The lack of a nitrogen source and/or the C/N ratio affects the molecular weight of alginate and its productivity in submerged cultures of Azotobacter vinelandii

Annals of Microbiology

Volumn 60, Issue 4, 2010, Pages 661-668

  Zapata Vélez, Ana M ^a,b   Trujillo Roldán, Mauricio A ^a,c  

^a UNIVERSIDAD NACIONAL DE COLOMBIA   (Colombia)

^b Centro Nacional de Producción Más Limpia Y Tecnologías Ambientales   (Colombia)

^c UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

Author keywords

Alginate; Azotobacter vinelandii; Carbon nitrogen ratio; Molecular weight

Indexed keywords

AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS);

EID: 79952005355     PISSN: 15904261     EISSN: 18692044     Source Type: Journal    
DOI: 10.1007/s13213-010-0111-7     Document Type: Article

References (35)

1. Bhattacharya SK, Dubey AK (1997) High-level expression of a heterologous gene in Escherichia coli in response to carbon-nitrogen source and C/N ratio in a batch bioreactor. Biotechnol Prog 13:151-155 (Pubitemid 27169868)
2. Brivonese A, Sutherland WI (1989) Polymer production by a mucoid strain of Azotobacter vinelandii in batch culture. Appl Microbiol Biotechnol 30:97-102
3. Brownlee IA, Allen A, Pearson JP, Dettmar PW, Havler ME, Atherton MR, Onsoyen E (2005) Alginate as a source of dietary fiber. Crit Rev Food Sci Nutr 45:497-510 (Pubitemid 43203791)
4. Castañeda M, Guzman J, Moreno S, Espín G (2000) The GacS sensor kinase regulates alginate and poly-beta-hydroxybutyrate production in Azotobacter vinelandii. J Bacteriol 182:2624-2628
5. CastañedaM, Sanchez J, Moreno S, Nuñez C, Espin G (2001) The global regulators GacA and sigma(S) form part of a cascade that controls alginate production in Azotobacter vinelandii. J Bacteriol 183:6787-6793 (Pubitemid 33064191)
6. Chéze-Lange H, Beunard D, Dhulster P, Guillochon D, Cazé AM, Morcellet M, Saude N, Junter GA (2002) Production of microbial alginate in a membrane bioreactor. Enzyme Microb Technol 30:656-661 (Pubitemid 34327701)
7. Cho K, Ryu HW, Park C, Goodrich PR (2001) Utilization of swine wastewater as a feedstock for the production of polyhydroxyalkanoates by Azotobacter vinelandii UWD. J Biosci Bioeng 91:129-133 (Pubitemid 32226021)
8. Clementi F (1997) Alginate production by Azotobacter vinelandii. Crit Rev Biotechnol 17:327-361
9. Clementi F, Fantozzi P, Mancini F, Moresi M (1995) Optimal conditions for alginate production by Azotobacter vinelandii. Enzyme Microb Technol 17:983-988
10. Diaz-Barrera A, Peña C, Galindo E (2007) The oxygen transfer rate influences the molecular mass of the alginate produced by Azotobacter vinelandii. Appl Microbiol Biotechnol 76:903-910 (Pubitemid 47340940)
11. 2 transfer rate in chemostat cultures. Biotechnol Lett 31:825-829
12. Gacesa P (1988) Alginates. Carbohyd Polym 8:161-182
13. Gacesa P (1998) Bacterial alginate biosynthesis, recent progress and future prospects. Microbiology 144:1133-1143
14. Galindo E, Peña C, Nuñez C, Segura D, Espin G (2007) Molecular and bioengineering strategies to improve alginate and polydydroxyalkanoate production by Azotobacter vinelandii. Microb Cell Fact 6:7
15. Halbleib CM, Ludden PW (2000) Regulation of biological nitrogen fixation. J Nutr 130(5):1081-1084 (Pubitemid 30233250)
16. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426-428
17. Oelze J (2000) Respiratory protection of nitrogenase in Azotobacter species: is a widely held hypothesis unequivocally supported by experimental evidence? FEMS Microbiol Rev 24(4):321-333
18. Oppenheim J, Marcus L (1970) Correlation of ultrastructure in Azotobacter vinelandii with nitrogen source for growth. J Bacteriol 101(1):286-291
