Equilibrium adsorption of poly(His)-tagged proteins on immobilized metal affinity chromatographic adsorbents

Biochemical Engineering Journal

Volumn 54, Issue 1, 2011, Pages 1-9

  Yang, Yi Hsing ^a   Wu, Tusng Ta ^a   Suen, Shing Yi ^a   Lin, Sung Chyr ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Adsorption isotherm; Hydroxyapatite; Immobilized metal affinity chromatography; Langmuir; Langmuir Freundlich

Indexed keywords

ADSORPTION BEHAVIOR; ADSORPTION CAPACITIES; CONTACT SURFACE AREA; DENATURING CONDITIONS; DISSOCIATION CONSTANT; EQUILIBRIUM ADSORPTION; EXPERIMENTAL DATA; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; IMMOBILIZED METALS; LANGMUIR ISOTHERM MODELS; LANGMUIR-FREUNDLICH ISOTHERM MODEL; LANGMUIRS; LARGE-SCALE APPLICATIONS; MODEL PROTEINS; MULTIPOINT ATTACHMENT; PHOSPHATE BUFFERS; QUATERNARY STRUCTURE; RACEMASE; RECOMBINANT PROTEIN; RECOVERY YIELD; SYSTEMATIC ANALYSIS; TAGGED PROTEINS; THREE MODELS;

ADSORBENTS; ADSORPTION; ADSORPTION ISOTHERMS; AFFINITY CHROMATOGRAPHY; APATITE; DISSOCIATION; HYDROXYAPATITE; METALS; OLIGOMERS;

PROTEINS;

ADSORBENT; HYDROXYAPATITE; IMIDAZOLE; POLYHISTIDINE TAG; RECOMBINANT PROTEIN;

ADSORPTION KINETICS; ARTICLE; BINDING AFFINITY; CHEMICAL MODEL; DISSOCIATION CONSTANT; ELUTION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; PH; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE;

EID: 79952735254     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2010.12.005     Document Type: Article

References (37)

