Isolation, screening, and selection of an L-glutaminase producer from soil and media optimization using a statistical approach

Biotechnology and Bioprocess Engineering

Volumn 15, Issue 6, 2010, Pages 975-983

  Padma, Iyer ^a   Singhal, Rekha ^a  

^a INSTITUTE OF CHEMICAL TECHNOLOGY   (India)

Author keywords

glutaminase; Klebsiella oxytoca; response surface methodology; screening

Indexed keywords

16S RRNA; AMMONIUM ACETATE; BEEF EXTRACTS; CENTRAL COMPOSITE DESIGNS; GARDEN SOILS; GLUTAMINASE; INTERACTIVE EFFECT; KLEBSIELLA OXYTOCA; MEDIA OPTIMIZATION; ONE-FACTOR; OPTIMIZED MEDIA; RESPONSE SURFACE METHODOLOGY; SPECIFIC ACTIVITY; STATISTICAL APPROACH; YEAST EXTRACTS;

ENZYME ACTIVITY; MALTOSE; OPTIMIZATION; RNA; SODIUM; SOILS; SURFACE PROPERTIES; VOLATILE FATTY ACIDS; YEAST;

AMMONIUM COMPOUNDS;

AMMONIUM ACETATE; GLUTAMINASE; MAGNESIUM CHLORIDE; MALTOSE; MANNITOL; RNA 16S; SODIUM SULFATE;

ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BACTERIUM ISOLATION; BEEF; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; KLEBSIELLA OXYTOCA; NONHUMAN; PROCESS OPTIMIZATION; RNA SEQUENCE; SOIL MICROFLORA; YEAST;

KLEBSIELLA OXYTOCA;

EID: 79952683697     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12257-009-0187-8     Document Type: Article

References (21)

1. Rajeev Kumar, S. and M. Chandrasekaran (2003) Continuous production of L-glutaminase by an immobilized marine Pseudomonas sp BTMS-51 in a packed bed reactor. Proc. Biochem. 38: 1431-1436.
2. Nagendra Prabhu, G. and M. Chandrasekaran (1997) Impact of process parameters on L-glutaminase production by marine Vibrio costicola in solid state fermentation using polystyrene as an inert support. Proc. Biochem. 32: 285-289.
3. Ogawa, J. and S. Shimizu (1999) Microbial enzymes: New industrial applications from traditional screening methods. TIBTECH 17: 13-20.
4. Clarkson, K., B. Jones, R. Bott, B. Bower, G. Chotani, and T. Becker (2001) Enzymes: Screening, Expression, Design and Production. pp 315-352. In: M. R. Bedford and G. G. Partridge (eds.). Enzymes in Farm Animal Nutrition. CAB International,Oxon, UK.
5. Li, X. and L. Qin (2005) Metagenomics-based drug discovery and marine microbial diversity. TRENDS Biotechnol. 23: 539-543. (Pubitemid 41483769)
6. Greasham, R. L. (1983) Bioprocessing. Vol. 3, pp. 128-139. In: Rehm, H. J., G. Read, A. Puhler, and P. Stagler (eds.). Biotechnology. VCH Publishers Inc., NY.
7. Cochran, W. G. and G. M. Cox (1992) Experimental Designs. 4th Ed. Wiley, NY.
8. Cavalitto, S. F and C. F. Mignone (2007) Application of factorial and Doehlert designs for optimization of protopectinase production by a Geotrichum klebahnii strain. Proc. Biochem. 42: 175-179.
9. Kalil, S. J., F. Maugeri, and M. I. Rodrigues (2000) Response surface analysis and simulation as a tool for bioprocess design and optimization. Proc. Biochem. 35: 539-550.
10. Kulikova, A. I. and Iu. V. Galaev (1982) Metabolism of dicarboxylic amino acids and their amides in bacteria of the genus Klebsiella. Zh. Mikrobiol. Epidemiol. Immunobiol. 6: 44-47.
11. Moriguchi, M., K. Sakai, R. Tateyama, Y. Furuta, and M. Wakayama (1994) Isolation and characterization of salt-tolerant glutaminases from marine Micrococcus luteus K-3. J. Ferment. Bioeng. 77: 621-625.
12. Iyer, P. and R. Singhal (2008) Production of glutaminase (E.C.3.2.1.5) from Zygosaccharomyces rouxii: Statistical optimization using response surface methodology. Bioresour. Technol. 99: 4300-4307.
13. Curthoys, N. P. and R. F. Weiss (1974) Regulation of renal ammoniagenesis - subcellular localization of rat kidney glutaminase isoenzymes. J. Biol. Chem. 249: 3261-3266.
14. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
15. Tamer, M. and Y. Chisti (2001) Production and recovery of recombinant protease inhibitor α1-antitrypsin. Enz. Microbiol. Technol. 29: 611-620.
16. Roberts, J., J. S. Holcenberg, and W. C. Dolowy (1972) Isolation, crystallization and properties of Achromobacteraceae glutaminase-asparaginase with antitumor activity. J. Biol. Chem. 247: 84-90.
17. Omura, H., F. Sato, and K. Hayano (1983) A method for estimating L-glutaminase activity in soils. Soil Sci. P1 Nutr. (Tokyo) 29: 295-303.
18. Dharmaraj, K., N. Selvakumar, D. Chandramohan, and R. Natarajan (1977) L-glutaminase activity in marine sediments. Ind. J. Mar. Sci. 6: 168-170.
19. Galstyan, A. and E. G. Saakyan (1973) Determination of soil L-glutaminase activity. Dolk Akad Nauk SSSR 209: 1201-1202.
20. Santos, V. L., E. F. Araujo, E. G. Barros, and W. V. Guimaraes (1999) Fermentation of starch by Klebsiella oxytoca P2 containing plasmids with α-amylase and pullulanase genes. Biotech. Bioeng. 65: 673-676.
21. Cole, J. R., B. Chai, R. J. Farris, Q. Wang, A. S. Kulam-Syed-Mohideen, D. M. McGarrell, A. M. Bandela, E. Cardenas, G. M. Garrity, and J. M. Tiedje (2007) The ribosomal database project (RDP-II): introducing myRDP space and quality controlled public data. Nucleic Acids Res. 35 (Database issue): D169-D172. (Pubitemid 46056192)