β-galactosidase from Kluyveromyces lactis cell disruption and enzyme immobilization using a cellulose-gelatin carrier system

Process Biochemistry

Volumn 39, Issue 6, 2004, Pages 705-711

  Numanoǧlu, Yasemin ^a   Sungur, Sibel ^a  

^a ANKARA UNIVERSITY   (Turkey)

Author keywords

Disruption; Gelatin; Immobilization; Kluyveromyces lactis; Yeast; Galactosidase

Indexed keywords

KLUYVEROMYCES; KLUYVEROMYCES LACTIS;

EID: 79952573749     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(03)00183-3     Document Type: Article

References (23)

1. Ohmiya K., Ohashi H., Kobayashi T., Shimizu S. Hydrolysis of lactose by immobilized microorganisms. Appl. Environ. Microbiol. 33:(1):1977;137-146.
2. Kula M., Schutte H. Purification of protein and the disruption of microbial cells. Biotechnol. Prog. 3:1987;31-42.
3. Asenjo J.A., Patrick I. Large-scale protein purification. Harris E.L.V., Angel S. Protein purification applications a practical approach. 1990;12 Oxford University Press, Oxford.
4. 4 and triton X100. Biotechnol. Prog. 17:(3):2001;490-494.
5. Fonseca L.P., Cabral J.M.S. Penicillin acylase release from Escherichia coli cells by mechanical cell disruption and permeabilization. J. Chem. Technol. Biotechnol. 77:(2):2002;159-167.
6. Irazoqui G., Villarino A., Batista-Viera F., Brena B.M. Activity and stability of Escherichia coli beta-galactosidase in cosolvent systems. Biotechnol. Tech. 12:(12):1998;885-888.
7. Guneva V., Galutzov B., Eynard N., Teissie J. Electroinduced extraction of β-galactosidase from Kluyveromyces lactis. Appl. Microbiol. Biotechnol. 56:2001;411-413.
8. Ismail S.A., Mabrouk S.S., Mahoney R.R. Purification and characterization of β-galactosidase from Mucor pusillus. J. Food Biochem. 21:1997;145-162.
9. Karel S.F., Libicki S.B., Robertson C.R. The immobilization of whole cells: engineering principles. Chem. Eng. Sci. 40:1985;1321-1354.
10. Mosbach K. Methods in enzymology. 136:1987;351-540 Academic Press, Orlando, FL, USA.
11. Gekas V., Lopez-Leiva M. Hydrolysis of lactose: a literature review. Process Biochem. 20:1985;2-11.
12. Mahoney R.R., Whitaker R. Purification and physicochemical properties of β-galactosidase from Kluyveromyces fragilis. J. Food Sci. 43:1978;584-591.
13. Stred'ansky M., Tomaska M., Sturdik E., Kremnicky L. Optimization of β-galactosidase extraction from Kluyveromyces marxianus. Enzyme Microb. Technol. 15:1993;1063-1065.
14. Sungur S., Akbulut U. Immobilization of β-galactasidase onto gelatin by glutaraldehyde and chromium (III) acetate. J. Chem. Tech. Biotechnol. 59:1994;303-306.
15. Sungur S., Yildirim Ö. Batch and continuous hydrolysis of lactose using β-galactosidase immobilized on gelatine-CMC. Polym.-Plast. Technol. Eng. 38:(4):1999;19-27.
16. Becerra M., Gonzalez Siso M.I. Yeast β-galactosidase in solid-state fermentations. Enzyme Microb. Technol. 19:1996;39-44.
17. Castillo E., Rodriguez M., Casas L., Quintero R., Lopez-Munguia A. Design of two immobilized catalysts by entrapment on gelatin: internal diffusion aspects. Enzyme Microb. Technol. 13:1991;127-133.
18. Champluvier B., Marchal F., Rouxhet P.G. Immobilization of lactase in yeast cells retained in a glass wool matrix. Enzyme Microb. Technol. 11:1989;422-430.
19. Papayannakos N., Markas G., Kekos D. Studies on modeling and simulation of lactose hydrolysis by free and immobilized β-galactosidase from Aspergillus niger. Chem. Eng. J. 52:1993;B1-B12.
20. Price N.C. Techniques for enzyme extraction. Eisenthal R., Danson J.M. Enzyme assays a practical approach. 1992;Oxford University Press, Oxford.
21. Sigma Chemical Co., Technical Bulletin No. 510, 1983.
22. Sungur S., Elçin M., Akbulut U. Immobilization of urease into carboxymethylcellulose-gelatine system. Macromol. Rep. A29:(3):1992;251-265.
23. Furlan S.A., Schneider A.L.S., Merkle R., Carvalho-Jonas M.F., Jones R. Optimization of pH, temperature and inoculum ratio for the production of β-D-galactosidase by Kluyveromyces marxianus using a lactose-free medium. Acta Biotechnol. 21:(1):2001;57-64.