Expression and purification of functional Clostridium perfringens alpha and epsilon toxins in Escherichia coli

Protein Expression and Purification

Volumn 77, Issue 2, 2011, Pages 207-213

  Zhao, Yao ^a   Kang, Lin ^a   Gao, Shan ^a   Zhou, Yang ^a   Su, Libo ^b   Xin, Wenwen ^a   Su, Yuxin ^a   Wang, Jinglin ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b JILIN UNIVERSITY   (China)

Author keywords

Alpha toxin; Bioactivity; Clostridium perfringens; Epsilon toxin; Expression; Purification

Indexed keywords

ALPHA TOXIN, CLOSTRIDIUM PERFRINGENS; BACTERIAL PROTEIN; BACTERIAL TOXIN; CALCIUM BINDING PROTEIN; HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE; PHOSPHOLIPASE C; THIOREDOXIN;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOASSAY; CELL LINE; CHEMISTRY; CLOSTRIDIUM PERFRINGENS; DOG; ERYTHROCYTE; ESCHERICHIA COLI; GEL CHROMATOGRAPHY; GENE VECTOR; GENETICS; HEMOLYSIS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SOLUBILITY;

ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; BIOLOGICAL ASSAY; CALCIUM-BINDING PROTEINS; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CLOSTRIDIUM PERFRINGENS; DOGS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROCYTES; ESCHERICHIA COLI; GENETIC VECTORS; HEMOLYSIS; HISTIDINE; MICE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS; SOLUBILITY; THIOREDOXINS; TYPE C PHOSPHOLIPASES;

CLOSTRIDIUM PERFRINGENS; ESCHERICHIA COLI;

EID: 79952627357     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.02.001     Document Type: Article

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