Accuracy of three techniques to determine cell viability in 3D tissues or scaffolds

Tissue Engineering - Part C: Methods

Volumn 14, Issue 4, 2008, Pages 353-358

  Gantenbein Ritter, Benjamin ^a   Potier, Esther ^a,b   Zeiter, Stephan ^a   Van Der Werf, Marije ^a,b   Sprecher, Christoph M ^a   Ito, Keita ^b  

^a AO RESEARCH INSTITUTE   (Switzerland)

^b EINDHOVEN UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CELL CULTURE; CELL PROLIFERATION; CIVIL AVIATION; CORRELATION METHODS; CYTOLOGY; HISTOLOGY; POPULATION STATISTICS; RELIABILITY; SCAFFOLDS; SIZE DISTRIBUTION; THREE DIMENSIONAL;

ABSOLUTE ERRORS; ABSOLUTE QUANTIFICATIONS; BOVINE CHONDROCYTES; CALCEIN; CELL NUMBERS; CELL SHAPES; CELL VIABILITIES; CV VALUES; DEAD CELLS; HOMODIMER; INTRACLASS CORRELATIONS; LACTATE DEHYDROGENASES; RELIABILITY EVALUATIONS; SPATIAL DISTRIBUTION OF; TB METHODS;

TERBIUM ALLOYS;

CALCEIN; ETHIDIUM; LACTATE DEHYDROGENASE; TRYPAN BLUE;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARTILAGE CELL; CELL COUNT; CELL VIABILITY; CELLULAR DISTRIBUTION; CONTROLLED STUDY; COW; INTERMETHOD COMPARISON; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE; TISSUE ENGINEERING;

BOVINAE;

EID: 57349192376     PISSN: 19373384     EISSN: 19373392     Source Type: Journal    
DOI: 10.1089/ten.tec.2008.0313     Document Type: Article

References (19)

1. Stoddart, M.J., Furlong, P.I., Simpson, A., et al. A comparison of non-radioactive methods for assessing viability in ex vivo cultured cancellous bone: technical note. Eur Cell Mater 12, 16-25, 2006; discussion 16-25.
2. Rauch, B., Edwards, R.B., Lu, Y., et al. Comparison of techniques for determination of chondrocyte viability after thermal injury. Am J Vet Res 67, 1280, 2006.
3. Willerth, S.M., Arendas, K.J., Gottlieb, D.I., et al. Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells. Biomaterials 27, 5990, 2006.
4. Mauck, R.L., Yuan, X., and Tuan, R.S. Chondrogenic differentiation and functional maturation of bovine mesenchymal stem cells in long-term agarose culture. Osteoarthritis Cartilage 14, 179, 2006.
5. Park, S.H., Park, S.R., Chung, S.I., et al. Tissue-engineered cartilage using fibrin/hyaluronan composite gel and its in vivo implantation. Artif Organs 29, 838, 2005.
6. Catelas, I., Sese, N., Wu, B.M., et al. Human mesenchymal stem cell proliferation and osteogenic differentiation in fibrin gels in vitro. Tissue Eng 12, 1, 2006.
7. Ho, W., Tawil, B., Dunn, J.C., et al. The behavior of human mesenchymal stem cells in 3D fibrin clots: dependence on fibrinogen concentration and clot structure. Tissue Eng 12, 1587, 2006.
8. Haschtmann, D., Stoyanov, J.V., Ettinger, L., et al. Establishment of a novel intervertebral disc/endplate culture model: analysis of an ex vivo in vitro whole-organ rabbit culture system. Spine 31, 2918, 2006.
9. Melamed, M.R., Kamentsky, L.A., and Boyse, E.A. Cytotoxic test automation: a live-dead cell differential counter. Science 163, 285, 1969.
10. O'Brien, R., and Gottlieb-Rosenkrantz, P. An automatic method for viability assay of cultured cells. J Histochem Cytochem 18, 581, 1970.
11. Imbert, D., and Cullander, C. Assessment of cornea viability by confocal laser scanning microscopy and MTT assay. Cornea 16, 666, 1997.
12. Maroudas, A., Stockwell, R.A., Nachemson, A., et al. Factors involved in the nutrition of the human lumbar intervertebral disc: cellularity and diffusion of glucose in vitro. J Anat 120, 113, 1975.
13. Hunziker, E.B., Quinn, T.M., and Häuselmann, H.J. Quantitative structural organization of normal adult human articular cartilage. Osteoarthritis Cartilage 10, 564, 2002.
14. Rasband, W.S. ImageJ, National Institutes of Health, Bethesda, MD, http://rsb.info.nih.gov/ij/, 1997-2007, 2007.
15. Sokal, R.R., and Rohlf, F.J. Biometry. W.H. Freeman Company, New York, NY, 1995.
16. SPSS Inc. SPSS Base 14.0 for Windows User's Guide, 2007.
17. Shrout, P.E., and Fleiss, J.L. Intraclass correlations: uses in assessing rater reliability. Psychol Bull 84, 420, 1979.
18. Landis, J.R., and Koch, G.G. The measurement of observer agreement for categorical data. Biometrics 33, 159, 1977.
19. Lightfoot, A., Martin, J., and Amendola, A. Fluorescent viability stains overestimate chondrocyte viability in osteoarticular allografts. Am J Sports Med 35, 1817, 2007.