Glutathione modulates the toxicity of, but is not a biologically relevant reductant for, the Pseudomonas aeruginosa redox toxin pyocyanin

Free Radical Biology and Medicine

Volumn 50, Issue 8, 2011, Pages 971-977

  Muller, Michael ^a  

^a UNIVERSITY OF WESTERN SYDNEY   (Australia)

Author keywords

CF; cystic fibrosis; electron paramagnetic resonance; EPR; glutathione; glutathione disulfide; GSH; GSSG; HDF; human dermal fibroblast; N acetylcysteine; NAC; NADPH; nicotinamide adenine dinucleotide phosphate (reduced); PD; population doubling; reactive oxygen species; ROS

Indexed keywords

ACETYLCYSTEINE; CYSTEINE; GLUTATHIONE; HYDROGEN PEROXIDE; PYOCYANINE; REACTIVE OXYGEN METABOLITE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ANAEROBIC BACTERIUM; ARTICLE; CELL AGING; CELL PROTECTION; CONTROLLED STUDY; CYTOTOXICITY; DRUG OXIDATION; HUMAN; HUMAN CELL; HUMAN TISSUE; MODULATION; PRIORITY JOURNAL; PROTEIN DEPLETION; PSEUDOMONAS AERUGINOSA; REDOX STRESS; SKIN FIBROBLAST; SPECTROPHOTOMETRY;

ACETYLCYSTEINE; CELL DIVISION; CELLS, CULTURED; CYSTEINE; ELECTRON SPIN RESONANCE SPECTROSCOPY; GLUTATHIONE; HUMANS; NADP; OXIDATION-REDUCTION; PSEUDOMONAS AERUGINOSA; PYOCYANINE; REACTIVE OXYGEN SPECIES;

BACTERIA (MICROORGANISMS); MAMMALIA; PSEUDOMONAS AERUGINOSA;

EID: 79952441104     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2011.01.012     Document Type: Article

References (37)

