High-level expression of a heterologous gene in Escherichia coli in response to carbon-nitrogen source and C/N ratio in a batch bioreactor

Biotechnology Progress

Volumn 13, Issue 2, 1997, Pages 151-155

  Bhattacharya, Sanjoy K ^a   Dubey, Ashok K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; NITROGEN;

ARTICLE; BIOREACTOR; BIOTECHNOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; METABOLISM; METHODOLOGY; PH; TEMPERATURE;

BIOREACTORS; BIOTECHNOLOGY; CARBON; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; NITROGEN; TEMPERATURE;

EID: 0031104704     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp970007t     Document Type: Article

References (35)

1. Aiba, S.; Koizumi, J. Effects of Temperature on Plasmid Stability and Penicillinase Productivity in a Transformant Bacillus stearothermophillus. Biotechnol. Bioeng. 1984, 26, 1026-1031.
2. Bhattacharya. S. K.; Dubey, A. K. Metabolic burden as reflected by maintenance coefficient of recombinant Escherichia coli overexpressing target gene. Biotechnol. Lett. 1995, 17, 1155-1160.
3. Bhattacharya, S. K.; Dubey, A. K. Expression parameters for target gene cloned in Escherichia coli in response to phosphate supply. Biotechnol. Lett. 1996, 18, 1145-1148.
4. Bhattacharya, S. K.; Dubey, A. K. Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli. Enzyme Microb. Technol. 1997, 20, in press.
5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
6. Chalmers, J. J. et al. Effects of temperature on Escherichia coli overproducing β-Lactamase or human epidermal growth factor. Appl. Environ. Microbiol. 1990, 56, 104-111.
7. Chou-H.; Bennett, G. N.; San-Y. Effect of modulated glucose uptake on high level recombinant protein production in a dense Escherichia coli Culture. Biotechnol. Prog. 1994, 10, 644-647.
8. Dubey, A. K.; Mukhopadhaya, S. N.; Visaria, V. S.; Ghose, T. K. Sources, production and purification of restriction enzymes. Process Biochem. 1987, 22, 25-34.
9. Dubey, A. K.; Mollet, B.; Roberts, R. J. Purification and Characterization of MspI DNA methyltransferase cloned and overexpressed in E. coli. Nucleic Acids Res. 1992, 20, 1579-1585.
10. George, H. A.; Powell, A. L.; Dahlgren, M. E.; Herber, W. K.; Maignetter, R. Z.; Burgess, B. W.; Stirdivant, S. M.; Greasham, R. L. Physiological effects of TGFα-PE40 expression in recombinant Escherichia coli JM 109. Biotechnol. Bioeng. 1992, 40, 437-445.
11. Ingraham, J. L.; Maaloe, O.; Neidhart, F. C. Growth of the bacterial Cell; Sinauer Associates Inc.: Sunderland, MA, 1983.
12. Jensen, E. B.; Carlsen, S. Production of recombinant human growth hormone in Escherichia coli.: Expression of differential precursors and physiological effects of glucose, acetate and salt. Biotechnol. Bioeng. 1990, 36, 1-11.
13. Kakizono, T.; Kobayashi, M.; Magai, S. Effect of carbon nitrogen ratio on encystment accompanied with Astaxanthin formation in green alga Haemetococcus pluvialis. J. Ferment. Bioeng. 1992, 74, 403-405.
14. Klimasauskas, S.; Nelson, J. L.; Roberts, R. J. The sequence specifictiy domain of cytosine-C5 methylase. Nucleic Acids Res. 1991, 19, 6183-6190.
15. Kwon, S.; Kim, S.; Kim, E. Effect of glycerol on β-Lactamase production during high cell density cultivation of recombinant Escherichia coli. Biotechnol. Prog. 1996, 12, 205-208.
