Isolation and characterization of a hydrogen- and ethanol-producing Clostridium sp. strain URNW

Canadian Journal of Microbiology

Volumn 57, Issue 3, 2011, Pages 236-243

  Ramachandran, Umesh ^a   Wrana, Nathan ^a   Cicek, Nazim ^a   Sparling, Richard ^a   Levin, David B ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Biofuels; Cellobiose; Clostridium sp; Ethanol; Hydrogen; Phylogenetics

Indexed keywords

16S RDNA; A-CARBON; BATCH CULTURE; BLAST ANALYSIS; CELL GENERATION; CELLOBIOSE; CHAPERONIN; DRY CELLS; END-PRODUCTS; ISOLATION AND CHARACTERIZATION; MESOPHILIC; METABOLIC PROFILING; NUCLEOTIDE SEQUENCES; PHYLOGENETIC ANALYSIS; PHYLOGENETICS; PYRUVATES; SPECIFIC PRODUCTION RATE; SUGAR ALCOHOLS;

BATCH CELL CULTURE; CARBON DIOXIDE; CELL CULTURE; ETHANOL; GLUCOSE; HYDROGEN; SUGAR (SUCROSE); VOLATILE FATTY ACIDS;

CLOSTRIDIUM;

ACETIC ACID; ALCOHOL; BUTYRIC ACID; CARBON DIOXIDE; CELLOBIOSE; CHAPERONIN; DNA 16S; FORMIC ACID; GLUCOSE; HYDROGEN; LACTIC ACID; MANNITOL; MANNOSE; PYRUVIC ACID; SORBITOL; SUCROSE;

BACTERIUM; BIOFUEL; CARBON DIOXIDE; ETHANOL; GLUCOSE; HYDROGEN; IDENTIFICATION METHOD; METABOLISM; PHYLOGENETICS;

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIAL STRAIN; BACTERIUM ISOLATION; CLOSTRIDIUM; DNA SEQUENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

CARBON DIOXIDE; CELLOBIOSE; CLOSTRIDIUM; CULTURE MEDIA; DNA, BACTERIAL; DNA, RIBOSOMAL; ETHANOL; HYDROGEN; HYDROGEN-ION CONCENTRATION; PHYLOGENY; RNA, RIBOSOMAL, 16S; SEQUENCE ANALYSIS, DNA;

CLOSTRIDIA; CLOSTRIDIUM; CLOSTRIDIUM INTESTINALE; CLOSTRIDIUM SP.;

EID: 79952209184     PISSN: 00084166     EISSN: 14803275     Source Type: Journal    
DOI: 10.1139/W11-005     Document Type: Article

References (25)

