Abrin-a A chain expressed as soluble form in Escherichia coli from a PCR-synthesized gene is catalytically and functionally active

Biochimie

Volumn 86, Issue 4-5, 2004, Pages 327-333

  Wang, Li Chun ^a,b   Kang, Lin ^a   Hu, Ting Mao ^b   Wang, Jing Lin ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b INNER MONGOLIA UNIVERSITY   (China)

Author keywords

Abrin a A chain (ABRaA); N glycosylase; Soluble expression; Synthetic gene

Indexed keywords

ABRIN; ABRIN A; RIBOSOME RNA; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CANCER THERAPY; CELL FREE SYSTEM; CODON; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; EUKARYOTE; GENE EXPRESSION; HEPATOMA CELL; HUMAN; HUMAN CELL; LIVER; MOLECULAR CLONING; MYELOMA CELL; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; POLYMERASE CHAIN REACTION; PROTEIN SYNTHESIS; RABBIT; RIBOSOME; TUMOR CELL LINE;

ABRIN; ANIMALS; CARCINOMA, HEPATOCELLULAR; CATALYSIS; CELL-FREE SYSTEM; CYTOPLASM; ESCHERICHIA COLI; HUMANS; LIVER; MULTIPLE MYELOMA; N-GLYCOSYL HYDROLASES; POLYMERASE CHAIN REACTION; RATS; RECOMBINANT PROTEINS; RIBOSOMES; RNA, RIBOSOMAL, 28S;

ESCHERICHIA COLI; EUKARYOTA;

EID: 2942635587     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biochi.2004.03.006     Document Type: Article

References (28)

