In vitro comparison of chlorhexidine and povidone-iodine on the long-term proliferation and functional activity of human alveolar bone cells

Clinical Oral Investigations

Volumn 11, Issue 2, 2007, Pages 155-164

  Cabral, Cristina Trigo ^a   Fernandes, Maria Helena ^a  

^a Portugal   (Portugal)

Author keywords

Chlorhexidine; Cytotoxicity profile; Dose effects; Human alveolar bone cells; Long term exposure; Povidone iodine

Indexed keywords

ALKALINE PHOSPHATASE; CALCIUM; CHLORHEXIDINE; PHOSPHATE; POVIDONE IODINE; TOPICAL ANTIINFECTIVE AGENT;

ADULT; ANALYSIS OF VARIANCE; ARTICLE; CELL ADHESION; CELL CULTURE; CELL PROLIFERATION; CELL SHAPE; CELL SURVIVAL; COMPARATIVE STUDY; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; HUMAN; JAW; METABOLISM; OSTEOBLAST;

ADULT; ALKALINE PHOSPHATASE; ALVEOLAR PROCESS; ANALYSIS OF VARIANCE; ANTI-INFECTIVE AGENTS, LOCAL; CALCIUM; CELL ADHESION; CELL PROLIFERATION; CELL SHAPE; CELL SURVIVAL; CELLS, CULTURED; CHLORHEXIDINE; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; OSTEOBLASTS; PHOSPHATES; POVIDONE-IODINE;

EID: 34249726648     PISSN: 14326981     EISSN: 14363771     Source Type: Journal    
DOI: 10.1007/s00784-006-0094-8     Document Type: Article

References (50)