1. A. Meister, and M.E. Anderson Glutathione Annu. Rev. Biochem. 52 1983 711 760 (Pubitemid 13030692)
2. R.E. Shackelford, A.N. Heinloth, S.C. Heard, and R.S. Paules Cellular and molecular targets of S-glutathiolation Antioxid. Redox Signaling 7 2005 940 950
3. I. Rahman, S.K. Biswas, L.A. Jimenez, M. Torres, and H.J. Forman Glutathione, stress responses, and redox signaling in lung inflammation Antioxid. Redox Signaling 7 2005 42 59 (Pubitemid 40187924)
4. R. Liu M.; Gaston Pravia, K. A. Oxidative stress and glutathione in TGF-beta-mediated fibrogenesis Free Radic. Biol. Med. 48 2010 1 15
5. L. Chaiswing, and T.D. Oberley Extracellular/microenvironmental redox state Antioxid. Redox Signaling 13 2010 449 465
6. C.C. Caldwell, Y. Chen, H.S. Goetzmann, Y. Hao, M.T. Borchers, D.J. Hassett, L.R. Young, D. Mavrodi, L. Thomashow, and G.W. Lau Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis Am. J. Pathol. 175 2009 2473 2488
7. G.A. James, E. Swogger, R. Wolcott, E. deLancey Pulcini, P. Secor, J. Sestrich, J.W. Costerton, and P.S. Stewart Biofilms in chronic wounds Wound Rep. Reg. 16 2008 37 44
8. K. Gjødsbøl, J.J. Christensen, T. Karlsmark, B. Jørgensen, B.M. Klein, and K.A. Krogfelt Multiple bacterial species reside in chronic wounds: a longitudinal study Int. Wound J. 3 2006 225 231
9. R. Wilson, D.A. Sykes, D. Watson, A. Rutman, G.W. Taylor, and P.J. Cole Measurement of Pseudomonas aeruginosa phenazine pigments in sputum and assessment of their contribution to sputum sol toxicity for respiratory epithelium Infect. Immun. 56 1988 2515 2517
10. M. Muller, Z. Li, and P.K.M. Maitz Pseudomonas pyocyanin inhibits wound repair by inducing premature cellular senescence: role for p38 mitogen-activated protein kinase Burns 35 2009 500 508
11. M. Muller Pyocyanin induces oxidative stress in human endothelial cells and modulates the glutathione redox cycle Free Radic. Biol. Med. 33 2002 1527 1533 (Pubitemid 35351596)
12. L. Allen, D.H. Dockrell, T. Pattery, D.G. Lee, P. Cornelis, P.G. Hellewell, and M.K.B. Whyte Pyocyanin production by Pseudomonas aeruginosa induces neutrophil apoptosis and impairs neutrophil-mediated host defenses in vivo J. Immunol. 174 2005 3643 3649 (Pubitemid 40354074)
13. M. Muller Premature cellular senescence induced by pyocyanin, a redox active Pseudomonas aeruginosa toxin Free Radic. Biol. Med. 41 2006 1670 1677 (Pubitemid 44634242)
14. P.K. Müller, K. Krohn, and P.F. Muhlradt Effects of pyocyanine, a phenazine dye from Pseudomonas aeruginosa, on oxidative burst and bacterial killing in human neutrophils Infect. Immun. 57 1989 2591 2596 (Pubitemid 19212585)
15. Y.Q. OMalley, K.J. Reszka, D.R. Spitz, G.M. Denning, and B.E. Britigan Pseudomonas aeruginosa pyocyanin directly oxidizes glutathione and decreases its levels in airway epithelial cells Am. J. Physiol. Lung Cell. Mol. Physiol. 287 2004 L94 L103
16. G.W. Lau, D.J. Hassett, H. Ran, and F. Kong The role of pyocyanin in Pseudomonas aeruginosa infection Trends Mol. Med. 10 2004 599 606 (Pubitemid 39573151)
17. B. Rada, K. Lekstrom, S. Damian, C. Dupuy, and T. Leto The Pseudomonas toxin pyocyanin inhibits the dual oxidase-based antimicrobial system as it imposes oxidative stress on airway epithelial cells J. Immunol. 181 2008 4883 4893
18. G.Y. Liu, and V. Nizet Color me bad: microbial pigments as virulence factors Trends Microbiol. 17 2009 406 413
19. C. Jacob, V. Jamier, and L.A. Ba Redox active secondary metabolites Curr. Opin. Chem. Biol. 15 2010 1 7
20. - transport in human bronchial epithelial cells Free Radic. Biol. Med. 45 2008 1653 1662
21. J.H. Roum, Z. Borok, N.G. McElvaney, G.J. Grimes, A.D. Bokser, R. Buhl, and R.G. Crystal Glutathione aerosol suppresses lung epithelial surface inflammatory cell-derived oxidants in cystic fibrosis J. Appl. Physiol. 87 1999 438 443 (Pubitemid 29330711)
22. A. Visca, C.T. Bishop, S.C. Hilton, and V.M. Hudson Improvement in clinical markers in CF patients using a reduced glutathione regimen: an uncontrolled, observational study J. Cystic Fibrosis 7 2008 433 436
23. R. Tirouvanziam, C.K. Conrad, T. Bottiglieri, L.A. Herzenberg, R.B. Moss, and L.A. Herzenberg High-dose oral N-acetylcysteine, a glutathione prodrug, modulates inflammation in cystic fibrosis Proc. Natl Acad. Sci. USA 103 2006 4628 4633
24. M. Knight, P.E. Hartman, Z. Hartman, and V.M. Young A new method of preparation of pyocyanin and demonstration of an unusual bacterial sensitivity Anal. Biochem. 95 1979 19 23 (Pubitemid 9194265)
25. G. Davis, and P.J. Thornalley Free radical production from the aerobic oxidation of reduced pyridine nucleotides catalysed by phenazine derivatives Biochim. Biophys. Acta 724 1983 456 464
26. O.W. Griffith Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine Anal. Biochem. 106 1980 207 212 (Pubitemid 11246585)
27. H.P. Misra Generation of superoxide free radical during the autoxidation of thiols J. Biol. Chem. 249 1974 2151 2155
28. G. Saez, P.J. Thornalley, H.A. Hill, R. Hems, and J.V. Bannister The production of free radicals during the autoxidation of cysteine and their effects of isolated rat hepatocytes Biochim. Biophys. Acta 719 1982 24 31 (Pubitemid 13223068)
29. R. Stevens, L. Stevens, and N.C. Price The stabilities of various thiol compounds used in protein purifications Biochem. Educ. 11 1983 70
30. M. Muller Scavenging of neutrophil-derived superoxide anion by 1-hydroxyphenazine, a phenazine derivative associated with chronic Pseudomonas aeruginosa infection: relevance to cystic fibrosis Biochim. Biophys. Acta 1272 1995 185 189 (Pubitemid 26009855)
31. E. Friedheim, and L. Michaelis Potentiometric study of pyocyanine J. Biol. Chem. 91 1931 355 368
32. L.A. Schneider, A. Korber, S. Grabbe, and J. Dissemond Influence of pH on wound-healing: a new perspective for wound-therapy Arch. Dermatol. Res. 298 2007 413 420 (Pubitemid 46144202)
33. 2 J. Burn Care Res. 29 2008 49 55
34. T.B. Sparkman, T. Johns, T. Engerson, and H.P. Jones Activation- associated alterations in neutrophil pyridine nucleotide levels: a potential regulatory role for calcium and calmodulin Biochim. Biophys. Acta 846 1985 8 13 (Pubitemid 15012790)
35. I.M. Ahmad, B.E. Britigan, and M.Y. Abdalla Oxidation of thiols and modification of redox-sensitive signalling in human lung epithelial cells exposed to Pseudomonas pyocyanin J. Toxicol. Environ. Health A 74 2011 43 51
36. L. Ghibelli, S. Coppola, and G. Rotilio Lafavia; Maresca, V.; Ciriolo, M. R. Non-oxidative loss of glutathione in apoptosis via GSH extrusion Biochem. Biophys. Res. Commun. 216 1995 313 320
37. E.F. Nash, A. Stephenson, F. Ratjen, and E. Tullis Nebulized and oral thiol derivatives for pulmonary disease in cystic fibrosis Cochrane Database Syst. Rev. 1 2009 CD007168