Fluorescent Site-Specific Labeling of Escherichia coli Expressed Proteins with Sfp Phosphopantetheinyl Transferase

Methods in Molecular Biology

Volumn 705, Issue , 2011, Pages 295-307

  Zhang, Aihua ^a   Sun, Luo ^a   Buswell, John ^a   Considine, Nancy ^a   Ghosh, Inca ^a   Masharina, Anastasiya ^a   Noren, Christopher ^a   Xu, Ming Qun ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

ACP synthase; ACP tag; fluorescent labeling; Protein labeling; S6 tag; Sfp synthase; ybbR

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

BACTERIAL PROTEIN; E3 14.7K PROTEIN, ADENOVIRUS; FLUORESCENT DYE; HYBRID PROTEIN; PHOSPHOPANTETHEINYL TRANSFERASE; PHOSPHOTRANSFERASE; VIRUS PROTEIN;

BIOSYNTHESIS; CHEMISTRY; ESCHERICHIA COLI; GENETICS; METABOLISM; REVIEW;

ADENOVIRUS E3 PROTEINS; BACTERIAL PROTEINS; ESCHERICHIA COLI; FLUORESCENT DYES; RECOMBINANT FUSION PROTEINS; TRANSFERASES (OTHER SUBSTITUTED PHOSPHATE GROUPS);

EID: 79952483265     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-967-3_18     Document Type: Chapter

References (17)

1. Wang, H., Chen, X. (2008) Site-specifically modified fusion proteins for molecular imaging. Front Biosci 13, 1716–1732.
2. Chen I., Ting A. Y. (2005) Site-specific labeling of proteins with small molecules in live cells. Curr Opin Biotechnol 16, 35–40.
3. Tsien, R. Y. (1998) The green fluorescent protein. Annu Rev Biochem 67, 509–544.
4. Griffin B. A., Adams, S. R., Tsien, R. Y. (1998) Specific covalent labeling of recombinant protein molecules inside live cells. Science 10, 269–272.
5. Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., Johnsson, K. (2002) A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21, 86–89.
6. Yin, J., Lin, A. J., Golan, D. E., Walsh, C. T. (2006) Site-specific protein labeling by Sfp phosphopantetheinyl transferase. Nat Protoc 1, 280–285.
7. Zhou, Z., Cironi, P., Lin, A. J., Xu, Y., Hrvatin, S., Golan, D. E., et al. (2007) Genetically encoded short peptide tags for Orthogonal protein labeling by Sfp and Acps phosphopantetheinyl transferases. ACS Chem Biol 2, 337–346.
8. Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M., Beaufils, F., Johnsson, K. (2008) An engineered protein tag for multiprotein labeling in living cells. Chem Biol 15, 128–136.
9. Meyer, B. H., Segura, J., Martinez, K. L., Hovius, R., George, N., Johnsson, K., Vogel, H. (2006) FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells. Proc Natl Acad Sci USA 103, 2138–2143.
10. Yin, J., Lin, A. J., Buckett, P. D., Wessling-Resnick, M., Golan, D. E., Walsh, C. T. (2005) Single-cell FRET imaging of trans-ferrin receptor trafficking dynamics by Sfp-catalyzed, site-specific protein labeling. Chem Biol 12, 999–1006.
11. Jacquier, V., Prummer, M., Segura, J.-M., Pick, H., Vogel, H. (2006) Visualizing odorant receptor trafficking in living cells down to the single-molecule level. Proc Natl Acad Sci USA 103, 14325–14330.
12. Yin, J., Straight, P. D., McLoughlin, S. M., Zhou, Z., Lin, A. J., Golan, D. E., et al. (2005) Genetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase. Proc Natl Acad Sci USA 102, 15815–15820.
13. Schneider-Brachert, W., Tchikov, V., Merkel, O., Jakob, M., Hallas, C., et al. (2006) Inhibition of TNF receptor 1 internalization by adenovirus 14.7 K as a novel immune escape mechanism. J Clin Invest 116, 2901–2913.
14. Ferrandon, S., Sterzenbach, T., Mersha, F., Xu, M.-Q. (2003) A single surface tryp-tophan in the chitin-binding domain from Bacillus circulans chitinase A1 plays a pivotal role in binding chitin. Biochim Biophys Acta 1621, 31–40.
15. Evans, T. C., Jr., Benner, J., Xu, M.-Q. (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci 7, 2256–2264.
16. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of micro-gram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72, 248–254.
17. Spector, D., Goldman, R. (eds.) (2004). Live Cell Imaging: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.