Fluorescence imaging of membrane dynamics in living cells

Journal of Biomedical Optics

Volumn 15, Issue 4, 2010, Pages

  Weber, Petra ^a   Wagner, Michael ^a   Schneckenburger, Herbert ^a  

^a AALEN UNIVERSITY   (Germany)

Author keywords

Cholesterol; Fluorescence anisotropy; Fluorescence microscopy; Laurdan; Living cells; Membranes; Total internal reflection

Indexed keywords

CELL AGING; DRUG RESISTANCE; FLUORESCENCE ANISOTROPY; FLUORESCENCE IMAGING; FLUORESCENCE SPECTRA; INTRACELLULAR MEMBRANES; LAURDAN; LIVING CELL; MEMBRANE DYNAMICS; MEMBRANE FLUIDITY; RED-SHIFTED; ROTATIONAL CORRELATION TIME; SPECTRAL IMAGING; TOTAL INTERNAL REFLECTIONS; WIDE-FIELD;

ANISOTROPY; CELL MEMBRANES; CHOLESTEROL; ELECTROMAGNETIC FIELDS; FLUORESCENCE; FLUORESCENCE MICROSCOPY; NAPHTHALENE; REFRACTIVE INDEX; STIFFNESS;

MEMBRANES;

ANIMAL; ANISOTROPY; ARTICLE; CELL MEMBRANE; COMPUTER ASSISTED DIAGNOSIS; FLUORESCENCE MICROSCOPY; HUMAN; MEMBRANE FLUIDITY; METHODOLOGY; PHYSIOLOGY; ULTRASTRUCTURE; YOUNG MODULUS;

ANIMALS; ANISOTROPY; CELL MEMBRANE; ELASTIC MODULUS; HUMANS; IMAGE INTERPRETATION, COMPUTER-ASSISTED; MEMBRANE FLUIDITY; MICROSCOPY, FLUORESCENCE;

EID: 79952198713     PISSN: 10833668     EISSN: 15602281     Source Type: Journal    
DOI: 10.1117/1.3470446     Document Type: Article

References (26)

