Production of serotonin by dual expression of tryptophan decarboxylase and tryptamine 5-hydroxylase in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 89, Issue 5, 2011, Pages 1387-1394

  Park, Sangkyu ^a   Kang, Kiyoon ^a   Lee, Shin Woo ^b   Ahn, Mi Jeong ^b   Bae, Jung Myung ^c   Back, Kyoungwhan ^a  

^a Chonnam National University   (South Korea)

^b Gyeongsang National University   (South Korea)

^c Korea University   (South Korea)

Author keywords

Dual expression; Escherichia coli; Serotonin; Tryptamine 5 hydroxylase; Tryptophan decarboxylase

Indexed keywords

A PLANTS; BIOGENIC AMINES; BIOSYNTHETIC GENE; CULTURE MEDIUM; CYTOCHROME P450; DUAL EXPRESSION; E. COLI; GLUTATHIONE S-TRANSFERASES; HETEROLOGOUS EXPRESSION; HYDROXYLASES; INDUCTION TEMPERATURES; N-TERMINALS; OPTIMUM TEMPERATURE; SEROTONIN; SOLUBLE EXPRESSION; TRYPTAMINES; TRYPTOPHAN DECARBOXYLASE;

AMINO ACIDS; CELL CULTURE; ENZYMES; ESCHERICHIA COLI; LOW TEMPERATURE PRODUCTION;

GENE EXPRESSION;

ALCOHOL; AROMATIC LEVO AMINO ACID DECARBOXYLASE; BACTERIAL ENZYME; CYTOCHROME P450; GLUTATHIONE TRANSFERASE; OXYGENASE; RECOMBINANT PROTEIN; SEROTONIN; TRYPTAMINE 5 HYDROXYLASE; UNCLASSIFIED DRUG;

AMINO ACID; BACTERIUM; BIOGENIC MATERIAL; ENZYME ACTIVITY; GENE;

AMINO TERMINAL SEQUENCE; ARTICLE; BIOSYNTHESIS; CELL FRACTIONATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE DELETION; GENE EXPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PLANT GENE; PROTEIN EXPRESSION; PROTEIN TRANSPORT; RECOMBINANT GENE; SEROTONIN RELEASE; TEMPERATURE; TEMPERATURE SENSITIVITY;

AROMATIC-L-AMINO-ACID DECARBOXYLASES; CULTURE MEDIA; CYTOCHROME P-450 ENZYME SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; MUTANT PROTEINS; RECOMBINANT FUSION PROTEINS; SEROTONIN;

ESCHERICHIA COLI;

EID: 79952575144     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2994-4     Document Type: Article

References (27)

