Pathogen quantitation in complex matrices: A multi-operator comparison of DNA extraction methods with a novel assessment of PCR inhibition

PLoS ONE

Volumn 6, Issue 3, 2011, Pages

  Pontiroli, Alessandra ^a   Travis, Emma Rachel ^a   Sweeney, Francis Patrick ^a   Porter, David ^a   Gaze, William Hugo ^a   Mason, Sam ^a   Hibberd, Victoria ^a   Holden, Jennifer ^a   Courtenay, Orin ^a   Wellington, Elizabeth Margaret Helen ^a  

^a UNIVERSITY OF WARWICK   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CHLOROFORM; PHENOL; BACTERIAL DNA;

ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BOVINE TUBERCULOSIS; CONTROLLED STUDY; DNA EXTRACTION; DNA PURIFICATION; FECES ANALYSIS; INTERMETHOD COMPARISON; MYCOBACTERIUM BOVIS; NONHUMAN; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; SENSITIVITY AND SPECIFICITY; SOIL ANALYSIS; ANALYTICAL EQUIPMENT; ANIMAL; ANIMAL DISEASE; ARTIFACT; CATTLE; COMPARATIVE STUDY; COST BENEFIT ANALYSIS; ECONOMICS; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; STANDARD;

BOS; BOVINAE; MYCOBACTERIUM BOVIS;

ANIMALS; ARTIFACTS; CATTLE; COST-BENEFIT ANALYSIS; DNA, BACTERIAL; MYCOBACTERIUM BOVIS; POLYMERASE CHAIN REACTION; REAGENT KITS, DIAGNOSTIC; SENSITIVITY AND SPECIFICITY;

EID: 79952907122     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017916     Document Type: Article

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