Generation of a new protein purification matrix by loading ceramic hydroxyapatite with metal ions - Demonstration with poly-histidine tagged green fluorescent protein

Journal of Biotechnology

Volumn 69, Issue 2-3, 1999, Pages 125-133

  Nordström, Tommy ^a   Senkas, Annika ^a   Eriksson, Susann ^a   Pöntynen, Nora ^a   Nordström, Egon ^a   Lindqvist, Christer ^a  

^a ÅBO AKADEMI UNIVERSITY   (Finland)

Author keywords

Baculovirus; GFP; Hydroxyapatite; Metal ions; Poly histidine

Indexed keywords

CERAMIC MATERIALS; GENETIC ENGINEERING; IRON; NICKEL; PHOSPHATE MINERALS; PURIFICATION; VIRUSES; ZINC;

BACULOVIRUS; GREEN FLUORESCENT PROTEINS; HYDROXYAPATITE;

PROTEINS;

GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; HYDROXYAPATITE; METAL ION; POLYHISTIDINE; VIRUS VECTOR;

ARTICLE; BACULOVIRUS; CELL LINE; GENETIC TRANSFECTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; INSECT; MATERIALS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; TECHNIQUE;

ANIMALS; CELL LINE; CERAMICS; CHROMATOGRAPHY, AFFINITY; DURAPATITE; FERRIC COMPOUNDS; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HISTIDINE; IMMUNOBLOTTING; LUMINESCENT PROTEINS; METALS; NICKEL; NUCLEOPOLYHEDROVIRUS; PEPTIDES; PROMOTER REGIONS (GENETICS); PROTEIN BINDING; PROTEINS; RECOMBINANT FUSION PROTEINS; SPODOPTERA; VIRAL PROTEINS; ZINC;

AEQUOREA VICTORIA; AUTOGRAPHA CALIFORNICA; AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; CYPOVIRUS; INSECTA; SPODOPTERA FRUGIPERDA; UNIDENTIFIED BACULOVIRUS; UNIDENTIFIED NUCLEAR POLYHEDROSIS VIRUSES; VICTORIA;

EID: 0033044347     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(99)00038-3     Document Type: Article

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