Studies of Proteoglycan Involvement in CPP-Mediated Delivery

Methods in Molecular Biology

Volumn 683, Issue , 2011, Pages 99-115

  Wittrup, Anders ^a   Zhang, Si He ^a   Belting, Mattias ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

Cell penetrating peptides; CHO cell mutants; Glypican; Heparan sulfate; Proteoglycan; siRNA; Syndecan

Indexed keywords

2 DIETHYLAMINOETHANOL; 2-DIETHYLAMINOETHANOL; CELL PENETRATING PEPTIDE; ETHANOLAMINE DERIVATIVE; PROTEOGLYCAN; SYNDECAN 2;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; CHO CELL; CRICETULUS; GENE SILENCING; GENETICS; HAMSTER; HELA CELL; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; PROTEIN TRANSPORT;

ANIMALS; CELL-PENETRATING PEPTIDES; CHO CELLS; CRICETINAE; CRICETULUS; ETHANOLAMINES; GENE KNOCKDOWN TECHNIQUES; HELA CELLS; HUMANS; PROTEIN TRANSPORT; PROTEOGLYCANS; SYNDECAN-2;

MLCS; MLOWN;

EID: 79952202646     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-919-2_8     Document Type: Chapter

References (25)

1. Poon, G. M., and Gariepy, J. (2007) Cell-surface proteoglycans as molecular portals for cationic peptide and polymer entry into cells, Biochem Soc Trans 35, 788–793.
2. Mislick, K. A., and Baldeschwieler, J. D. (1996) Evidence for the role of proteoglycans in cation-mediated gene transfer, Proc Natl Acad Sci U S A 93, 12349–12354.
3. Console, S., Marty, C., Garcia-Echeverria, C., Schwendener, R., and Ballmer-Hofer, K. (2003) Antennapedia and HIV transactivator of transcription (TAT) “protein transduction domains” promote endocytosis of high molecular weight cargo upon binding to cell surface glycosaminoglycans, J Biol Chem 278, 35109–35114.
4. Tyagi, M., Rusnati, M., Presta, M., and Giacca, M. (2001) Internalization of HIV-1 tat requires cell surface heparan sulfate proteoglycans, J Biol Chem 276, 3254–3261.
5. Sandgren, S., Cheng, F., and Belting, M. (2002) Nuclear targeting of macromolecular polyanions by an HIV-Tat derived peptide. Role for cell-surface proteoglycans, J Biol Chem 277, 38877–38883.
6. Sandgren, S., Wittrup, A., Cheng, F., Jonsson, M., Eklund, E., Busch, S., and Belting, M. (2004) The human antimicrobial peptide LL-37 transfers extracellular DNA plasmid to the nuclear compartment of mammalian cells via lipid rafts and proteoglycan-dependent endocytosis, J Biol Chem 279, 17951–17956.
7. Tkachenko, E., Lutgens, E., Stan, R. V., and Simons, M. (2004) Fibroblast growth factor 2 endocytosis in endothelial cells proceed via syndecan-4-dependent activation of Rac1 and a Cdc42-dependent macropinocytic pathway, J Cell Sci 117, 3189–3199.
8. Yanagishita, M., and Hascall, V. C. (1984) Metabolism of proteoglycans in rat ovarian granulosa cell culture. Multiple intracellular degradative pathways and the effect of chloroquine, J Biol Chem 259, 10270–10283.
9. Argyris, E. G., Kulkosky, J., Meyer, M. E., Xu, Y., Mukhtar, M., Pomerantz, R. J., and Williams, K. J. (2004) The perlecan heparan sulfate proteoglycan mediates cellular uptake of HIV-1 Tat through a pathway responsible for biological activity, Virology 330, 481–486.
10. Nakase, I., Tadokoro, A., Kawabata, N., Takeuchi, T., Katoh, H., Hiramoto, K., Negishi, M., Nomizu, M., Sugiura, Y., and Futaki, S. (2007) Interaction of arginine-rich peptides with membrane-associated proteoglycans is crucial for induction of actin organization and macropinocytosis, Biochemistry 46, 492–501.