1. Lin J.Y., Lee T.C., Hu S.T., Tung T.C. Isoabrin of four isotoxic proteins and one agglutinin from jequirity bean (Abrus precatorius). J. Toxicol. 19:1981;41-45
2. Stripe F., Barbieri L., Matlelli M.G., Soria M., Lappi D.A. Ribosome-inactivating proteins: present status and future prospects. Biotechnology (New York). 10:1992;405-412
3. Endo Y., Mitsui K., Motizuki M., Tsurugi K. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins. J. Biol. Chem. 262:1987;5908-5912
4. Olsnes S., Sandvig K., Eiklid K. Properties and action mechanism of the toxic lectin modeccin: interaction with cell lines resistant to modeccin, abrin, and ricin. J. Supramol. Struct. 9:1978;15-25
5. Kuttan V.G., Kuttan R. Immunopotentiating activity of abrin, a lectin from Abrus precatorius. Indian J. Physiol. Pharmacol. 46:2002;69-77
6. Kreitman R.J., Pastan I. Immunotoxins for targeted cancer therapy. Adv. Drug Deliv. Rev. 31:1998;53-58
7. Wawrzynczak E.J., Zangemeister-Witter U., Waibel R., Henry R.V., Parnell G.D., Cumber A.J., Jones M., Stahel R.A. Molecular and biological properties of abrin A chain immunotoxin designed for therapy of human small cell lung cancer. Br. J. Cancer. 66:1992;361-366
8. Tsai L.C.H., Chen Y.L., Lee C.H., Chen H.M., Chang Z.N., Hung M.W. Growth suppression of human colorectal carcinoma in nude mice by monoclonal antibody C27-abrin A chain conjugate. Dis. Colon Rectum. 38:1995;1067-1074
9. Moriwaki S., Ohba H., Nakamura O. Biological activities of the lectin, abrin-a, against human lymphocytes and cultured leukemic cell lines. J. Hematother. Stem Cell Res. 9:2000;47-53
10. Ramnath V., Kuttan G., Kuttan R. Antitumour effect of abrin on transplanted tumors in mice. FEBS Lett. 522:2002;59-64
11. Wawrzynczak E.J., Cumber A.J., Henry R.V., May J., Newell D.R., Parnell G.D. Pharmacokinetics in the rat of a panel of immunotoxins made with abrin A chain, ricin A chain, gelonin, and momordin. Cancer Res. 50:1990;7519-7526
12. Hwang K.M., Foon K.A., Cheung P.H., Pearson J.W., Oldham R.K. Selective antitumor effect on L10 hepatocarcinoma cells of a potent immunoconjugate composed of the A chain of abrin and a monoclonal antibody to a hepatoma-associated antigen. Cancer Res. 44:1984;4578-4586
13. Forrester J.A., Mcintosh D.P., Cumber A.J., Parnell G.D., Ross W.C.J. Delivery of ricin and abrin A chains to human carcinoma cells in culture following linkage to monoclonal antibody LICR-LOND-Fib75. Cancer Drug Deliv. 1:1984;283-292
14. Blakey D.C., Wawrzynczak E.J., Stripe F., Thorpe P.E. Anti-tumor activity of a panel anti-Thy1.1 immunotoxins made with different ribosome-inactivating proteins. Bonavida B., Collier R.J. Membrane-Mediated Cytotoxicity. 1987;195-202 A.R. Liss Inc. New York
15. Sivam G., Pearson J.W., Bohn W., Oldham R.K., Sadoff J.C., Morgan A.C. Immunotoxins to human melanoma-associated antigen: comparison of gelonin with ricin and other A chain conjugates. Cancer Res. 47:1987;3169-3175
16. Thorpe P.E., Blakey D.C., Brown A.N.F., Knowles P.P., Knyba R.E., Wallace P.M. Comparison of two anti-Thy1.1 abrins A chain immunotoxins prepared with different cross-linking agents: anti-tumor effects, in vivo fate and tumor cell mutant. J. Natl. Cancer Inst. 79:1987;1101-1112
17. McGrath M.S., Hwang K.M., Caldwell S.E., Gaston I., Luk K.C., Wu P. GLQ223: an inhibitor of human immunodeficiency virus replication in acutely and chronically infected cells of lymphocyte and mononuclear phagocyte lineage. Proc. Natl. Acad. Sci. USA. 86:1989;2844-2848
18. Endo Y., Mitsui K., Motizuki M., Tsurugi K. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosome. J. Biol. Chem. 262:1987;5908-5912
19. Endo Y., Tsurugi K. RNA N-glycosidase activity of ricin A chain. J. Biol. Chem. 262:1987;8128-8130
20. Speeding G. Isolation and analysis of ribosome from prokaryotes, eukaryotes and organelles. Speeding G. Ribosomes and Protein Synthesis A Practical Approach. 1990;1-29 Oxford University Press, London
21. Thorpe P.E., Brown A.N., Ross W.C., Cumber A.J., Detre S.I., Edwards D.C. Cytotoxicity acquired by conjugation of an anti-Thy1.1 monoclonal antibody and the ribosome-inactivating protein, gelonin. Eur. J. Biochem. 116:1981;447-454
22. Marszal E., Suchova M., Konecny P., Scouten W.H. Study of cell-free protein synthesis in aqueous two-phase system. J. Mol. Recognit. 8:1995;151-156
23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65:1983;55-63
24. Hung C.H.H., Lee M.C.H., Lee T.C.H., Lin J.Y. Primary structure of three distinct isoabrins determined by cDNA sequencing conservation and significance. J. Mol. Biol. 229:1993;263-267
25. Evensen G., Mathiesen A., Sundan A. Direct molecular cloning and expression of two distinct abrin A-chains. J. Biol. Chem. 266:1991;6848-6852
26. Wood K.A., Lord J.M., Wawrzynczak E.J., Piatak M. Preproabrin: genomic cloning, characterization and the expression of the A-chain in Escherichia coli. Eur. J. Biochem. 198:1991;723-732
27. Hung C.H.H., Lee M.C.H., Chen J.K., Lin J.Y. Cloning and expression of three abrin A-chains and their mutants derived by site-specific mutagenesis in Escherichia coli. Eur. J. Biochem. 219:1994;83-87
28. Qing L.T., Qu X.L. Cloning, expression of the abrin-a A-chain in Escherichia coli and measurement of the biological activities in vitro. Acta Biochim. Biophys. Sin. 34:2002;405-410