1. Berndes, G., Hoogwijk, M., and van den Broek, R. 2003. The con-tribution of biomass in the future global energy supply: a review of 17 studies. Biomass Bioenergy, 25(1): 1-28. doi:10.1016/ S0961-9534(02)00185-X.
2. Chen, W.-M., Tseng, Z.-J., Lee, K.-S., and Chang, J.-S. 2005. Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge. Int. J. Hydrogen Energy, 30(10): 1063-1070. doi:10.1016/j.ijhydene.2004.09.008.
3. Daniels, L., Belay, N., and Rajagopal, B.S. 1986. Assimilatory reduction of sulfate and sulfite by methanogenic bacteria. Appl. Environ. Microbiol. 51(4): 703-709. PMID:3707121.
4. Darrett, R. 1995. Biochemistry. Saunders College Publishing, New York.
5. Desvaux, M., Guedon, E., and Petitdemange, H. 2000. Cellulose catabolism by Clostridium cellulolyticum growing in batch culture on defined medium. Appl. Environ. Microbiol. 66(6): 2461-2470. doi:10.1128/AEM.66.6.2461-2470.2000. PMID: 10831425.
6. Dumonceaux, T.J., Hill, J.E., Briggs, S.A., Amoako, K.K., Hemmingsen, S.M., and Van Kessel, A.G. 2006. Enumeration of specific bacterial populations in complex intestinal communities using quantitative PCR based on the chaperonin-60 target. J. Microbiol. Methods, 64(1): 46-62. doi:10.1016/j.mimet.2005.04.006. PMID:16112762.
7. Giallo, J., Gaudin, C., Belaich, J.P., Petitdemange, E., and Caillet-Mangin, F. 1983. Metabolism of glucose and cellobiose by a cellulolytic mesophilic Clostridiuim sp. strain H1O. Appl. Environ. Microbiol. 45(3): 843-849. PMID:6847188.
8. Goh, S.H., Santucci, Z., Kloos, W.E., Faltyn, M., George, C.G., Driedger, D., and Hemmingsen, S.M. 1997. Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization. J. Clin. Microbiol. 35(12): 3116-3121. PMID:9399505.
9. Goh, S.H., Facklam, R.R., Chang, M., Hill, J.E., Tyrrell, G.J., Burns, E.C.M., et al. 2000. Identification of Enterococcus species and phenotypically similar Lactococcus and Vagococcus species by reverse checkerboard hybridization to chaperonin 60 gene sequences. J. Clin. Microbiol. 38(11): 3953-3959. PMID: 11060051.
10. Gössner, A.S., Küsel, K., Schulz, D., Trenz, S., Acker, G., Lovell, C.R., and Drake, H.L. 2006. Trophic interaction of the aerotolerant anaerobe Clostridium intestinale and the acetogen Sporomusa rhizae sp. nov. isolated from roots of the black needlerush Juncus roemerianus. Microbiology, 152(4): 1209-1219. doi:10. 1099/mic.0.28725-0. PMID:16549683.
11. Guedon, E., Payot, S., Desvaux, M., and Petitdemange, H. 1999. Carbon and electron flow in Clostridium cellulolyticum grown in chemostat culture on synthetic medium. J. Bacteriol. 181(10): 3262-3269. PMID:10322031.
12. Hill, J.E., Penny, S.L., Crowell, K.G., Goh, S.H., and Hemmingsen, S.M. 2004. cpnDB: a chaperonin sequence database. Genome Res. 14(8): 1669-1675. doi:10.1101/gr.2649204. PMID:15289485.
13. Hoogwijk, M., Faaija, A., van den Broek, R., Berndes, G., Gielen, D., and Turkenburg, W. 2003. Exploration of the ranges of the global potential of biomass for energy. Biomass Bioenergy, 25(2): 119-133.
14. Jian, W., Zhu, L., and Dong, X.Z. 2001. New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. Int. J. Syst. Evol. Microbiol. 51(Pt 5): 1633-1638. PMID:11594590.
15. Lee, W.-K., Fujisawa, T., Kawamura, S., Itoh, K., and Mitsuoka, T. 1989. Clostridium intestinalis sp. nov., and aerotolerant species isolated from the feces of cattle and pigs. Int. J. Syst. Bacteriol. 39(3): 334-336. doi:10.1099/00207713-39-3-334.
16. Levin, D.B., Zhu, H., Beland, M., Cicek, N., and Holbein, B.E. 2007. Potential for hydrogen and methane production from biomass residues in Canada. Bioresour. Technol. 98(3): 654-660. doi:10.1016/j.biortech.2006.02.027. PMID:16580198.
17. Mabee, W.E., Gregg, D.J., and Saddler, J.N. 2005. Assessing the emerging biorefinery sector in Canada. Appl. Biochem. Biotechnol. 123(1-3): 765-778. doi:10.1385/ABAB:123:1-3:0765. PMID: 15930556.
18. Peguin, S., and Soucaille, P. 1995. Modulation of carbon and electron flow in Clostridium acetobutylicum by iron limitation and methyl viologen addition. Appl. Environ. Microbiol. 61(1): 403-405. PMID:16534918.
19. Ramachandran, U., Wrana, N., Cicek, N., Sparling, R., and Levin, D.B. 2008. Hydrogen production and end-product synthesis patterns by Clostridium termitidis strain CT1112 in batch fermentation cultures with cellobiose or α-cellulose. Int. J. Hydrogen Energy, 33(23): 7006-7012. doi:10.1016/j.ijhydene.2008.09.022.
20. Rydzak, T., Levin, D.B., Cicek, N., and Sparling, R. 2009. Growth phase dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405. J. Biotechnol. 140(3-4): 169-175. doi:10.1016/j.jbiotec. 2009.01.022. PMID:19428711.
21. Saitou, N., and Nei, M. 1987. The neighbor-joining method -a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4(4): 406-425. PMID:3447015.
22. Sander, R. 1999. Compilation of Henry's law constants for inorganic and organic species of potential importance in environmental chemistry [online]. Available from http://www. mpch-mainz.mpg.de/~sander/res/henry.html [accessed March 2010].
23. Tamura, K., Nei, M., and Kumar, S. 2004. Prospects for inferring very large phylogenies by using the neighbor-joining method. Proc. Natl. Acad. Sci. U.S.A. 101(30): 11 030 -11 035. doi:10. 1073/pnas.0404206101. PMID:15258291.
24. Tamura, K., Dudley, J., Nei, M., and Kumar, S. 2007. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol. Biol. Evol. 24(8): 1596-1599. doi:10.1093/ molbev/msm092. PMID:17488738.
25. Zhang, H., Bruns, M.A., and Logan, B.E. 2006. Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. Water Res. 40(4): 728-734. doi:10.1016/j.watres. 2005.11.041. PMID:16427113.