1. Alexander RE (1998) Eleven miths of dentoalveolar surgery. J Am Dent Assoc 129:1271-1279
2. Amin MS, Harrison RL, Benton TS, Roberts M, Weinstein P (2004) Effect of povidone-iodine on Streptococcus mutans in children with extensive dental caries. Pediatr Dent 26:5-10
3. Amler MH (1969) The time sequence of tissue regeneration in human extraction wounds. Oral Surg 27:309
4. Babich H, Wurzburger BJ, Rubin YL, Sinensky MC, Blau L (1995) An in vitro study on the cytotoxicity of chlorhexidine gluconate to human gengival cells. Cell Biol Toxicol 11:79-88
5. Balin AK, Pratt L (2002) Dilute povidone-iodine solutions inhibit human skin fibroblast growth. Dermatol Surg 28:210-214
6. Bassetti C, Kallenberger A (1980) Influence of chlorhexidine rinsing on the healing of oral mucosa and osseous lesions. J Clin Periodontol 7:443-456
7. Bellows CG, Aubin JE, Heersche JNM (1991) Initiation and progression of mineralization of bone nodules formed in vitro: The role of alkaline phosphatase and organic phosphate. Bone Miner 14:27-40
8. Berridge MV, Tan AS (1993) Characterization of the cellular reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT): subcellular location, substracte dependence, and involvement of mitochondrial electron transport in MTT reduction. Arch Biochem Biophys 303:474-482
9. Berwick JE, Lessin ME (1990) Effects of a chlorhexidine gluconate oral rinse on the incidence of alveolar osteitis in mandibular third molar surgery. J Oral Maxillofac Surg 48:444-448
10. Bhandari M, Adili A, Schemitsch EH (2001) The efficacy of low pressure lavage with different irrigating solutions to remove adherent bacteria from bone. J Bone Jt Surg 83(A):412-419
11. Birn H (1973) Etiology and pathogenesis of fibrinolytic alveolitis (dry socket). Int J Oral Surg 2:211-263
12. Blum IR (2002) Comtemporary views on dry socket (alveolar osteitis): A clinical appraisal of standardization, aetiopathogenesis and management: a critical review. Int J Oral Maxillofac Surg 31:309-317
13. Bonine FL (1995) Effect of chlorhexidine rinse on the incidence of dry socket in impacted mandibular third molar extraction sites. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 79:154-158
14. Bouchlariotou I, Zahedi CS, von Ohle C, Brecx M (2001) Polyvidone iodée: Activité antimicrobiene en parodontologie. J Parad Implantol Oral 21:5-12
15. Bragger U, Schild U, Lang NP (1994) Effect of chlorhexidine (0.12%) rinses on periodontal tissue healing after tooth extraction. (II). Radiographic parameters. J Clin Periodontol 21:422-430
16. Branchet MCh, Boisnic S, Pascal F, Federlin-Ducani M (1994) Action in vitro d'une solution a base de chlorhexidine a 0,1% sur la prolifération des fibroblasts et la différenciation épithéliale de muqueuse reconstituée. J de Paradontologie 13:79-86
17. Chen YT, Hung SL, Lin LW, Chi LY, Ling LJ (2003) Attachment of periodontal ligament cells to chlorhexidine-loaded guided tissue regeneration membranes. J Periodontol 74:1652-1659
18. Cline NV, Layman DL (1992) The effects of chlorhexidine on the attachment and growth of cultured human periodontal cells. J Periodontol 63:598-602
19. Costa MA, Fernandes MH (2000) Long-term effects of parathyroid hormone, dihydroxyvitamin D3 and dexamethasone on the cell growth and functional activity of human osteogenic alveolar bone cell cultures. Pharmacol Res 42:345-353
20. Dajani AS, Taubert KA, Wilson W (1997) Prevention of bacterial endocarditis: Recommendation by the American Heart Association. J Am Dent Assoc 128:1142-1151
21. Damour O, Zhi HS, Lasne F, Villain M, Rousselle P, Collombel C (1992) Cytotoxicity evaluation of antiseptics and antibiotics on cultured human fibroblasts and keratinocytes. Burns 18:479-485
22. Delilbasi C, Saracoglu U, Keskin A (2002) Effects of 0.2% chlorhexidine gluconate and amoxicillin plus clavulanic acid on the prevention of alveolar osteitis following mandibular third molar extractions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 94:301-304
23. Elsen K, Ozmeric N, Sardas S (2002) Monitoring of buccal epithelial cells by alkaline comet assay (single cell gel electrophoresis technique) in cytogenetic evaluation of chlorhexidine. J Clin Oral Invest 6:150-154
24. Fernandes MH, Costa MA, Carvalho GS (1997) Mineralization in serially passaged human alveolar bone cells. J Mat Sci Mater Med 8:61-65
