Detection of Klebsiella pneumoniae Carbapenemase (KPC) production in non-Klebsiella pneumoniae Enterobacteriaceae isolates by use of the Phoenix, Vitek 2, and disk diffusion methods

Journal of Clinical Microbiology

Volumn 49, Issue 3, 2011, Pages 1143-1147

  Doern, Christopher D ^a   Michael Dunne Jr , W ^b   Burnham, Carey Ann D ^b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBAPENEMASE; DORIPENEM; ERTAPENEM; IMIPENEM; MEROPENEM;

ACCURACY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIUM DETECTION; BACTERIUM ISOLATE; CITROBACTER FREUNDII; CONTROLLED STUDY; DISK DIFFUSION; ENTEROBACTER AEROGENES; ENTEROBACTER CLOACAE; ENTEROBACTERIACEAE; ESCHERICHIA COLI; KLEBSIELLA OXYTOCA; KLEBSIELLA PNEUMONIAE; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; PROTEUS MIRABILIS; SENSITIVITY AND SPECIFICITY;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; BETA-LACTAMASES; BETA-LACTAMS; ENTEROBACTERIACEAE; HUMANS; MICROBIAL SENSITIVITY TESTS;

ENTEROBACTERIACEAE; KLEBSIELLA PNEUMONIAE;

EID: 79952327500     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02163-10     Document Type: Article

References (19)

1. Bratu, S., D. Landman, M. Alam, E. Tolentino, and J. Quale. 2005. Detection of KPC carbapenem-hydrolyzing enzymes in Enterobacter spp. from Brooklyn, New York. Antimicrob. Agents Chemother. 49:776-778.
2. Bulik, C. C., et al. 2010. Comparison of meropenem MICs and susceptibilities for carbapenemase-producing Klebsiella pneumoniae isolates by various testing methods. J. Clin. Microbiol. 48:2402-2406.
3. Carvalhaes, C. G., R. C. Picao, A. G. Nicoletti, D. E. Xavier, and A. C. Gales. 2010. Cloverleaf test (modified Hodge test) for detecting carbapenemase production in Klebsiella pneumoniae: be aware of false positive results. J. Antimicrob. Chemother. 65:249-251.
4. Clinical and Laboratory Standards Institute. 2009. Performance standards for antimicrobial susceptibility testing. Nineteenth informational supplement. Document M100-S19. CLSI, Wayne, PA.
5. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement. Document M100-S20. CLSI, Wayne, PA.
6. Clinical and Laboratory Standards Institute. 2010. Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement update. Document M100-S20 U. CLSI, Wayne, PA.
7. D'Alincourt Carvalho-Assef, A. P., et al. 2010. Escherichia coli producing KPC-2 carbapenemase: first report in Brazil. Diagn. Microbiol. Infect. Dis. 68:337-338.
8. Fisher, M. A., et al. 2009. Performance of the Phoenix bacterial identification system compared with disc diffusion methods for identifying extended-spectrum beta-lactamase, AmpC and KPC producers. J. Med. Microbiol. 58:774-778.
9. Livermore, D. M., R. Hope, G. Brick, M. Lillie, and R. Reynolds. 2008. Non-susceptibility trends among Enterobacteriaceae from bacteraemias in the UK and Ireland, 2001-06. J. Antimicrob. Chemother. 62(Suppl. 2):ii41-ii54.
10. Marchaim, D., S. Navon-Venezia, M. J. Schwaber, and Y. Carmeli. 2008. Isolation of imipenem-resistant Enterobacter species: emergence of KPC-2 carbapenemase, molecular characterization, epidemiology, and outcomes. Antimicrob. Agents Chemother. 52:1413-1418.
11. Pournaras, S., et al. 2010. Detection of the new metallo-beta-lactamase VIM-19 along with KPC-2, CMY-2 and CTX-M-15 in Klebsiella pneumoniae. J. Antimicrob. Chemother. 65:1604-1607.
12. Tibbetts, R., J. G. Frye, J. Marschall, D. Warren, and W. Dunne. 2008. Detection of KPC-2 in a clinical isolate of Proteus mirabilis and first reported description of carbapenemase resistance caused by a KPC betalactamase in P. mirabilis. J. Clin. Microbiol. 46:3080-3083.
13. Tsakris, A., et al. 2009. Evaluation of boronic acid disk tests for differentiating KPC-possessing Klebsiella pneumoniae isolates in the clinical laboratory. J. Clin. Microbiol. 47:362-367.
14. Tsakris, A., et al. 2009. Use of boronic acid disk tests to detect extended-spectrum beta-lactamases in clinical isolates of KPC carbapenemase-possessing enterobacteriaceae. J. Clin. Microbiol. 47:3420-3426.
15. Vading, M., O. Samuelsen, B. Haldorsen, A. S. Sundsfjord, and C. G. Giske. 23 July 2010. Comparison of disk diffusion, Etest and VITEK2 for detection of carbapenemase-producing Klebsiella pneumoniae with EUCAST and CLSI breakpoint systems. Clin. Microbiol. Infect. [Epub ahead of print.] doi:10.1111/j.1469-0691.2010.03299.x.
16. Weisenberg, S. A., D. J. Morgan, R. Espinal-Witter, and D. H. Larone. 2009. Clinical outcomes of patients with Klebsiella pneumoniae carbapenemase-producing K. pneumoniae after treatment with imipenem or meropenem. Diagn. Microbiol. Infect. Dis. 64:233-235.
17. Woodford, N., et al. 2010. Comparison of BD Phoenix, Vitek 2, and MicroScan automated systems for detection and inference of mechanisms responsible for carbapenem resistance in Enterobacteriaceae. J. Clin. Microbiol. 48:2999-3002.
18. Yigit, H., et al. 2001. Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 45:1151-1161.
19. Zarkotou, O., et al. 2010. Risk factors and outcomes associated with acquisition of colistin-resistant KPC-producing Klebsiella pneumoniae: a matched case-control study. J. Clin. Microbiol. 48:2271-2274.