MFI ratio estimation of ZAP-70 in B-CLL by flow cytometry can be improved by considering the isotype-matched antibody signal

International Journal of Laboratory Hematology

Volumn 33, Issue 2, 2011, Pages 194-200

  Marquez, M E ^a   Deglesne, P A ^a   Suarez, G ^a   Romano, E ^a  

^a INSTITUTO VENEZOLANO DE INVESTIGACIONES CIENTÍFICAS   (Venezuela)

Author keywords

Chronic lymphocytic leukemia; Flow cytometry; IgVH mutational status; Mean fluorescent index; ZAP 70

Indexed keywords

IMMUNOGLOBULIN HEAVY CHAIN; PROTEIN KINASE ZAP 70;

ARTICLE; B LYMPHOCYTE; CHRONIC LYMPHATIC LEUKEMIA; FLOW CYTOMETRY; GENE MUTATION; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION;

FLOW CYTOMETRY; HUMANS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN ISOTYPES; IMMUNOGLOBULIN VARIABLE REGION; LEUKEMIA, LYMPHOCYTIC, CHRONIC, B-CELL; MUTATION; TUMOR MARKERS, BIOLOGICAL; ZAP-70 PROTEIN-TYROSINE KINASE;

EID: 79952460520     PISSN: 17515521     EISSN: 1751553X     Source Type: Journal    
DOI: 10.1111/j.1751-553X.2010.01263.x     Document Type: Article

References (23)