11. Wittrup, A., Zhang, S. H., Ten Dam, G. B., van Kuppevelt, T. H., Bengtson, P., Johansson, M., Welch, J., Morgelin, M., and Belting, M. (2009) ScFv antibody-induced translocation of cell-surface heparan sulfate proteoglycan to endocytic vesicles: Evidence for heparan sulfate epitope specificity and role of both syndecan and glypican, J Biol Chem 284, 32959–32967.
12. Landgraf, P., Wahle, P., Pape, H. C., Gundelfinger, E. D., and Kreutz, M. R. (2008) The survival-promoting peptide Y-P30 enhances binding of pleiotrophin to synde-can-2 and-3 and supports its neuritogenic activity, J Biol Chem 283, 25036–25045.
13. Zhang, L., Lawrence, R., Frazier, B. A., and Esko, J. D. (2006) CHO glycosylation mutants: proteoglycans, Methods Enzymol 416, 205–221.
14. Welch, J. E., Bengtson, P., Svensson, K., Wittrup, A., Jenniskens, G. J., Ten Dam, G. B., Van Kuppevelt, T. H., and Belting, M. (2008) Single chain fragment anti-heparan sulfate antibody targets the polyamine transport system and attenuates polyamine-dependent cell proliferation, Int J Oncol 32, 749–756.
15. Dennissen, M. A., Jenniskens, G. J., Pieffers, M., Versteeg, E. M., Petitou, M., Veerkamp, J. H., and van Kuppevelt, T. H. (2002) Large, tissue-regulated domain diversity of heparan sulfates demonstrated by phage display antibodies, J Biol Chem 277, 10982–10986.
16. David, G., Bai, X. M., Van der Schueren, B., Cassiman, J. J., and Van den Berghe, H. (1992) Developmental changes in heparan sulfate expression: in situ detection with mAbs, J Cell Biol 119, 961–975.
17. Belting, M., and Petersson, P. (1999) Intracellular accumulation of secreted proteoglycans inhibits cationic lipid-mediated gene transfer. Co-transfer of glycosaminoglycans to the nucleus, J Biol Chem 274, 19375–19382.
18. Belting, M., and Petersson, P. (1999) Protective role for proteoglycans against cationic lipid cytotoxicity allowing optimal transfection efficiency in vitro, Biochem J 342(Pt 2), 281–286.
19. Wittrup, A., and Belting, M. (2009) Characterizing peptide-mediated DNA internalization in human cancer cells, Methods Mol Biol 480, 101–112.
20. Iozzo, R. V. (2001) Proteoglycan Protocols, Humana Press, Totowa, New Jersey.
21. Humphries, D. E., and Silbert, J. E. (1988) Chlorate: a reversible inhibitor of proteoglycan sulfation, Biochem Biophys Res Commun 154, 365–371.
22. Inatani, M., Irie, F., Plump, A. S., Tessier-Lavigne, M., and Yamaguchi, Y. (2003) Mammalian brain morphogenesis and midline axon guidance require heparan sulfate, Science 302, 1044–1046.
23. Bullock, S. L., Fletcher, J. M., Beddington, R. S., and Wilson, V. A. (1998) Renal agenesis in mice homozygous for a gene trap mutation in the gene encoding heparan sulfate 2-sulfo-transferase, Genes Dev 12, 1894–1906.
24. Wittrup, A., Sandgren, S., Lilja, J., Bratt, C., Gustavsson, N., Morgelin, M., and Belting, M. (2007) Identification of proteins released by mammalian cells that mediate DNA internalization through proteoglycan-dependent macropinocytosis, J Biol Chem 282, 27897–27904.
25. van den Born, J., Salmivirta, K., Henttinen, T., Ostman, N., Ishimaru, T., Miyaura, S., Yoshida, K., and Salmivirta, M. (2005) Novel heparan sulfate structures revealed by monoclonal antibodies, J Biol Chem 280, 20516–20523.