Plasmid DNA vaccination using skin electroporation promotes poly-functional CD4 T-cell responses

Immunology and Cell Biology

Volumn 89, Issue 3, 2011, Pages 492-496

  Brve, Andreas ^a   Nyström, Sanna ^b   Roos, Anna Karin ^c   Applequist, Steven E ^b  

^a SWEDISH INSTITUTE FOR INFECTIOUS DISEASE CONTROL   (Sweden)

^b KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^c KAROLINSKA INSTITUTE   (Sweden)

Author keywords

DNA vaccination; electroporation; poly functional; skin; T cells

Indexed keywords

GAG PROTEIN; PLASMID DNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CD4+ T LYMPHOCYTE; CELLULAR IMMUNITY; CONTROLLED STUDY; DNA IMMUNIZATION; ELECTROPORATION; ENZYME LINKED IMMUNOSPOT ASSAY; FEMALE; FLOW CYTOMETRY; GENE EXPRESSION; HUMORAL IMMUNITY; IMMUNE RESPONSE; MOUSE; NONHUMAN; VACCINATION;

ANIMALS; CD4-POSITIVE T-LYMPHOCYTES; CYTOKINES; ELECTROPORATION; FEMALE; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; GENETIC VECTORS; HIV-1; HUMANS; IMMUNITY, CELLULAR; IMMUNITY, HUMORAL; INJECTIONS, INTRAMUSCULAR; MICE; MICE, INBRED BALB C; MICE, INBRED C57BL; PLASMIDS; SKIN; SPLEEN; VACCINATION; VACCINES, DNA;

HUMAN IMMUNODEFICIENCY VIRUS;

EID: 79952813888     PISSN: 08189641     EISSN: 14401711     Source Type: Journal    
DOI: 10.1038/icb.2010.109     Document Type: Article

References (8)

1. Kutzler MA, Weiner DB. DNA vaccines: Readyfor primetime? Nat Rev Genet 2008; 9: 776-788.
2. Babiuk S, Baca-Estrada ME, Foldvari M, Baizer L, Stout R, Storms M et al. Needle-Free topical electroporation improves gene expression from plasmids administered in porcineskin. Mol Ther 2003; 8: 992-998. (Pubitemid 38072312)
3. Roos AK, Moreno S, Leder C, Pavlenko M, King A, Pisa P. Enhancement of cellular immuneresponseto a prostatecancer DNA vaccineby intradermal electroporation. Mol Ther 2006; 13: 320-327. (Pubitemid 43056897)
4. Hooper JW, Golden JW, Ferro AM, King AD. Smallpox DNA vaccine delivered by novel skin electroporation device protects miceagainst intranasal poxviruschallenge. Vaccine 2007; 25:1814-1823. (Pubitemid 46176327)
5. Hirao LA, Wu L, Khan AS, Satishchandran A, Draghia-Akli R, Weiner DB. Intradermal/subcutaneous immunization by electroporation improves plasmid vaccine delivery and potency in pigs and rhesus macaques. Vaccine 2008; 26: 440-448.
6. Drabick JJ, Glasspool-Malone J, King A, Malone RW. Cutaneous trans-fection and immune responses to intradermal nucleic acid vaccination are significantly enhanced by in vivo electropermeabilization. Mol Ther 2001; 3: 249-255. (Pubitemid 32509828)
7. Seder RA, Darrah PA, Roederer M. T-Cell quality in memory and protection: Implica-tions for vaccine design. Nat Rev Immunol 2008; 8: 247-258. (Pubitemid 351432801)
8. Roos AK, Eriksson F, Walters DC, Pisa P, King AD. Optimization of skin electroporation in miceto increasetolerability of DNA vaccinedelivery to patients. Mol Ther 2009; 17: 1637-1642.