1. Porath J., Carlsson J., Olsson I., Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 1975, 258:598-599.
2. Hochuli E., Dobeli H., Schacher A. New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues. J. Chromatogr. 1987, 411:177-184.
3. Chaga G.S. Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. J. Biochem. Biophys. Methods 2001, 49:313-334.
4. Gaberc-Porekar V., Menart V. Perspectives of immobilized-metal affinity chromatography. J. Biochem. Biophys. Methods 2001, 49:335-360.
5. Brena B.M., Ryden L.G., Porath J. Immobilization of beta-galactosidase on metal-chelate-substituted gels. Biotechnol. Appl. Biochem. 1994, 17:217-231.
6. Ordaz E., Garrido-Pertierra A., Gallego M., Puyet A. Covalent and metal-chelate immobilization of a modified 2-haloacid dehalogenase for the enzymatic resolution of optically active chloropropionic acid. Biotechnol. Prog. 2000, 16:287-291.
7. Ho L.F., Li S.Y., Lin S.C., Hsu W.H. Integrated enzyme purification and immobilization processes with immobilized metal affinity adsorbents. Proc. Biochem. 2004, 39:1573-1581.
8. Lemercier G., Bakalara N., Santarelli X. On-column refolding of an insoluble histidine tag recombinant exopolyphosphatase from Trypanosoma brucei overexpressed in Escherichia coli. J. Chromatogr. B 2003, 786:305-309.
9. Jungbauer A., Kaar W., Schlegl R. Folding and refolding of proteins in chromatographic beds. Curr. Opin. Biotechnol. 2004, 15:487-494.
10. Hefti M.H., Milder F.J., Boeren S., Vervoot J., van Berkel W.J.H. A His-tag based immobilization method for the preparation and reconstitution of apoflavoproteins. Biochim. Biophys. Acta 2003, 1619:139-143.
11. Bernard G. Methods in Enzymology 1971, vol. 22:325-342. Academic Press, New York.
12. Gorbunoff M.J., Timasheff S.N. The interaction of proteins with hydroxyapatite. III. Mechanism. Anal. Biochem. 1984, 136:440-445.
13. Ivanov I., Gigova L. Isolation of l phage DNA by hydroxyapatite chromatography. Anal. Biochem. 1985, 146:389-392.
14. Nordström T., Senkas A., Eriksson S., Pontyne N., Nordström E., Lindqvist C. Generation of a new protein purification matrix by loading ceramic hydroxyapatite with metal ions-demonstration with poly-histidine tagged green fluorescent protein. J. Biotechnol. 1999, 69:125-133.
15. Suen R.B., Lin S.C., Hsu W.H. Hydroxyapatite-based immobilized metal affinity adsorbents for protein purification. J. Chromatogr. A 2004, 1048:31-39.
16. Lin P.C., Lin S.C., Hsu W.H. Adsorption behaviors of recombinant proteins on hydroxyapatite-based immobilized metal affinity chromatographic adsorbents. J. Chin. Inst. Chem. Eng. 2008, 39:389-398.
17. S.F. Chen, Expression of Recombinant N-acyl-d-Glucosamine 2-Epimerase in Escherichia coli, M.S. Thesis, National Chung Hsing University, Taichung, Taiwan, 1999.
18. Hu H.Y., Hsu W.H., Chien H.C.R. Characterization and phylogenetic analysis of a thermostable N-carbamoyl-l-amino acid amidohydrolase from Bacillus kaustophilus CCRC11223. Arch. Microbiol. 2003, 179:250-257.
19. Wang W.C., Chiu W.C., Hsu S.K., Wu C.L., Chen C.Y., Lin J.S., Hsu W.H. Structural basis for catalytic racemization and substrate specificity of an N-acylamino acid racemase homologue from Deinococcus radiodurans. J. Mol. Biol. 2004, 342:155-169.
20. Ivanov A.E., Galaev I.Y., Kazakov S.V., Mattissaon B. Thermosensitive copolymer of N-vinylcaprolactam and 1-vinylimidazole: molecular characterization and separation by immobilized metal affinity chromatography. J. Chromatogr. A 2001, 907:115-130.
21. Mateo C., Fernandez-Lorente G., Pessela B.C., Vian A., Carrascosa A.V., Garcia J.L., Fernandez-Lafuente R., Guisan J.M. Affinity chromatography of polyhistidine tagged enzymes-New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions. J. Chromatogr. A 2001, 915:97-106.
22. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.
23. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
24. Hjerten S., Levin O., Tiselius A. Protein chromatography on calcium phosphate columns. Arch. Biochem. Biophys. 1956, 65:132-155.
25. Horstmann B.J., Kenney C.N., Chase H.A. Adsorption of proteins on Sepharose affinity adsorbents of varying particle size. J. Chromatogr. 1986, 361:179-190.
26. Anspach F.B., Johnston A., Wirth H.J., Unger K.K., Hearn M.T.W. High-performance liquid chromatography of amino acids, peptides and proteins. XCII. Thermodynamic and kinetic investigations on rigid and soft affinity gels with varying particle and pore sizes. J. Chromatogr. 1989, 476:205-225.
27. Yoshida H., Kataoka T. Adsorption of BSA on cross-linked chitosan: the equilibrium isotherm. Chem. Eng. J. 1989, 41:11-15.
28. Johnson R.D., Arnold F.H. The Temkin isotherm describes heterogeneous protein adsorption. Biochim. Biophys. Acta 1995, 1247:293-297.
29. Cantor C.R., Schimmel P.R. Biophysical Chemistry. Part III. The Behavior of Biological Macromolecules 1980, Freeman, New York.
30. Sharma S., Agarwal G.P. Interactions of proteins with immobilized metal ions: a comparative analysis using various isotherm models. Anal. Biochem. 2001, 288:126-140.
31. Goel A., Colcher D., Koo J.S., Booth B.J.M., Pavlinkova G., Batra S.K. Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct. Biochim. Biophys. Acta 2000, 1523:13-20.
32. Itoh T., Mikami B., Maru I., Ohta Y., Hashimoto W., Murata K. Crystal structure of N-acyl-d-glucosamine 2-epimerase from porcine kidney at 2.0Å resolution. J. Mol. Biol. 2000, 303:733-744.
33. Friguet B., Chaffotte A.F., Djavadi-Ohaniance L., Goldberg M.E. Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J. Immunol. Methods 1985, 77:305-319.
34. Todd R.J., Johnson R.D., Arnold F.H. Multiple-site binding interactions in metal-affinity chromatography. I. Equilibrium binding of engineered histidine-containing cytochromes c. J. Chromatogr. A 1994, 662:13-26.
35. Chen C.Y., Chiu W.C., Lin J.S., Hsu W.H., Wang W.C. Structural basis for catalysis and substrate specificity of Agrobacterium radiobacter N-carbamoyl-d-amino acid amidohydrolase. J. Biol. Chem. 2003, 278:26194-26201.
36. Finette G.M.S., Mao Q.M., Hearn M.T.W. Comparative studies on the isothermal characteristics of proteins adsorbed under batch equilibrium conditions to ion-exchange, immobilised metal ion affinity and dye affinity matrices with different ionic strength and temperature conditions. J. Chromatogr. A 1997, 763:71-90.
37. Urist M.R., Huo Y.K., Brownell A.G., Hohl W.M., Buyske J., Lietze A., Tempst P., Hunkapiller M., DeLange R.J. Purification of bovine bone morphogenetic protein by hydroxyapatite chromatography. PNAS 1984, 81:371-375.