A comparison of five treatment protocols for contaminated bone grafts in reference to sterility and cell viability

Journal of Bone and Joint Surgery

Volumn 93, Issue 5, 2011, Pages 439-444

  Bauer, Jennifer ^a   Liu, Raymond W ^a   Kean, Thomas J ^b   Dennis, James E ^b   Petersilge, William ^b   Gilmore, Allison ^a  

^a CASE WESTERN RESERVE UNIVERSITY   (United States)

^b CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHLORHEXIDINE GLUCONATE; POVIDONE IODINE; SODIUM CHLORIDE;

ADULT; AGED; ARTICLE; AUTOCLAVE; BACTERIAL GROWTH; BACTERIUM CONTAMINATION; BACTERIUM CULTURE; BONE GRAFT; CELL COUNT; CELL VIABILITY; CLINICAL ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; NONHUMAN; OSTEOARTHRITIS; PRIORITY JOURNAL; TOTAL KNEE REPLACEMENT;

EID: 79952412940     PISSN: 00219355     EISSN: 15351386     Source Type: Journal    
DOI: 10.2106/JBJS.J.00418     Document Type: Article

References (25)

1. Presnal BP, Kimbrough EE. What to do about a dropped bone graft. Clin Orthop Relat Res. 1993;296:310-1. (Pubitemid 23332156)
2. Suzuki A, Nambab Y, Matsuura M, Horisawa A. Bacterial contamination of floors and other surfaces in operating rooms: a five-year survey. J Hyg (London). 1984;93: 559-66. (Pubitemid 15181017)
3. Parker RD, Maschke SD. Mechanical agitation and serial dilution: an option for anterior cruciate ligament graft sterilization. J Knee Surg. 2008;21:186-91.
4. Dawson P, Han I, Cox M, Black C, Simmons L. Residence time and food contact time effects on transfer of Salmonella typhimurium from tile, wood, and carpet: testing the five-second rule. J Appl Microbiol. 2006;102:945-53. (Pubitemid 46457492)
5. Molina ME, Nonweiller DE, Evans JA, Delee JC. Contaminated anterior cruciate ligament grafts: the efficacy of 3 sterilization agents. Arthroscopy. 2000;16:373-8. (Pubitemid 30322495)
6. Hantes ME, Basdekis GK, Varitimidis SE, Giotikas D, Petinaki E, Malizos KN. Autograft contamination during preparation for anterior cruciate ligament reconstruction. J Bone Joint Surg Am. 2008;90:760-4. (Pubitemid 351499964)
7. Cruz NI, Cestero HJ, Cora ML. Management of contaminated bone grafts. Plast Reconstr Surg. 1981;68:411-4. (Pubitemid 11052686)
8. Soyer J, Rouil M, Castel O. The effect of 10% povidone-iodine solution on contaminated bone allografts. J Hosp Infect. 2002;50:183-7. (Pubitemid 37554872)
9. Stanford R, Solomon M, Levick M, Kohan L, Bell S. Sterilization of contaminated bone-tendon autografts using 10% povidone-iodine solution. Orthopaedics. 1999; 22:601-4. (Pubitemid 29283657)
10. GoebelME, Drez D Jr, Heck SB, Stoma MK. Contaminated rabbit patellar tendon grafts. In vivo analysis of disinfecting methods. Am J Sports Med. 1994;22:387-91.
11. Centeno RF, Desai AR, Watson ME. Management of contaminated autologous grafts in plastic surgery. Eplasty. 2008;8:e23.
12. Burd T, Conroy BP, Meyer SC, Allen WC. The effects of chlorhexidine irrigation solution on contaminated bone-tendon allografts. Am J Sports Med. 2000;28:241-4. (Pubitemid 30174195)
13. Gray JC, Elves MW. Osteogenesis in bone grafts after short-term storage and topical antibiotic treatment. An experimental study in rats. J Bone Joint Surg Br. 1981;63:441-5. (Pubitemid 11039529)
14. Schmidlin PR, Imfeld T, Sahrmann P, Tchouboukov A, Weber FE. Effect of short-time povidone-iodine application on osteoblast proliferation and differentiation. Open Dent J. 2009;3:208-12.
15. Cabral CT, Fernandes MH. In vitro comparison of chlorhexidine and povidoneiodine on the long-term proliferation and functional activity of human alveolar bone cells. Clin Oral Investig. 2007;11:155-64.
16. Köhler P, Glas JE, Larsson S, Kreicbergs A. Incorporation of nonviable bone grafts. Autoclaved autogeneic and frozen allogeneic bone grafts compared in the rabbit. Acta Orthop Scand. 1987;58:54-60.
17. Ricardo R, Phelan K. Counting and determining the viability of cultured cells. J Vis Exp. 2008;16:752.
18. Izquierdo R Jr, Cadet ER, Bauer R, Stanwood W, Levine WN, Ahmad CS. A survey of sports medicine specialists investigating the preferred management of contaminated anterior cruciate ligament grafts. Arthroscopy. 2005;21:1348-53. (Pubitemid 41735201)
19. Stoddart MJ, Furlong PI, Simpson A, Davies CM, Richards RG. A comparison of non-radioactive methods for assessing viability in ex vivo cultured cancellous bone: technical note. Eur Cell Mater. 2006;12:16-25. (Pubitemid 44898108)
20. Prentice AI. Autofluorescence of bone tissues. J Clin Pathol. 1967;20:717-9.
21. Gantenbein-Ritter B, Potier E, Zeiter S, van der Werf M, Sprecher CM, Ito K. Accuracy of three techniques to determine cell viability in 3D tissues or scaffolds. Tissue Eng Part C Methods. 2008;14:353-8.
22. Defino HL, da Silva Herrero CF, Crippa GE, Bellesini LS, Beloti MM, Rosa AL. In vitro proliferation and osteoblastic phenotype expression of cells derived from human vertebral lamina and iliac crest. Spine (Phila Pa 1976). 2009;34:1549-53.
23. Workman ML. Comparison of blot-drying versus air-drying of povidone-iodine-cleansed skin. Appl Nurs Res. 1995;8:15-17.
24. Kutarski PW, Grundy HC. To dry or not to dry? An assessment of the possible degradation in efficiency of preoperative skin preparation caused by wiping skin dry. Ann R Coll Surg Engl. 1993;75:181-5. (Pubitemid 23149844)
25. Digison MB. A review of anti-septic agents for preoperative skin preparation. Plast Surg Nurs. 2007;27:185-9.