Site-directed (IS30-FljA) transposon mutagenesis system to produce nonflagellated mutants of SalmonellaEnteritidis

FEMS Microbiology Letters

Volumn 317, Issue 1, 2011, Pages 52-59

  Imre, Ariel ^a   Olasz, Ferenc ^b   Nagy, Béla ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Chimeric protein; Flagellin; fli operon; IS30; Salmonella; Site directed mutagenesis

Indexed keywords

FLAGELLIN; PROTEIN FLIC; PROTEIN FLID; PROTEIN FLJA; PROTEIN FLJB; TRANSPOSASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL STRAIN; BACTERIUM MUTANT; CONTROLLED STUDY; NONHUMAN; OPERON; PLASMID; PRIORITY JOURNAL; PROTEIN DNA BINDING; SALMONELLA ENTERITIDIS; SITE DIRECTED MUTAGENESIS; TRANSPOSON;

BACTERIAL PROTEINS; DNA TRANSPOSABLE ELEMENTS; FLAGELLA; GENE TARGETING; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; PLASMIDS; REPRESSOR PROTEINS; SALMONELLA ENTERITIDIS;

SALMONELLA; SALMONELLA ENTERITIDIS;

EID: 79952173878     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2011.02210.x     Document Type: Article

References (26)

1. Adriaensen C, De Greve H, Tian JQ, De Craeye S, Gubbels E, Eeckhaut V, Van Immerseel F, Ducatelle R, Kumar M & Hernalsteens JP (2007) A live Salmonella enterica serovar Enteritidis vaccine allows serological differentiation between vaccinated and infected animals. Infect Immun 75: 2461-2468.
2. Altschul SF, Gish W, Miller W, Myers ED & Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
3. Bushman FD (1994) Tethering human immunodeficiency virus 1 integrase to a DNA site directs integration to nearby sequences. P Natl Acad Sci USA 91: 9233-9237.
4. Coates CJ, Kaminski JM, Summers JB, Segal DJ, Miller AD & Kolb AF (2005) Site-directed genome modification: derivatives of DNA-modifying enzymes as targeting tools. Trends Biotechnol 23: 407-419.
5. Datsenko KA & Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. P Natl Acad Sci USA 97: 6640-6645.
6. Devereux J, Haeberli P & Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395.
7. Farkas T, Kiss J & Olasz F (1996) The construction and characterization of an effective transpositional system based on IS30. FEBS Lett 390: 53-58.
8. Feng X, Bednarz AL & Colloms SD (2010) Precise targeted integration by a chimaeric transposase zinc-finger fusion protein. Nucleic Acids Res 38: 1204-1216.
9. Gibson TJ (1984) Studies on the Epstein-Barr virus genome. PhD Thesis, Cambridge University, Cambridge.
10. Glasgow AC, Hughes KT & Simon MI (1989) Bacterial inversion systems. Mobile DNA (Berg DE & Howe MM, eds) pp. 637-660. ASM Press, Washington, DC.
11. Herrero M, de Lorenzo V & Timmis KN (1990) Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172: 6557-6567.
12. Imre A, Olasz F & Nagy B (2005) Development of a PCR system for the characterisation of Salmonella flagellin genes. Acta Vet Hung 53: 163-172.
13. Kiss J & Olasz F (1999) Formation and transposition of the covalently closed IS30 circle: the relation between tandem dimers and monomeric circles. Mol Microbiol 34: 37-52.
14. Kiss J, Szabo M & Olasz F (2003) Site-specific recombination by the DDE family member mobile element IS30 transposase. P Nat Acad Sci USA 100: 15000-15005.
15. Kolb AF, Coates CJ, Kaminski JM, Summers JB, Miller AD & Segal DJ (2005) Site-directed genome modification: nucleic acid and protein modules for targeted integration and gene correction. Trends Biotechnol 23: 399-406.
16. Macnab RM (1996) Flagella and motility. Escherichia coli and Salmonella Cellular and Molecular Biology (Neidhardt FC, ed), pp. 123-145. ASM Press, Washington, DC.
17. Nagy Z, Szabo M, Chandler M & Olasz F (2004) Analysis of the N-terminal DNA binding domain of the IS30 transposase. Mol Microbiol 54: 478-488.
18. Okazaki N, Matsuo S, Saito K, Tominaga A & Enomoto M (1993) Conversion of the Salmonella phase 1 flagellin gene fliC to the phase 2 gene fljB on the Escherichia coli K-12 chromosome. J Bacteriol 175: 758-766.
19. 2 and its role in IS30-mediated transpositional DNA rearrangements. Mol Gen Genet 239: 177-187.
20. Olasz F, Kiss J, Konig P, Buzas Z, Stalder R & Arber W (1998) Target specificity of insertion element IS30. Mol Microbiol 28: 691-704.
21. Parker CT & Guard-Petter J (2001) Contribution of flagella and invasion proteins to pathogenesis of Salmonella enterica serovar Enteritidis in chicks. FEMS Microbiol Lett 204: 287-291.
22. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Simon R, Priefer U & Pühler A (1983) A broad host range mobilization system for in vivo genetic engeneering: transposon mutagenesis in bacteria. Nat Biotechnol 1: 784-791.
24. Szabo M, Muller F, Kiss J, Balduf C, Strahle U & Olasz F (2003) Transposition and targeting of the prokaryotic mobile element IS30 in zebrafish. FEBS Lett 550: 46-50.
25. Szabo M, Kiss J, Nagy Z, Chandler M & Olasz F (2008) Sub-terminal sequences modulating IS30 transposition in vivo and in vitro. J Mol Biol 375: 337-352.
26. Voigt K, Izsvak Z & Ivics Z (2008) Targeted gene insertion for molecular medicine. J Mol Med 86: 1205-1219.