Interaction map of the Trypanosoma cruzi ribosomal P protein complex (stalk) and the elongation factor 2

Journal of Molecular Recognition

Volumn 24, Issue 2, 2011, Pages 359-370

  Smulski, Cristian R ^a,b   Longhi, Silvia A ^a   Ayub, Maximiliano Juri ^a,c   Edreira, Martin M ^a   Simonetti, Leandro ^a   Gõmez, Karina A ^a   Basile, Joaquín N ^a   Chaloin, Olivier ^b   Hoebeke, Johan ^b   Levin, Mariano J ^a  

^a INSTITUTO DE INVESTIGACIONES EN INGENIERÍA GENÉTICA Y BIOLOGÍA MOLECULAR   (Argentina)

^b INSTITUT DE BIOLOGIE MOLÉCULAIRE ET CELLULAIRE   (France)

^c UNIVERSIDAD NACIONAL DE SAN LUIS   (Argentina)

Author keywords

acidic ribosomal P proteins; elongation factor 2; protein protein interaction; stalk complex; surface plasmon resonance; Trypanosoma cruzi

Indexed keywords

ELONGATION FACTOR 2; MULTIPROTEIN COMPLEX; PROTOZOAL PROTEIN; RIBOSOME PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; GENE; GENETICS; KINETICS; METABOLISM; MOLECULAR GENETICS; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; SEQUENCE ANALYSIS; SURFACE PLASMON RESONANCE; TRYPANOSOMA CRUZI; TWO HYBRID SYSTEM;

AMINO ACID SEQUENCE; GENES, PROTOZOAN; KINETICS; MOLECULAR SEQUENCE DATA; MULTIPROTEIN COMPLEXES; PEPTIDE ELONGATION FACTOR 2; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; PROTOZOAN PROTEINS; RIBOSOMAL PROTEINS; SEQUENCE ANALYSIS, PROTEIN; SURFACE PLASMON RESONANCE; TRYPANOSOMA CRUZI; TWO-HYBRID SYSTEM TECHNIQUES;

EUKARYOTA; TRYPANOSOMA CRUZI;

EID: 79952151240     PISSN: 09523499     EISSN: 10991352     Source Type: Journal    
DOI: 10.1002/jmr.1089     Document Type: Article

References (29)