1. M. Sok, M. Senturc, M. Schara, J. Stare, and T. Rott, "Cell membrane fluidity and prognosis of lung cancer," Ann. Thorac. Surg. 73, 1567-1571 (2002). (Pubitemid 34596594)
2. N. B. Chauhan, "Membrane dynamics, cholesterol homeostasis, and Alzheimer's disease," J. Lipid Res. 44(11), 2019-2029 (2003). (Pubitemid 37509617)
3. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, Plenum Press, New York and London (2006).
4. N. L. Thompson, A.-W. Drake, L. Chen, and W. V. Broek, "Equilibrium, kinetics, diffusion and self-association of proteins at membrane surfaces: measurement by total internal reflection fluorescence microscopy," Photochem. Photobiol. 65, 39-46 (1997). (Pubitemid 27084359)
5. T. Parasassi, G. de Stasio, A. d'Ubaldo, and E. Gratton, "Phase fluctuation in phospholipid membranes revealed by laurdan fluorescence," Biophys. J. 57, 1179-1186 (1990). (Pubitemid 20203441)
6. T. Parasassi, E. K. Krasnowska, L. Bagatolli, and E. Gratton, "Laurdan and prodan as polarity-sensitive fluorescent membrane probes," J. Fluoresc. 6, 365-373 (1998). (Pubitemid 128545086)
7. T. Parasassi, G. de Stasio, G. Ravagnan, R. M. Rusch, and E. Gratton, "Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of laurdan fluorescence," Biophys. J. 60, 179-189 (1991).
8. D. Axelrod, "Cell-substrate contacts illuminated by total internal reflection fluorescence," J. Cell Biol. 89, 141-145 (1981). (Pubitemid 11066139)
9. H. Schneckenburger, "Total internal reflection fluorescence microscopy: technical innovations and novel applications," Curr. Opin. Biotechnol. 16, 13-18 (2005). (Pubitemid 40249754)
10. K. Simons and R. Ehehalt, "Cholesterol, lipid rafts, and disease," J. Clin. Invest. 110, 597-603 (2002). (Pubitemid 34988781)
11. G. P. Eckert, N. J. Cairns, A. Maras, W. F. Gattaz, and W. E. Muller, "Cholesterol modulates the membrane-disordering effects of betaamloyid peptides in the hippocampus: specific changes in Alzheimer's disease," Dementia Geriatr. Cognit. Disord. 11, 181-186 (2000). (Pubitemid 30416021)
12. C. A. F. von Arnim, B. von Einem, P. Weber, M. Wagner, D. Schwanzar, R. Spoelgen, W. S. L. Strauss, and H. Schneckenburger, "Impact of cholesterol level upon APP and BACE proximity and APP cleavage," Biochem. Biophys. Res. Commun. 370, 207-212 (2008).
13. C. H. Storch, R. Eehehalt, E. Haefeli, and J. Weiss, "Localization of the human breast cancer resistance protein (BCRP/ABCG2) in lipid rafts/caveolae and modulation of its activity in vitro," J. Pharmacol. Exp. Ther. 323, 257-264 (2007). (Pubitemid 47443266)
14. T. Parasassi, M. di Stefano, G. Ravagnan, O. Sapora, and E. Gratton, "Membrane aging during cell growth ascertained by laurdan generalized polarization," Exp. Cell Res. 202, 432-439 (1992).
15. A. E. Christian, M. P. Haynes, M. C. Phillips, and G. H. Rothblat, "Use of cyclodextrins for manipulating cellular cholesterol content," J. Lipid Res. 38, 2264-2272 (1997). (Pubitemid 27495385)
16. K. Stock, R. Sailer, W. S. L. Strauss, M. Lyttek, R. Steiner, and H. Schneckenburger, "Variable-angle total internal reflection fluorescence microscopy (VA-TIRFM): realization and application of a compact illumination device," J. Microsc. 211, 19-29 (2003). (Pubitemid 41709848)
17. C. G. Coates, D. J. Denvir, N. G. McHale, K. D. Thornbury, and M. A. Hollywood, "Optimizing low-light microscopy with backilluminated electron multiplying charge-coupled device: enhanced sensitivity, speed, and resolution," J. Biomed. Opt. 9(6), 1244-1252 (2004). (Pubitemid 40168140)
18. H. Schneckenburger, M. H. Gschwend, R. Sailer, H.-P. Mock, and W. S. L. Strauss, "Time-gated fluorescence microscopy in molecular and cellular biology," Cell. Mol. Biol. (Paris) 44, 795-805 (1998).
19. H. Schneckenburger, M. Wagner, M. Kretzschmar, W. S. L. Strauss, and R. Sailer, "Laser-assisted fluorescence microscopy for measuring cell membrane dynamics," Photochem. Photobiol. Sci. 3, 817-822 (2004). (Pubitemid 39302984)
20. P. Weber, M. Wagner, W. S. L. Strauss, and H. Schneckenburger, "Fluorescence imaging of cholesterol and temperature dependent cell membrane dynamics," in Biophotonics 2007: Optics in Life Science, J. Popp and G. V. Bally, Eds., Proc. SPIE 6633, 663305 (2007).
21. C. F. Chapman, Y. Liu, G. J. Sonek, and B. Tromberg, "The use of exogenous fluorescent probes for temperature measurements in single living cells," Photochem. Photobiol. 62, 416-425 (1995).
22. F. M. Harris, K. B. Best, and J. D. Bell, "Use of laurdan fluorescence intensity and polarization to distinguish between changes in membrane fluidity and phospholipid order," Biochim. Biophys. Acta 1565, 123-128 (2002). (Pubitemid 35239840)
23. W. Yu, P. T. C. So, T. French, and E. Gratton, "Fluorescence general polarization of cell membranes: a two-photon scanning microscopy approach," Biophys. J. 70, 626-636 (1996). (Pubitemid 26028279)
24. K. Gaus, S. Le Lay, N. Balasubramanian, and M. A. Schwartz, "Integrin mediated adhesion regulates membrane order," J. Cell Biol. 174, 725-734 (2006). (Pubitemid 44306728)
25. K. Gaus, T. Zech, and T. Harder, "Visualizing membrane microdomains by laurdan 2-photon microscopy," Mol. Membr Biol. 23, 41-48 (2006).
26. M. Wagner, P. Weber, W. S. L. Strauss, H.-P. Lassalle, and H. Schneckenburger, "Nanotomography of cell surfaces with evanescent fields," Adv. Opt. Technol., article ID 254317 (2008).