Toward an on-chip multiplexed nucleic acid hybridization assay using immobilized quantum dot-oligonucleotide conjugates and fluorescence resonance energy transfer

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7909, Issue , 2011, Pages

  Tavares, Anthony J ^a   Noor, M Omair ^a   Algar, W Russ ^a   Vannoy, Charles H ^a   Chen, Lu ^a   Krull, Ulrich J ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Bioconjugate; Electroosmotic flow; Hybridization; Immobilization; Microfluidics; Nucleic acids; Quantum dots

Indexed keywords

BIOCONJUGATES; ELECTROOSMOTIC FLOW; HYBRIDIZATION; IMMOBILIZATION; QUANTUM DOTS;

ASSAYS; BIOMOLECULES; ELECTROOSMOSIS; ENERGY TRANSFER; FLUIDIC DEVICES; FLUORESCENCE; MICROFLUIDICS; MULTIPLEXING; NUCLEIC ACID SEQUENCES; NUCLEIC ACIDS; OLIGONUCLEOTIDES; ORGANIC POLYMERS; PROBES; RESONANCE;

SEMICONDUCTOR QUANTUM DOTS;

EID: 79952686740     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.877326     Document Type: Conference Paper

References (26)

1. Medintz, I.L., Pons, T., Susumu, K., Boeneman, K., Dennis, A.M., Farrell, D., Deschamps, J.R., Melinger, J.S., Bao, G. and Mattoussi, H., "Resonance energy transfer between luminescent quantum dots and diverse fluorescent protein acceptors," J. Phys. Chem. C, 113(43), 18552-18561 (2009).
2. Medintz, I.L., Uyeda, H.T., Goldman, E.R. and Mattoussi, H., "Quantum dot bioconjugates for imaging, labelling and sensing," Nat. Mater., 4(6), 435-446 (2005). (Pubitemid 40774047)
3. Chan, W.C.W. and Nie, S., "Quantum dot bioconjugates for ultrasensitive nonisotopic detection," Science, 281(5385), 2016-2018 (1998). (Pubitemid 28475486)
4. Medintz, I.L., Clapp, A.R., Mattoussi, H., Goldman, E.R., Fisher, B. and Mauro, J.M., "Self-assembled nanoscale biosensors based on quantum dot FRET donors," Nat. Mater., 2(9), 630-638 (2003).
5. Algar, W.R. and Krull, U.J., "Quantum dots as donors in fluorescence resonance energy transfer for the bioanalysis of nucleic acids, proteins, and other biological molecules," Anal. Bioanal. Chem., 391(5), 1609-1618 (2008).
6. Algar, W.R. and Krull, U.J., "New opportunities in multiplexed optical bioanalyses using quantum dots and donor acceptor interactions," Anal. Bioanal. Chem., 398, 2439-2449 (2010).
7. Algar, W.R., Massey, M. and Krull, U.J., "The application of quantum dots, gold nanoparticles and molecular switches to optical nucleic-acid diagnostics," TrAC Trends Anal. Chem., 28(3), 292-306 (2009).
8. Algar, W.R., Tavares, A.J. and Krull, U.J., "Beyond labels: A review of the application of quantum dots as integrated components of assays, bioprobes, and biosensors utilizing optical transduction," Anal. Chim. Acta, 673(1), 1-25 (2010).
9. Algar, W.R. and Krull, U.J., "Towards multi-color strategies for the detection of oligonucleotide hybridization using quantum dots as energy donors in fluorescence resonance energy transfer (FRET)," Anal. Chim. Acta, 581(2), 193-201 (2007). (Pubitemid 44838460)
10. Algar, W.R. and Krull, U.J., "Toward a multiplxed solid-phase nucleic acid hybridization assay using quantum dots as donors in fluorescence resonance energy transfer," Anal. Chem., 81(10), 4113-4120 (2009).
11. Algar, W.R. and Krull, U.J., "FRET-based solid-phase three-color and four-color hybridization assays using mixed films of quantum dots and oligonucleotides," Mater. Res. Soc. Sym. Proc. 1241, 45-55 (2010).