1. Aruna K, Chakraborty T, Rao PN, Santos C, Ballesta JPG, Sharma S,. 2005. Functional complementation of yeast ribosomal P0 protein with Plasmodium falciparum P0. Gene 357: 9-17. (Pubitemid 41267310)
2. Bargis-Surgey P, Lavergne JP, Gonzalo P, Vard C, Filhol-Cochet O, Reboud JP,. 1999. Interaction of elongation factor eEF-2 with ribosomal P proteins. Eur. J. Biochem. 262: 606-611. (Pubitemid 29266076)
3. Diaconu M, Kothe U, Schlünzen F, Fischer N, Harms JM, Tonevitsky AG, Stark H, Rodnina MV, Wahl MC,. 2005. Structural basis for the function of the ribosomal L7/12 stalk in factor binding and GTPase activation. Cell 121: 991-1004. (Pubitemid 40884392)
4. Gomez E, Medina G, Ballesta JPG, Levin MJ, Tellez-Iñon MT,. 2001. Acidic ribosomal P proteins are Phosphorylated in Trypanosoma cruzi. Int. J. Parasitol. 31: 1032-1039. (Pubitemid 32592448)
5. Gomez-Lorenzo MG, Spahn CM, Agrawal RK, Grassucci RA, Penczek P, Chakraburtty K, Ballesta JP, Lavandera JL, Garcia-Bustos JF, Frank J,. 2000. Three-dimensional cryo-electron microscopy localization of EF2 in the Saccharomyces cerevisiae 80S ribosome at 17.5 A resolution. EMBO J. 19: 2710-2718. (Pubitemid 30323559)
6. Gonzalo P, Lavergne JP, Reboud JP,. 2001. Pivotal role of the P1 N-terminal domain in the assembly of the mammalian ribosomal stalk and in the proteosynthetic activity. J. Biol. Chem. 276: 19762-19769.
7. Guarinos E, Remacha M, Ballesta JP,. 2001. Asymmetric interactions between the acidic P1 and P2 proteins in the Saccharomyces cerevisiae ribosomal stalk. J. Biol. Chem. 276: 32474-32479.
8. Hagiya A, Naganuma T, Maki Y, Ohta J, Tohkairin Y, Shimizu T, Nomura T, Hachimori A, Uchiumi T,. 2005. A mode of assembly of P0, P1, and P2 proteins at the GTPase-associated center in animal ribosome: in vitro analyses with P0 truncation mutants. J. Biol. Chem. 280: 39193-39199. (Pubitemid 41713871)
9. Janknecht R, de Martynoff G, Lou J, Hipskind RA, Nordheim A, Stunnenberg HG,. 1991. Rapid and efficient purification of native histidine-tagged protein expressed by recombinant vaccinia virus. Proc. Natl. Acad. Sci. USA 88: 8972-8976. (Pubitemid 21915552)
10. JÃrgensen R, Ortiz PA, Carr-Schmid A, Nissen P, Kinzy TG, Andersen GR,. 2003. Two crystal structures demonstrate large conformational changes in the eukaryotic ribosomal translocase. Nat. Struct. Biol. 10: 379-385. (Pubitemid 36523273)
11. Juri Ayub M, Smulski CR, Nyambega B, Bercovich N, Masiga D, Vazquez MP, Aguilar CF, Levin MJ,. 2005. Protein-protein interaction map of the Trypanosoma cruzi ribosomal P protein complex. Gene 357: 129-136. (Pubitemid 41297579)
12. Kelley LA, Sternberg MJE,. 2009. Protein structure prediction on the web: a case study using the Phyre server. Nat. Protoc. 4: 363-371.
13. Krokowski D, Boguszewska A, Abramczyk D, Liljas A, Tchõrzewski M, Grankowski N,. 2006. Yeast ribosomal P0 protein has two separate binding sites for P1/P2 proteins. Mol. Microbiol. 60: 386-400.
14. Lalioti VS, Pérez-Fernández J, Remacha M, Ballesta JP,. 2002. Characterization of interaction sites in the Saccharomyces cerevisiae ribosomal stalk components. Mol. Microbiol. 46: 719-729. (Pubitemid 35365607)
15. Levin MJ, Vazquez M, Kaplan D, Schijman AG,. 1993. The Trypanosoma cruzi ribosomal P protein family: classification and antigenicity. Parasitol. Today 9: 381-384. (Pubitemid 23278470)
16. Liljas A,. 1991. Comparative biochemistry and biophysics of ribosomal proteins. Int. Rev. Cytol. 124: 103-136.
17. MacConnell WP, Kaplan NO,. 1982. The activity of the acidic phosphoproteins from the 80 S rat liver ribosome. J. Biol. Chem. 257: 5359-5366.
18. Muller S, Couppez M, Briand JP, Gordon J, Sautiere P, Van Regenmortel MHV,. 1985. Antigenic structure of histone H2B. Biochim. Biophys. Acta 827: 235-246. (Pubitemid 15143612)
19. Naganuma T, Nomura N, Yao M, Mochizuki M, Uchiumi T, Tanaka I,. 2010. Structural basis for translation factor recruitment to the eukaryotic/archaeal ribosomes. J. Biol. Chem. 285: 4747-4756.
20. Perentesis JP, Phan LD, Gleason WB, LaPorte DC, Livingston DM, Bodley JW,. 1992. Saccharomyces cerevisiae elongation factor 2. Genetic cloning, characterization of expression and G-domain modeling. J. Biol. Chem. 267: 1190-1197.
21. Pérez-Fernández J, Remacha M, Ballesta JPG,. 2005. The acidic protein binding site is partially hidden in the free Saccharomyces cerevisiae ribosomal stalk protein P0. Biochemistry 44: 5532-5540. (Pubitemid 40489993)
22. Planta RJ, Mager WH,. 1998. The list of cytoplasmic ribosomal proteins of Saccharomyces cerevisiae. Yeast 14: 471-477. (Pubitemid 28138643)
23. Redpath NT, Price NT, Severinov KV, Proud CG,. 1993. Regulation of elongation factor-2 by multisite phosphorylation. Eur. J. Biochem. 213: 689-699. (Pubitemid 23125638)
24. Rodriguez-Gabriel MA, Remacha M, Ballesta JPG,. 2000. The RNA interacting domain but not the protein interacting domain is highly conserved in ribosomal protein P0. J. Biol. Chem. 275: 2130-2136. (Pubitemid 30060842)
25. Santos C, Ballesta JP,. 1995. The highly conserved protein P0 carboxyl end is essential for ribosome activity only in the absence of proteins P1 and P2. J. Biol. Chem. 270: 20608-20614.
26. Sprang SR,. 1997. G protein mechanisms: insights from structural analysis. Annu. Rev. Biochem. 66: 639-678. (Pubitemid 27274670)
27. Tsurugi K, Mitsui K,. 1991. Bilateral hydrophobic zipper as a hypothetical structure which binds acidic ribosomal protein family together on ribosomes in yeast Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 174: 1318-1323.
28. Uchiumi T, Kominami R,. 1997. Binding of mammalian ribosomal protein complex P0.P1.P2 and protein L12 to the GTPase-associated domain of 28 S ribosomal RNA and effect on the accessibility to anti-28 S RNA autoantibody. J. Biol. Chem. 272: 3302-3308.
29. Uchiumi T, Traut RR, Kominami R,. 1990. Monoclonal antibodies against acidic phosphoproteins P0, P1, and P2 of eukaryotic ribosomes as functional probes. J. Biol. Chem. 265: 89-95.