16. Ladish, M. L.; Kohlmann, K. L. Recombinant human Insulin. Biotechnol. Prog. 1992, 8, 469-478.
17. Lee, S. Y.; Lee, Y. K.; Chang, H. N. Stimulating effects of amino acids and oleic acid on poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli. J. Ferment. Bioeng. 1995, 79, 177-180.
18. Li, X.; Taylor, K. B. Effect of glucose on the expression parameters of recombinant proteins on Escherichia coli during batch growth in complex medium. Biotechnol. Prog. 1994, 10, 160-164.
19. Lin, P. M.; Lee, C. H.; Roberts, R. J. Cloning and characterization of the genes encoding the MspI restriction-modification system. Nucleic Acids Res. 1989, 17, 301-3011.
20. Mi, S.; Roberts, R. J. How M.MspI and M.HpaII decide which base to methylate. Nucleic Acids Res. 1992, 20, 4811-4816.
21. Miller, J. H. Experiments in Molecular Genetics; Cold Spring Harbor Laboratory Press: New York, 1972.
22. Old, R. W.; Primrose, S. B. Principles of gene manipulation; Blackwell Scientific Publications: Oxford, U.K., 1989.
23. Pilon, A. L.; Yost, P.; Chase, T. E.; Lohnas, G. L; Bentley, W. E. High-level expression and efficient recovery of ubiquitin fusion proteins from Escherichia coli. Biotechnol. Prog. 1996, 12, 331-337.
24. Pirt, S. J. Principles of Microbe and Cell Cultivation; Blackwell Scientific Publications, Oxford, U.K., 1975.
25. Projan, S. J.; Carleion, S.; Novick, R. P. Determination of plasmid copy number by fluoresence densitometry. Plasmid 1983, 9, 182-190.
26. Rinas, U.; Kacke-Helm, H. A.; Schugerl, K. Glucose as a substrate in recombinant strain fermentation technology. By product formation, degradation, and intracellular accumulation of recombinant protein. Appl. Microbial Biotechnol. 1989, 31, 163-167.
27. Ryan, W.; Parulekar, S. J.; Stark, B. C. Expression of β-lactamase by recombinant Escherichia coli strains containing plasmids of different sizes-effects of pH, phosphate and dissolved oxygen. Biotechnol. Bioeng. 1989, 34, 309-319.
28. Ryan, W.; Parulekar, S. J. Effects of culture conditions on plasmid stability and production of a plasmid-encoded protein in batch and continuous cultures of Escherichia coli JM103 [pUC108]. Ann. N. Y. Acad. Sci. 1990, 589, 91-110.
29. Shuler, M. L.; Kargi, F. Bioprocess Engineering; Prentice-Hall: New Jersey, 1992.
30. Tortora, G. J.; Funk, B. R.; Chase, C. L. Microbiology: An Introduction; Benjamin/Cummings Publishing Co. Inc.: Massachusetts, 1995.
31. Umemura, K.; Atomi, H.; Kanai, T.; Teranishi, Y.; Ueda, M.: Tanaka, A. Effects of carbon source on the application of a novel foreign gene expression system in Saccharomyces cerevisae using the upstream region of the Candida tropicalis Isocitrate lyase gene (UPR-ICL). J. Ferment. Bioeng. 1995, 80, 529-533.
32. Vila, P.; Villeverde, A. Inhibition of cI857-controlled recombinant gene expression in Escherichia coli at very low concentrations of glucose. Biotechnol. Lett. 1993, 15, 549-552.
33. Vila, P.; Corchero, J. L.; Benito, A.; Villaverde, A. Ammonium-mediated reduction of plasmid copy number and recombinant gene expression in Escherichia coli. Biotechnol. Prog. 1994, 10, 648-651.
34. Williams, D. C.; VanFrank, R. M.; Muth, W. L.; Burnett, J. P. Science 1982, 215, 687-688.
35. Wu, J. C.; Santi, D. V. High level expression and purification of HhaI methyltransferase. Nucleic Acids Res. 1988, 16, 703-717.