12. Algar, W.R. and Krull, U.J., "Interfacial transduction of nucleic acid hybridization using immobilized quantum dots as donors in fluorescence resonance energy transfer," Langmuir, 25(1), 633-638 (2009).
13. Algar, W.R. and Krull, U.J., "Developing mixed films of immobilized oligonucleotides and quantum dots for the multiplexed detection of nucleic acid hybridization using a combination of fluorescence resonance energy transfer and direct excitation of fluorescence," Langmuir, 26(8), 6041-6047 (2010).
14. Algar, W.R. and Krull, U.J., "Multiplexed interfacial transduction of nucleic acid hybridization using a single color of immobilized quantum dot donor and two acceptors in fluorescence resonance energy transfer," Anal. Chem., 82(1), 400-405 (2010).
15. Erickson, D., Liu, X., Krull, U.J. and Li, D., "Electrokinetically controlled DNA hybridization microfluidic chip enabling rapid target analysis," Anal. Chem., 76(24), 7269-7277 (2004). (Pubitemid 39657997)
16. Tavares, A.J., Petryayeva, E., Algar, W.R., Chen, L. and Krull, U.J., "Toward a hybridization assay using fluorescence resonance energy transfer and quantum dots immobilized in microfluidic channels," Proc. SPIE. 7750, 775003 (2010).
17. Chen, L., Algar, W.R., Tavares, A.J. and Krull, U.J., "Toward a solid-phase nucleic acid hybridization assay within microfluidic channels using immobilized quantum dots as donors in fluorescence resonance energy transfer," Anal. Bioanal. Chem., In press., (2010).
18. Watterson, J.H., Raha, S., Kotoris, C.C., Wust, C.C., Gharabaghi, F., Jantzi, S.C., Haynes, N.K., Gendron, N.H., Krull, U.J., Mackenzie, A.E. and Piunno, P.A.E., "Rapid detection of single nucleotide polymorphisms associated with spinal muscular atrophy by use of a reusable fibre-optic biosensor," Nucleic Acids Res., 32(2), e18 (2004).
19. Huang, G.-W., Chen, C.-Y., Wu, K.-C., Ahmed, M.O. and Chou, P.-T., "One-pot synthesis and characterization of high-quality CdSe/ZnX (X = S, Se) nanocrystals via the CdO precursor," J. Cryst. Growth, 265(1-2), 250-259 (2004).
20. Peng, Z.A. and Peng, X., "Formation of high-quality CdTe, CdSe, and CdS nanocrystals using CdO as precursor," J. Am. Chem. Soc., 123(1), 183-184 (2001). (Pubitemid 32052980)
21. Pong, B.-K., Trout, B.L. and Lee, J.-Y., "Modified ligand-exchange for efficient solubilization of CdSe/ZnS quantum dots in water: A procedure guided by computational studies," Langmuir, 24(10), 5270-5276 (2008).
22. Yu, W.W., Qu, L., Guo, W. and Peng, X., "Experimental determination of the extinction coefficient of CdTe, CdSe, and CdS nanocrystals," Chem. Mater., 15(14), 2854-2860 (2003).
23. Algar, W.R. and Krull, U.J., "Multidentate surface ligand exchange for the immobilization of CdSe/ZnS quantum dots and surface quantum dot-oligonucleotide conjugates," Langmuir, 24(10), 5514-5520 (2008).
24. Erickson, D., Liu, X., Venditti, R., Li, D. and Krull, U.J., "Electrokinetically based approach for singlenucleotide polymorphism discrimination using a microfluidic device," Anal. Chem., 77(13), 4000-4007 (2005). (Pubitemid 40934264)
25. Algar, W.R. and Krull, U.J., "Adsorption and hybridization of oligonucleotides on mercaptoacetic acid-capped CdSe/ZnS quantum dots and quantum dot-oligonucloetide conjugates," Langmuir, 22(26), 11346-11352 (2006). (Pubitemid 46129679)
26. Zhu, Y.C., Cheng, G.J. and Dong, S.J., "Electric field-induced conformational transition of bovine serum albumin from α-helix to β-sheet," Chin. Chem. Lett., 11(2), 149-152 (2000)