1. Anstey ML, Rogers SM, Ott SR, Burrows M, Simpson SJ (2009) Serotonin mediates behavioral gregarization underlying swarm formation in desert locusts. Science 323:627-630
2. Back K, Yin S, Chappell J (1994) Expression of a plant sesquiterpene cyclase gene in Escherichia coli. Arch Biochem Biophys 315:527-532 (Pubitemid 24381577)
3. Barnes HJ, Arlotto MP, Waterman MR (1991) Expression and enzymatic activity of recombinant cytochrome P450 17α- hydroxylase in Escherichia coli. Proc Natl Acad Sci USA 88:5597-5601 (Pubitemid 21914792)
4. Cabrita LD, Dai W, Bottomley SP (2005) A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol 6:12
5. Fujiwara T, Maisonneuve S, Isshiki M, Mizutani M, Chen L, Wong HL, Kawasaki T, Shimamoto K (2010) Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice. J Biol Chem 285:11308-11313
6. Halkier BA, Nielsen HL, Koch B, Møller L (1995) Purification and characterization of recombinant cytochrome P450TYR expressed at high levels in Escherichia coli. Arch Biochem Biophys 322:369-377
7. Hong SM, Yamashita J, Mitsunobu H, Uchino K, Kobayashi I, Sezutsu H, Tamura T, Nakajima H, Miyagawa Y, Lee JM, Mon H, Miyata Y, Kawaguchi Y, Kusakabe T (2010) Efficient soluble protein production on transgenic silkworms expressing cytoplasmic chaperones. Appl Microbiol Biotechnol 87:2147-2156
8. Hotze M, Schröder G, Schröder J (1995) Cinnamate 4-hydroxylase from Catharanthus roseus, and a strategy for the functional expression of plant cytochrome P450 protein as translational fusion with P450 reductase in Escherichia coli. FEBS Lett 374:345-350
9. Ishihara A, Hashimoto Y, Tanaka C, Dubouzet JG, Nakao T, Matsuda F, Nishioka T, Miyagawa H, Wakasa K (2008) The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production. Plant J 54:481-495 (Pubitemid 351585496)
10. Jenkins CM, Waterman MR (1998) NADPH-flavodoxin reductase and flavodoxin from Escherichia coli: characteristics as a soluble microsomal P450 reductase. Biochemistry 37:6106-6113 (Pubitemid 28196767)
11. Kang K, Kim YS, Park S, Back K (2009a) Senescence-induced serotonin biosynthesis and its role in delaying senescence in rice leaves. Plant Physiol 150:1380-1393
12. Kang K, Park S, Kim YS, Lee S, Back K (2009b) Biosynthesis and biotechnological production of serotonin derivatives. Appl Microbiol Biotechnol 83:27-34
13. LaVallie ER, Lu Z, Diblasio-Smith EA, Collins-Racie LA, McCoy JM (2000) Thioredoxin as a fusion partner for production of soluble recombinant proteins in Escherichia coli. Methods Enzymol 326:322-340
14. Leonard E, Yan Y, Koffas MAG (2006) Functional expression of a P450 flavonoid hydroxylase for the biosynthesis of plant-specific hydroxylated flavonols in Escherichia coli. Metab Eng 8:172-181 (Pubitemid 43356399)
15. Lesurtel M, Graf R, Aleil B, Walther DJ, Tian Y, Jochum W, Gachet C, Bader M, Clavien PA (2006) Platelet-derived serotonin mediates liver regeneration. Science 312:104-107
16. Mohammad-Zadeh LF, Moses L, Gwaltney-Brant SM (2008) Serotonin: a review. J Vet Pharmacol Therap 31:187-199 (Pubitemid 351677387)
17. Muñoz-Castañeda JR, Montilla P, Padillo FJ, Bujalance I, Muñoz MC, Muntané TI (2006) Role of serotonin in cerebral oxidative stress in rats. Acta Neurobiol Exp 66:1-6
18. Nelson DR, Schuler MA, Paquette SM, Werck-Reichhart D, Bak SØren (2004) Comparative genomics of rice and Arabidopsis. Analysis of 727 cytochrome P450 genes and pseudogenes from a monocot and a dicot. Plant Physiol 135:756-772
19. O'Keefe DP, Leto KJ (1989) Cytochrome P450 from the mesocarp of avocado (Persea americana). Plant Physiol 89:1141-1149
20. Oeda K, Sakaki T, Ohkawa H (1985) Expression of rat liver cytochrome P-450MC cDNA in Saccharomyces cerevisiae. DNA 4:203-210 (Pubitemid 15027392)
21. Ro DK, Paradise EM, Quellet M, Fisher KJ, Newman KL, Ndungu JM, Ho KA, Eachus RA, Ham TS, Kirby J, Chang MCY, Withers ST, Shiba Y, Sarpong R, Keasling JD (2006) Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 440:940-943
22. Roshchina VV (2001) Neurotransmitters in plant life. Science Publishers, Enfield, pp 4-81
23. Schein CH, Noteborn MHM (1988) Formation of soluble recombinant proteins in Escherichia coli is favored by lower growth temperature. Nature Biotech 6:291-294
24. Schröder G, Unterbusch E, Kaltenbach M, Schmidt J, Strack D, De Luca V, Schröder J (1999) Light-induced cytochrome P450- dependent enzyme in indole alkaloid biosynthesis: tabersonine 16-hydroxylase. FEBS Lett 458:97-102
25. Semba H, Ichige E, Imanaka T, Atomi H, Aoyagi H (2008) Efficient production of active form of recombinant cassava hydroxynitrile lyase using Escherichia coli in low-temperature culture. Appl Microbiol Biotechnol 79:563-569
26. Steczko J, Donoho GA, Dixon JE, Sugimoto T, Axelrod B (1991) Effect of ethanol and low-temperature culture on expression of soybean lipoxygenase L-1 in Escherichia coli. Protein Expr Purif 2:221-227
27. Tan DX, Manchester LC, Terron MP, Flores LJ, Reiter RJ (2007) One molecule, many derivatives: a never-ending interaction of melatonin with reactive oxygen and nitrogen species. J Pineal Res 42:28-42 (Pubitemid 44924323)