1. Bakke A.C., Purtzer Z., Leis J. & Huang J. (2006) A robust ratio metric method for analysis of Zap-70 expression in chronic lymphocytic leukemia (CLL). Cytometry Part B - Clinical Cytometry 70, 227-234.
2. Bekkema R., Tadema A., Daenen S.M.G.J., Kluin-Nelemans H.C. & Mulder A.B. (2008) An improved flow cytometric method using FACS lysing solution for measurement of ZAP-70 expression in B-cell chronic lymphocytic leukemia. Cytometry Part B - Clinical Cytometry 74, 40-44.
3. H mutational status in chronic lymphocytic leukemia. American Journal of Clinical Pathology 127, 182-191.
4. Crespo M., Bosch F., Villamor N., Bellosillo B., Colomer D., Rozman M., Marce S., Lopez-Guillermo A., Campo E. & Montserrat E. (2003) ZAP-70 expression as a surrogate for immunoglobulin-variable-region mutations in chronic lymphocytic leukemia. New England Journal of Medicine 348, 1764-1775.
5. Damle R.N., Wasil T., Fais F., Ghiotto F., Valetto A., Allen S.L., Buchbinder A., Budman D., Dittmar K., Kolitz J., Lichtman S.M., Schulman P., Vinciguerra V.P., Rai K.R., Ferrarini M. & Chiorazzi N. (1999) Ig V gene mutation status and CD38 expression as novel prognostic indicators in chronic lymphocytic leukemia. Blood 94, 1840-1847.
6. Deglesne P.A., Chevallier N., Letestu R., Baran-Marszak F., Beitar T., Salanoubat C., Sanhes L., Nataf J., Roger C., Varin-Blank N. & Ajchenbaum-Cymbalista F. (2006) Survival response to B-cell receptor ligation is restricted to progressive chronic lymphocytic leukemia cells irrespective of Zap70 expression. Cancer Research 66, 7158-7166.
7. Durig J., Naschar M., Schmucker U., Renzing-Kuhler K., Holter T., Hutman A. & Duhrsen U. (2002) CD38 expression is an important prognostic marker in chronic lymphocytic leukaemia. Leukemia 16, 30-35.
8. Durig J., Nuckel H., Cremer M., Fuhrer A., Halfmeyer K., Fandrey J., Moroy T., Klein-Hitpass L. & Duhrsen U. (2003) ZAP-70 expression is a prognostic factor in chronic lymphocytic leukemia. Leukemia 17, 2426-2434.
9. Gachard N., Salviat A., Boutet C., Arnoulet C., Durrieu F., Lenormand B., Lepretre S., Olschwang S., Jardin F., Lafage-Pochitaloff M., Penther D., Sainty D., Reminieras L., Feuillard J. & Bene M.C. (2008) Multicenter study of ZAP-70 expression in patients with B-cell chronic lymphocytic leukemia using an optimized flow cytometry method. Haematologica 93, 215-223.
10. Gibbs G., Bromidge T., Howe D., Hopkins J. & Johnson S. (2005) Comparison of flow cytometric methods for the measurement of ZAP-70 expression in a routine diagnostic laboratory. Clinical and Laboratory Haematology 27, 258-266.
11. Hamblin T.J., Davis Z., Gardiner A., Oscier D.G. & Stevenson F.K. (1999) Unmutated Ig V(H) genes are associated with a more aggressive form of chronic lymphocytic leukemia. Blood 94, 1848-1854.
12. Kay S., Herishanu Y., Rogowski O., Baron S., Naparsek E., Polliack A. & Deutsh V.R. (2006) Quantitative flow cytometry of ZAP-70 levels in chronic lymphocytic leukemia using molecules of equivalent soluble fluorochrome. Cytometry Part B - Clinical Cytometry 70, 218-226.
13. H mutation status and cytogenetics in 1229 patients with chronic lymphocytic leukemia. Cytometry Part B - Clinical Cytometry 76, 385-393.
14. H mutation status, CD38 expression level, genomic aberrations, and survival in chronic lymphocytic leukemia. Blood 100, 1410-1416.
15. Le Garff-Tavernier M., Ticchioni M., Brissard M., Salmon C., Raynaud S., Davi F., Bernard A., Merle-Béral H., Ajchenbaum-Cymbalista F. & Letestu R. (2006) National standardization of ZAP-70 determination by flow cytometry: the French experience. Cytometry Part B - Clinical Cytometry 72B, 103-108.
16. Letestu R., Rawstron A., Ghia P., Villamor N., Boeckx N., Boettcher S., Buhl A.M., Duerig J., Ibbotson R., Kroeber A., Langerak A., Le Garff-Tavernier M., Mockridge I., Morilla A., Padmore R., Rassenti L., Ritgen M., Shehata M., Smolewski P., Staib P., Ticchioni M., Walker C. & Ajchenbaum-Cymbalista F. (2008) Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: a multicentric international harmonization process. Cytometry Part B - Clinical Cytometry 74, 259.
17. Maloum K., Davi F., Merle-Beral H., Pritsch O., Magnac C., Vuillier F., Dighiero G., Troussard X., Mauro F.F. & Benichou J. (2000) Expression of unmutated V(H) genes is a detrimental prognostic factor in chronic lymphocytic leukemia [6]. Blood 96, 377-379.
18. Orchard J.A., Ibbotson R.E., Davis Z., Wiestner A., Rosenwald A., Thomas P.W., Hamblin T.J., Staudt L.M. & Oscier D.G. (2004) ZAP-70 expression and prognosis in chronic lymphocytic leukaemia. Lancet 363, 105-111.
19. Rassenti L.Z., Huynh L., Toy T.L., Chen L., Keating M.J., Gribben J.G., Neuberg D.S., Flinn I.W., Rai K.R., Byrd J.C., Kay N.E., Greaves A., Weiss A. & Kipps T.J. (2004) ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. New England Journal of Medicine 351, 893-901.
20. Rosenwald A., Alizadeh A.A., Widhopf G., Simon R., Davis R.E., Yu X., Yang L., Pickeral O.K., Rassenti L.Z., Powell J., Botstein D., Byrd J.C., Grever M.R., Cheson B.D., Chiorazzi N., Wilson W.H., Kipps T.J., Brown P.O. & Staudt L.M. (2001) Relation of gene expression phenotype to immunoglobulin mutation genotype in B cell chronic lymphocytic leukemia. Journal of Experimental Medicine 194, 1639-1647.
21. Shankey T.V., Forman M., Scibelli P., Cobb J., Smith C.M., Mills R., Holdaway K., Bernal-Hoyos E., Van Der Heiden M., Popma J. & Keeney M. (2006) An optimized whole blood method for flow cytometric measurement of ZAP-70 protein expression in chronic lymphocytic leukemia. Cytometry Part B - Clinical Cytometry 70, 259-269.
22. Wiestner A., Rosenwald A., Barry T.S., Wright G., Davis R.E., Henrickson S.E., Zhao H., Ibbotson R.E., Orchard J.A., Davis Z., Stetler-Stevenson M., Raffeld M., Arthur D.C., Marti G.E., Wilson W.H., Hamblin T.J., Oscier D.G. & Staudt L.M. (2003) ZAP-70 expression identifies a chronic lymphocytic leukemia subtype with unmutated immunoglobulin genes, inferior clinical outcome, and distinct gene expression profile. Blood 101, 4944-4951.
23. H mutations. Cytometry Part B - Clinical Cytometry 70, 284-292.