An Improved RNA Isolation Method for Plant Tissues Containing High Levels of Phenolic Compounds or Carbohydrates

Plant Molecular Biology Reporter

Volumn 17, Issue 1, 1999, Pages 11-17

  Salzman, R A ^a   Fujita, T ^a   Zhu Salzman, K ^a   Hasegawa, P M ^a   Bressan, R A ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

Albizia; Griffonia; Phenolics; Recalcitrant tissues; RNA extraction; Vitis

Indexed keywords

ALBIZIA; ALBIZIA PROCERA; GRIFFONIA; GRIFFONIA SIMPLICIFOLIA; RAPHANUS SATIVUS; VITIS; VITIS SP.;

EID: 0033409606     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007520314478     Document Type: Review

References (8)

1. Chang S, Puryear J and Cairney J (1993) A simple and efficient method for isolating RNA from pinetrees. Plant Mol Biol Reptr 11: 113-116.
2. Chomczynski P and Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
3. Lewinsohn E, Steele C and Croteau R (1994) Simple isolation of functional RNA from woody stems of gymnosperms. Plant Mol Biol Reptr 12: 20-25.
4. Loomis WD (1974) Overcoming problems of phenolics and quinones in the isolation of plant enzymes and organelles. Meth Enzymol 31: 528-545.
5. Terras FRG, Eggermont K, Kovaleva V, Raikhel NV, Osborn RW, Kester A, Rees SB, Torrekens S, VanLeuven F, Vanderleyden J, Cammue BPA and Broekaert WF (1995) Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell 7: 573-588.
6. Tesniere C and Vayda ME (1991) Method for the isolation of high-quality RNA from grape berry tissues without contaminating tannins or carbohydrates. Plant Mol Biol Reptr 9: 242-251.
7. VanDriessche ES, Beeckmans R, Dejaegere R and Kanarek L (1984) Thiourea: The antioxidant of choice for the purification of proteins from phenol-rich tissues. Anal Biochem 141: 184-188.
8. Woodhead M, Taylor MA, Davies HV, Brennan RM and McNicol RJ (1997) Isolation of RNA from blackcurrant (Ribes nigrum L.) fruit. Mol Biotechnol 7(1): 1-4.