25. Gabler WL, Roberts D, Harold W (1987) The effects of chlorhexidine on blood cells. J Periodontal Res 22:150-155
26. Greenstein G (1999) Povidone-iodine's effects and role in the management of periodontal diseases: A review. J Periodontol 70:1397-1405
27. Gwynn IAP (1994) Cell biology at interfaces. J Mat Sci Mater Med 5:357-360
28. Hoang T, Jorgensen MG, Keim RG, Pattison AM, Slots J (2003) Povidone-iodine as a periodontal pocket disinfectant. J Periodontal Res 38:311-317
29. Kaysinger KK, Nicholson NC, Ramp WK, Kellam JF (1995) Toxic effects of wound irrigation solutions on cultured tibiae and osteoblasts. J Orthop Trauma 9:303-311
30. Lopez L, Berkowitz R, Spiekerman C, Weinstein P (2002) Topical antimicrobial therapy in the prevention of early childwood caries: A follow-up report. Pediatr Dent 24:204-206
31. Loukota RA (1991) The effect of pre-operative perioral skin preparation with aqueous povidone-iodine on the incidence of infection after third molar removal. Br J Oral Maxillofac Surg 29:336-337
32. MacGregor AJ, Hart P (1970) Bacteria of the extraction wound. J Oral Surg 28:885-887
33. Mariotti AJ, Rumpf D (1999) Chlorhexidine-induced changes to human gingival fibroblasts collagen and non-collagen protein production. J Periodontol 70:1443-1448
34. Nefussi J-R, Casamajor P, Serfaty R, Bolle M, Hugly C, Forest N (1998) Activated adult human alveolar bone cells: A new model of matrix mineralization. Eur J Oral Sci 106(Suppl 1):424-428
35. Osbom TP, Frederickson G, Small IA, Torgerson TS (1985) A prospective study of complications related to mandibular third molar surgery. J Oral Maxillofac Surg 43:767-769
36. Pucher JJ, Daniel JC (1992) The effects of chlorhexidine digluconate on human fibroblasts in vitro. J Periodontol 63:526-532
37. Rahn R, Schneiders, Diehl O, Schafer V, Shah P (1995) Preventing pot-treatment bacteriemia: Comparing topical povidone iodine and chlorhexidine. J Am Dental Ass 126:1145-1149
38. Ribeiro DA, Bazo AP, da Silva Franchi CA, Marques MEA, Salvadori DMF (2004) Chlorhexidine induces DNA damage in rat peripheral leukocytes and oral mucosal cells. J Periodontal Res 39:358-361
39. Rosling B, Hellstrom M-K, Ramberg P, Socransky SS, Lindhe J (2001) The use of PVP-iodine as an adjunct to non-surgical treatment of chronic periodontitis. J Clin Periodontol 28:1023-1031
40. Sanchez IR, Nusbaum KE, Swaim SF, Hale AS, Henderson RA, Mcguire JA (1988) Chlorhexidine diacetate and povidone-iodine cytotoxicity to canine embryonic fibroblasts and Staphylococcus aureus. Vet Surg 17:182-185
41. Segura JJ, Jiménez-Rubio A, Guerrero JM, Calvo JR (1999) Comparative effects of two endodontic irrigants, chlorhexidine digluconate and sodium hypochlorite on macrophage adhesion to plastic surfaces. J Endod 25:243-246
42. Seymour RA, Heasman PA, MacGregor IDM (1992) Anti-plaque and anti-calculus agents. In: Seymour RA, Heasman PA (eds) Drugs, diseases and the periodontium. Oxford Medical Publications, Hong Kong, pp 153-179
43. Shahan MH, Chuang AH, Brennan WA, Dirksen TR, van Dyke TE, Mcpherson JC (1993) The effect of chlorhexidine irrigation on tensile wound strength. J Periodontol 64:719-722
44. Slots J (2002) Selection of antimicrobial agents in periodontal therapy. J Periodontal Res 37:389-398
45. Stein GS, Lian JB, Stein JL, van Wijnen AJ, Frenkel B, Montecino M (1996) Mechanisms regulating osteoblast proliferation and differentiation. In: Bilizikian JP, Raisz LG, Rodan GA (eds) Principles of bone biology. Academic, New York, pp 69-86
46. Swanson AE (1990) Prevention of dry sockect: An overview. Oral Surg Oral Med Oral Pathol 70:131-136
47. Sweet JB, Macynski AA (1985) Effect of antimicrobial mouth rinses on the incidence of localized alveolitis and infection following mandibular third molar oral surgery. Oral Surg Oral Med Oral Pathol 59:24-26
48. Tatnal FM, Leighim, Gibson JR (1990) Comparative study of antiseptic toxicity on basal keratinocytes, tranformed human keratinocytes and fibroblasts. Skin Pharmacol 3:157-163
49. Teepe RG, Koebrugge EJ, Lowik CW, Petit PL, Bosboom RW, Twiss IM, Boxma H, Vermeer BJ, Ponec M (1993) Cytotoxic effects of topical antimicrobial and antiseptic agents on human keratinocytes in vitro. J Trauma 35:8-19
50. Wilken R, Botha SJ, Grober A, Germishuys PJ (2001) In vitro cytotoxicity of chlorhexidine gluconate, benzydamine-HCL and povidone iodine mouthrinses on human gingival fibroblasts. SADJ 56:455-460