An RNA isolation system for plant tissues rich in secondary metabolites

BMC Research Notes

Volumn 4, Issue , 2011, Pages

  Ghawana, Sanjay ^a,b   Paul, Asosii ^a   Kumar, Hitesh ^a,c   Kumar, Arun ^a   Singh, Harsharan ^a,d   Bhardwaj, Pardeep K ^a,e   Rani, Arti ^a,f   Singh, Ravi S ^a   Raizada, Jyoti ^a   Singh, Kashmir ^a,g   Kumar, Sanjay ^a  

^a CSIR INSTITUTE OF HIMALAYAN BIORESOURCE TECHNOLOGY   (India)

^b NATIONAL INSTITUTE OF PLANT GENOME RESEARCH   (India)

^c SCVB Government College   (India)

^d LYALLPUR KHALSA COLLEGE   (India)

^e INSTITUTE OF BIORESOURCES AND SUSTAINABLE DEVELOPMENT   (India)

^f JAWAHARLAL NEHRU CENTRE FOR ADVANCED SCIENTIFIC RESEARCH   (India)

^g PANJAB UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ARNEBIA; ARNEBIA EUCHROMA; RHEUM; RHEUM AUSTRALE; RHEUM X HYBRIDUM;

EID: 79953084237     PISSN: None     EISSN: 17560500     Source Type: Journal    
DOI: 10.1186/1756-0500-4-85     Document Type: Article

References (23)

1. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. P Chomczynski N Sacchi, Anal Biochem 1987 162 156 159 10.1016/0003-2697(87)90021-2 2440339 (Pubitemid 17064313)
2. A simple and efficient method for isolating RNA from pine trees. S Chang J Puryear J Cairney, Plant Mol Biol Rep 1993 11 113 116 10.1007/BF02670468
3. RNA isolation from plant tissues recalcitrant to extraction in guanidine. RC Bugos VL Chiang XH Zhang ER Campbell GK Podila WH Campbell, BioTechniques 1995 19 734 737 8588907
4. An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. RA Salzman T Fujita K Zhu-Salzman PM Hasegawa RA Bressan, Plant Mol Biol Rep 1999 17 11 17 10.1023/A:1007520314478 (Pubitemid 129640666)
5. RNA isolation from high-phenolic tea leaves and apical buds. L Lal R Sahoo RK Gupta P Sharma S Kumar, Plant Mol Biol Rep 2001 19 181a 181f 10.1007/BF02772161 (Pubitemid 33695171)
6. Development of an efficient protocol of RNA isolation from recalcitrant tree tissues. X Wang W Tian Y Li, Mol Biotechnol 2008 38 57 64 10.1007/s12033-007-0073-6 18095190 (Pubitemid 351378096)
7. Using silica particles to isolate total RNA from plant tissues recalcitrant to extraction in guanidine thiocyanate. LW Ding QY Sun ZY Wang YB Sun ZF Xu, Anal Biochem 2008 374 426 428 10.1016/j.ab.2007.11.030 18166144
8. Overcoming problems of phenolic and quinones in the isolation of plant enzymes and organelles. WD Loomis, Methods Enzymol 1974 31 528 545 full-text 4418408
9. A method for rapid isolation of RNA and a kit thereof. S Ghawana K Singh J Raizada A Rani P Bhardwaj S Kumar, International Publication No. WO 2007/113614 2007
10. Molecular Cloning: A Laboratory Manual. J Sambrook EF Fritsch T Maniatis, Cold Spring Harbor, Cold Spring Harbor Press 1989
11. Isolation of good quality RNA from a medicinal plant seabuckthorn rich in secondary metabolites. R Ghansal S Raghuvanshi PC Sharma, Plant Physiol Biochem 2009 47 113 115
12. A method for isolation of total RNA from fruit tissues of banana. J-J Liu C-J Goh C-S Loh P Liu E-C Pua, Plant Mol Biol Rep 1998 16 1 6 10.1023/A:1017158311412 (Pubitemid 128110177)
13. Characterization of gene expression of QM from Caragana jubata, a plant species that grows under extreme cold. PK Bhardwaj PS Ahuja S Kumar, Mol Biol Rep 2010 37 1003 1010 10.1007/s11033-009-9791-0 19757181
14. -26 S rRNA-based internal control gene primer pair for reverse transcription-polymerase chain reaction-based quantitative expression studies in diverse plant species. K Singh J Raizada P Bhardwaj S Ghawana A Rani H Singh K Kaul S Kumar, Anal Biochem 2004 335 330 333 10.1016/j.ab.2004.08.030 15556573 (Pubitemid 39535199)
15. Early low-temperature responsive mitogen activated protein kinases RaMPK1 and RaMPK2 from Rheum australe D. Don respond differentially to diverse stresses. S Ghawana S Kumar PS Ahuja, Mol Biol Rep 2010 37 933 938 10.1007/s11033-009-9726-9 19688272
16. Light and temperature regulated terpene biosynthesis: hepatoprotective monoterpene picroside accumulation in Picrorhiza kurrooa. T Kawoosa H Singh A Kumar SK Sharma K Devi S Dutt SK Vats M Sharma S Kumar PS Ahuja, Funct Integr Genomics 2010 10 393 404 10.1007/s10142-009-0152-9 20076984
17. Expression of 3-hydroxy-3-methylglutaryl-CoA reductase, p-hydroxybenzoate-m-geranyltransferase and genes of phenylpropanoid pathway exhibits positive correlation with shikonins content in arnebia [Arnebia euchroma (Royle) Johnston]. RS Singh RK Gara PK Bhardwaj A Kaachra S Malik R Kumar M Sharma PS Ahuja S Kumar, BMC Mol Biol 2010 11 88 10.1186/1471-2199-11-88 21092138
18. Data for Biochemical Research. RMC Dawson DC Elliott WH Elliott KM Jones, Oxford Science Publication 1986
19. Large and small-scale phenol extractions. DM Wallace, Methods Enzymol 1987 152 33 41 full-text 2443801
20. A reliable and efficient Method for total RNA isolation from various members of Spurge family (Euphorbiaceae). J Xu M Aileni S Abbagani P Zhang, Phytochem Anal 2010 21 395 398 10.1002/pca.1205 20135710
21. A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites. E Kiefer W Heller D Ernst, Plant Mol Biol Rep 2000 18 33 39 10.1007/BF02825291
22. p-Coumarate:CoA ligase as a key gene in the yield of catechins in tea [Camellia sinensis (L.) O. Kuntze]. A Rani K Singh P Sood S Kumar PS Ahuja, Funct Integr Genomics 2009 9 271 275 10.1007/s10142-008-0098-3 18931865
23. Phenylalanine ammonia-lyase (PAL) and cinnamate 4-hydroxylase (C4H) and catechins (flavan-3-ols) accumulation in tea. K Singh S Kumar A Rani A Gulati PS Ahuja, Funct Integr Genomics 2009 9 125 134 10.1007/s10142-008-0092-9 18679731