19. Orr D, Zheng W, Campbell BS, McDougall BM, Seviour RJ (2009) Culture conditions affect the chemical composition of the exopolysaccharide synthesized by the fungus Aureobasidium pullulans. J Appl Microbiol 107:691-698
20. Page WJ, Cornish A (1993) Growth of Azotobacter vinelandii UWD in fish peptone medium and simplified extraction of poly-beta-hydroxybutyrate. Appl Environ Microbiol 59:4236-4244 (Pubitemid 23361412)
21. Peña C, Campos N, Galindo E (1997) Changes in alginate molecular mass distributions, broth viscosity and morphology of Azotobacter vinelandii cultured in shake flasks. Appl Microbiol Biotechnol 48:510-515 (Pubitemid 27475290)
22. Peña C, Trujillo-Roldán MA, Galindo E (2000) Influence of dissolved oxygen tension and agitation speed on alginate production and its molecular weight in cultures of Azotobacter vinelandii. Enzyme Microb Technol 27:390-398 (Pubitemid 30628446)
23. Peña C, Hernandez L, Galindo E (2006) Manipulation of the acetylation degree of Azotobacter vinelandii alginate by supplementing the culture medium with 3-(N-morpholino)-propane-sulfonic acid. Lett Appl Microbiol 43:200-204 (Pubitemid 44050636)
24. Peña C, Peter CP, Büchs J, Galindo E (2007) Evolution of the specific power consumption and oxygen transfer rate in alginateproducing cultures of Azotobacter vinelandii conducted in shake flasks. Biochem Eng J 36:73-80 (Pubitemid 47268829)
25. Peña C, Millán M, Galindo E (2008) Production of alginate by Azotobacter vinelandii in a stirred fermentor simulating the evolution of power input observed in shake flasks. Process Biochem 43:775-778
26. Priego-Jimenéz R, Peña C, Ramírez OT, Galindo E (2005) Specific growth rate determines the molecular mass of the alginate produced by Azotobacter vinelandii. Biochem Eng J 25:187-193 (Pubitemid 41188193)
27. Pyla R, Kim TJ, Silva JL, Jung YS (2009) Overproduction of poly-beta-hydroxybutyrate in the Azotobacter vinelandii mutant that does not express small RNA ArrF. Appl Microbiol Biotechnol 84:717-724
28. Rehm BH, Valla S (1997) Bacterial alginates: biosynthesis and applications. Appl Microbiol Biotechnol 48:281-288 (Pubitemid 27430075)
29. Sabra W, Zeng AP, Lunsdorf H, Deckwer WD (2000) Effect of oxygen on formation and structure of Azotobacter vinelandii alginate and its role in protecting nitrogenase. Appl Environ Microbiol 66:4037-4044
30. Sabra W, Zeng AP, Deckwer WD (2001) Bacterial alginate: physiology, product quality and process aspects. Appl Microbiol Biotechnol 56:315-325 (Pubitemid 32768594)
31. Sabry SA, Ghanem KM, Sabra WA (1996) Effect of nutrients on alginate synthesis in Azotobacter vinelandii and characterization of the produced alginate. Microbiology 12:593-598
32. Trujillo-Roldán MA, Peña C, Ramirez OT, Galindo E (2001) Effect of oscillating dissolved oxygen tension on the production of alginate by Azotobacter vinelandii. Biotechnol Prog 17:1042-1048 (Pubitemid 34018282)
33. Trujillo-Roldán MA, Moreno S, Segura D, Galindo E, Espin G (2003a) Alginate production by an Azotobacter vinelandii mutant unable to produce alginate lyase. Appl Microbiol Biotechnol 60:733-737 (Pubitemid 36286682)
34. Trujillo-Roldán MA, Peña C, Galindo E (2003b) Components in the inoculum determine the kinetics of Azotobacter vinelandii cultures and the molecular weight of its alginate. Biotechnol Lett 25:1251-1254 (Pubitemid 37053885)
35. Trujillo-Roldán MA, Moreno S, Espin G, Galindo E (2004) The roles of oxygen and alginate-lyase in determining the molecular weight of alginate produced by Azotobacter vinelandii. Appl Microbiol Biotechnol 63:742-747 (Pubitemid 38316703)