Biochemical characterization of a new type of intracellular PHB depolymerase from Rhodospirillum rubrum with high hydrolytic activity on native PHB granules

Applied Microbiology and Biotechnology

Volumn 89, Issue 5, 2011, Pages 1487-1495

  Sznajder, Anna ^a   Jendrossek, Dieter ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

Denatured and artificial PHB; Extracellular PHB depolymerase native; Intracellular PHB depolymerase; PHA; PHB; PHB depolymerase products

Indexed keywords

DENATURED AND ARTIFICIAL PHB; DEPOLYMERASE; EXTRACELLULAR; PHA; PHB;

DENATURATION; ESCHERICHIA COLI; GENES; GRANULATION; HYDROLYSIS; MAGNESIUM; METAL IONS; PURIFICATION;

ENZYME ACTIVITY;

3 HYDROXYBUTYRIC ACID; BACTERIAL ENZYME; CALCIUM ION; CELL ENZYME; CYAN FLUORESCENT PROTEIN; EDETIC ACID; GENE PRODUCT; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; HYDROLASE; MAGNESIUM ION; MICROCRYSTALLINE CELLULOSE; POLY(3 HYDROXYBUTYRIC ACID); POLY(3 HYDROXYBUTYRIC ACID)DEPOLYMERASE; POLYGALACTURONASE; POLYHYDROXYALKANOIC ACID; RECOMBINANT ENZYME; TRYPSIN; UNCLASSIFIED DRUG;

BACTERIUM; BIOCHEMISTRY; ENZYME ACTIVITY; HYDROLYSIS; INHIBITION; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; BINDING AFFINITY; BIOCHEMICAL COMPOSITION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PURIFICATION; ESCHERICHIA COLI; EXTRACELLULAR MATRIX; FUSION GENE; GENE SEQUENCE; GENETIC DATABASE; HYDROLYSIS KINETICS; IN VIVO STUDY; INTRACELLULAR MEMBRANE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PURIFICATION; RECOMBINANT GENE; RHODOSPIRILLUM RUBRUM;

3-HYDROXYBUTYRIC ACID; CARBOXYLIC ESTER HYDROLASES; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; RECOMBINANT PROTEINS; RHODOSPIRILLUM RUBRUM; SUBSTRATE SPECIFICITY;

RHODOSPIRILLUM RUBRUM;

EID: 79952576127     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-011-3096-7     Document Type: Article

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