace urine electrophoresis in the detection of monoclonal gammopathies?

Annals of Clinical Biochemistry

Volumn 44, Issue 6, 2007, Pages 516-522

  Beetham, Robert ^a   Wassell, Julie ^a   Wallage, Michael J ^a   Whiteway, Alastair J ^a   James, Jacky A ^a  

^a FRENCHAY HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BENCE JONES PROTEIN; IMMUNOGLOBULIN LIGHT CHAIN; PARAPROTEIN;

ADULT; AGED; ARTICLE; BLOOD ANALYSIS; CONTROLLED STUDY; ELECTROPHORESIS; HUMAN; MAJOR CLINICAL STUDY; MONOCLONAL IMMUNOGLOBULINEMIA; PRIORITY JOURNAL; REFERENCE VALUE; URINALYSIS; ALGORITHM; BLOOD; COMPARATIVE STUDY; EVALUATION; INSTRUMENTATION; METHODOLOGY; MIDDLE AGED; PARAPROTEINEMIA; PROTEIN ELECTROPHORESIS; STANDARD; URINE;

ADULT; AGED; AGED, 80 AND OVER; ALGORITHMS; BENCE JONES PROTEIN; BLOOD PROTEIN ELECTROPHORESIS; ELECTROPHORESIS; HUMANS; IMMUNOGLOBULIN LIGHT CHAINS; MIDDLE AGED; PARAPROTEINEMIAS; REFERENCE VALUES; URINALYSIS;

EID: 40349091724     PISSN: 00045632     EISSN: 00045632     Source Type: Journal    
DOI: 10.1258/000456307782268084     Document Type: Article

References (22)

1. Merlini G. Monoclonal gammopathies. Cancer J 1995; 8: 173-80
2. Solomon A. Bence-Jones proteins and light chains of immunoglobulins. N Engl J Med 1976; 294: 17-23
3. Levinson SS, Keren DF. Free light chains of immunoglobulins: clinical laboratory analysis. Clin Chem 1994; 40: 1869-78
4. Waldmann TA, Strober W, Mogielnicki RP. The renal handling of low molecular weight proteins. J Clin Invest 1972; 51: 2162-74
5. Maack T, Johnson V, Kau ST, Figueiredo J, Sigulem D. Renal filtration, transport and metabolism of low molecular weight proteins: A review. Kidney Int 1979; 16: 251-70
6. Kyle RA, Rajkumar SV. Monoclonal gammopathy of undetermined significance. Br J Haematol 2006; 134: 573-89
7. Bradwell AR. Serum Free Light Chain Analysis. 4th edn. Birmingham UK: The Binding Site 2006
8. Merlini G, Bellotti V. Molecular mechanisms of amyloidosis. N Engl J Med 2003; 349: 583-96
9. Merlini G, Aguzzi F, Whicher J. Monoclonal gammopathies. J Int Fed Clin Chem 1997; 9: 171-6
10. Hill PG, Forsyth JM, Rai B, Mayne S. Serum free light chains: an alternative test to urine Bence Jones proteins when screening for monoclonal gammopathies. Clin Chem 2006; 52: 1743-8
11. McLaughlin P, Alexanian R. Myeloma protein kinetics following chemotherapy. Blood 1982; 60: 851-5
12. Abraham RS, Katzmann JA, Clark RJ, Bradwell AR, Kyle RA, Gertz MA. Quantitative analysis of serum free light chains. A new marker for the diagnostic evaluation of primary systemic amyloidosis. Am J Clin Path 2003; 119: 274-8
13. Drayson M, Tang LX, Drew R, Mead GP, Carr-smith H, Bradwell AR. Serum free light chain measurements for identifying and monitoring patients with non-secretory myeloma. Blood 2001; 97: 2900-2
14. Graziani M, Merlini G, Petrini C. IFCC Committee on Plasma Proteins; SIBioC Study Group on Proteins. Guidelines for the analysis of Bence Jones protein. Clin Chem Lab Med 2003; 41: 338-46
15. Bradwell AR, Carr-Smith HD, Mead GP, et al. Highly sensitive, automated immunoassay for immunoglobulin free light chains in serum and urine. Clin Chem 2001; 47: 673-80
16. Bradwell AR, Carr-Smith HD, Mead GP, Harvey TC, Drayson MT. Serum test for assessment of patients with Bence Jones myeloma. Lancet 2003; 361: 489-91
17. Mead GP, Carr-Smith HD, Drayson MT, Bradwell AR. Detection of Bence Jones myeloma and monitoring of myeloma chemotherapy using immunoassays specific for free immunoglobulin light chains. Clin Lab 2003; 49: 25-7
18. Rajkumar SV, Kyle RA, Therneau L, et al. Serum free light chain ratio is an independent risk factor for progression in monoclonal gammopathy of undetermined significance. Blood 2005; 106: 812-7
19. Katzmann JA, Dispenzieri A, Kyle RA, et al. Elimination of the need for urine studies in the screening algorithm for monoclonal gammopathies by using serum immunofixation and free light chain assays. Mayo Clin Proc 2006; 81: 1575-8
20. Katzmann JA, Clark RJ, Abraham RS, et al. Serum reference intervals and diagnostic ranges for free κ and free λ immunoglobulin light chains: relative sensitivity for detection of monoclonal light chains. Clin Chem 2002; 48: 1437-44
21. Linstedt G, Lundberg P-A. Loss of tubular proteinuria pattern during urine concentration with a commercial membrane filter cell (Minicon B15 system). Clin Chim Acta 1974; 56: 125-6
22. Bakshi NA, Gulbranson R, Gartska D, Bradwell AR, Keren DF. Serum free light chain (FLC) measurement can aid capillary zone electrophoresis in detecting subtle FLC-producing M proteins. Am J Clin Pathol 2005; 124: 214-8