Impact of pronase on flow cytometric crossmatch outcome

Human Immunology

Volumn 72, Issue 4, 2011, Pages 330-336

  Hetrick, Sarah J ^a   Schillinger, Karl P ^a   Zachary, Andrea A ^a   Jackson, Annette M ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Flow cytometric crossmatch; HLA expression; Pronase

Indexed keywords

HLA ANTIGEN; PRONASE;

ANTIGEN EXPRESSION; ARTICLE; BLOOD GROUP TYPING; CONTROLLED STUDY; FLOW CYTOMETRY; HLA MATCHING; HLA SYSTEM; HUMAN; LYMPHOCYTE; PRIORITY JOURNAL;

B-LYMPHOCYTES; FLOW CYTOMETRY; GENE EXPRESSION REGULATION; HISTOCOMPATIBILITY TESTING; HLA ANTIGENS; HUMANS; IMMUNOSUPPRESSIVE AGENTS; PRONASE; T-LYMPHOCYTES;

EID: 79952625738     PISSN: 01988859     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.humimm.2011.01.005     Document Type: Article

References (16)

1. American Society of Histocompatibility and Immunogenetics Membership Directory, 2010 Accessed 3 February 2010. http://www.ashi-hla.org/about/membership/directory.
2. O'Rourke R.W., Osorio R.W., Freise C.E., Lou C.D., Garovoy M.R., Bacchetti P., et al. Flow cytometry crossmatching as a predictor of acute rejection in sensitized recipients of cadaveric renal transplants. Clin Transpl 2000, 14:167-173.
3. Kerman R.H., Van Buren C.T., Lewis R.M., DeVera V., Baghadahsarian V., Gerolami K., et al. Improved graft survival for flow cytometry and antihuman globulin crossmatch-negative retransplant recipients. Transplantation 1990, 49:52-56.
4. Ilham M.A., Winkler S., Coates E., Rizzello A., Rees T., Asderakis A. Clinical significance of a positive flow crossmatch on the outcomes of cadaveric renal transplants. Transplant Proc 2008, 40:1839-1843.
5. Limaye S., O'Kelly P., Harmon G., O'Neill D., Dorman A., Walshe J., et al. Improved graft survival in highly sensitized patients undergoing renal transplantation after the introduction of a clinically validated flow cytometry crossmatch. Transplantation 2009, 87:1052-1056.
6. Karpinski M., Rush D., Jeffery J., Exner M., Regele H., Dancea S., et al. Flow cytometric crossmatching in primary renal transplant recipients with a negative anti-human globulin enhanced cytotoxicity crossmatch. J Am Soc Nephrol 2001, 12:2807-2814.
7. Lobo P.I., Spencer C.E., Isaacs R.B., McCullough C. Hyperacute renal allograft rejection from anti-HLA class 1 antibody to B cells-antibody detection by two color FCXM was possible only after using pronase-digested donor lymphocytes. Transpl Int 1997, 10:69-73.
8. Vaidya S., Cooper T., Stewart D., Gugliuzza K., Daller J., Bray R.A. Pronase improves detection of HLA antibodies in flow crossmatches. Transplant Proc 2001, 33:473-474.
9. Vaidya S., Cooper T.Y., Avandsalehi J., Barnes T., Brooks K., Hymel P., et al. Improved flow cytometric detection of HLA alloantibodies using pronase: potential implications in renal transplantation. Transplantation 2001, 71:422-428.
10. Agarwal A., Book B.K., Sidner R.A., Gebel H.M., Pescovitz M.D., Bray R.A. Removal of CD20 with pronase: implications for flow crossmatch in patients with rituximab. Hum Immunol 2002, 63:S103.
11. Lobo P.I., Spencer C.E., Stevenson W.C., McCullough C., Pruett T.L. The use of pronase-digested human leukocytes to improve specificity of the flow cytometric crossmatch. Transpl Int 1995, 8:472-480.
12. Bearden C.M., Agarwal A., Book B.K., Sidner R.A., Gebel H.M., Bray R.A., et al. Pronase treatment facilitates alloantibody flow cytometric and cytotoxic crossmatching in the presence of rituximab. Hum Immunol 2004, 65:803-809.
13. Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 1976, 5:9-15.
14. Hamrick C.W., Lebeck L.K. Flow cytometric T and B cell crossmatching. ASHI Laboratory Manual 2000, 1-5. ASHI, Mt. Laurel, NJ. 4th ed. A.B. Hahn, G.A. Land, R.M. Strothman (Eds.).
15. Zachary A.A., Sholander J.T., Houp J.A., Leffell M.S. Using real data for a virtual crossmatch. Hum Immunol 2009, 70:574-579.
16. Nadim E.A., Terasaki P.I., Nguyen A., Sasaki N., Morales-Buenrostro L.E., Saji H., et al. Epitopes of human leukocyte antigen class I antibodies found in sera of normal healthy males and cord blood. Hum Immunol 2009, 70:844-853.