Isolation of good quality RNA from a medicinal plant seabuckthorn, rich in secondary metabolites

Plant Physiology and Biochemistry

Volumn 47, Issue 11-12, 2009, Pages 1113-1115

  Ghangal, Rajesh ^a   Raghuvanshi, Saurabh ^b   Chand Sharma, Prakash ^a  

^a GURU GOBIND SINGH INDRAPRASTHA UNIVERSITY   (India)

^b UNIVERSITY OF DELHI   (India)

Author keywords

CTAB; Medicinal plants; RNA isolation; Seabuckthorn; Secondary metabolites

Indexed keywords

ACETIC ACID; BUFFER; CETRIMIDE; RNA;

ARTICLE; BIOTECHNOLOGY; GENETICS; HIPPOPHAE RHAMNOIDES; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; VALIDATION STUDY;

ACETIC ACID; BIOTECHNOLOGY; BUFFERS; CETRIMONIUM COMPOUNDS; HIPPOPHAE; RNA;

HIPPOPHAE; HIPPOPHAE RHAMNOIDES;

EID: 71849084733     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2009.09.004     Document Type: Article

References (16)

1. Zeb A. Important therapeutic uses of sea buckthorn (Hippophae): a review. J. Biol. Sci. 4 (2004) 687-693
2. Lu R. Seabuckthorn a multipurpose plant for fragile mountains, ICIMOD occasional paper no. 20 Kathmandu, Nepal; 1992.
3. Zhang J.T., and Chen T. Effects of mixed Hippophae rhamnoides on community and soil in planted forests in the Eastern Loess Plateau-China. Ecol. Eng. 31 (2007) 115-121
4. Yoshida T., Tanaka K., Chen X.M., and Okuda T. Tannins from Hippophae rhamnoides. Phytochemistry 30 (1991) 663-666
5. Tiitinen K.M., Hakala M.A., and Kallio H.P. Quality components of sea buckthorn (Hippophae rhamnoides) varieties. J. Agric. Food Chem. 53 (2005) 1692-1699
6. Loomis W.D. Overcoming problems of phenolics in the isolation of plant enzymes and organelles. Methods Enzymol. 31 (1974) 528-545
7. Chomczynski P., and Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162 (1987) 156-159
8. Liu J.J., Goh C.J., Loh C.S., Liu P., and Pua E.C. A method for isolation of total RNA from fruit tissues of banana. Plant Mol. Biol. Rep. 16 (1998) 1-6
9. Chang S., Puryear J., and Cairney J. A simple and efficient method for isolating RNA from pine trees. Plant Mol. Biol. Rep. 11 (1993) 113-116
10. Salzman R.A., Fujita T., Salzman K.Z., Hasegawa P.M., and Bressan R.A. An improved RNA isolation method for plant tissues containing high levels of phenolic compounds or carbohydrates. Plant Mol. Biol. Rep. 17 (1999) 11-17
11. Maliyakal E.J. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Res. 20 (1992) 2381
12. Venkatachalam P., Thanseem I., and Thulaseedharan A. A rapid and efficient method for isolation of RNA from bark tissues of Hevea brasiliensis. Curr. Sci. 77 (1999) 635-637
13. Lal L., Sahoo R., Gupta R.K., Sharma P., and Kumar S. RNA isolation from high-phenolic tea leaves and apical buds. Plant Mol. Biol. Rep. 19 (2001) 181a-f
14. Birnboim H.C. Extraction of high molecular weight RNA and DNA from cultured mammalian cells. Methods Enzymol. 216 (1992) 154-160
15. Chen G.Y.J., Jin S., and Goodwin P.H. An improved method for the isolation of total RNA from Malva pusilla tissues infected with Colletotrichum gloeosporioides. J. Phytopathol. 148 (2000) 57-60
16. Aljanabi S.M., Forget L., and Dookun A. An improved rapid protocol for the isolation of polysaccharide and polyphenol-free sugarcane DNA. Plant Mol. Biol. Rep. 17 (1999) 1-8