Fluorescent cell barcoding for multiplex flow cytometry

Current Protocols in Cytometry

Volumn , Issue SUPPL.55, 2011, Pages

  Krutzik, Peter O ^a   Clutter, Matthew R ^a   Trejo, Angelica ^a   Nolan, Garry P ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Barcode; Dye; Flow cytometry; Fluorescence; High throughput; Multiplex

Indexed keywords

BUFFER; FLUORESCENT DYE;

ANALYTICAL ERROR; ARTICLE; CELL LABELING; CELL LINE; COMPUTER PROGRAM; CONCENTRATION (PARAMETERS); CYTOCHEMISTRY; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENT CELL BARCODING; HIGH THROUGHPUT SCREENING; MULTIPLEX FLOW CYTOMETRY; PRIORITY JOURNAL; PROCEDURES CONCERNING CELLS;

EID: 79951918942     PISSN: 19349297     EISSN: 19349300     Source Type: Journal    
DOI: 10.1002/0471142956.cy0631s55     Document Type: Article

References (6)

1. Clutter, M.R., Heffner, G.C., Krutzik, P.O., Sachen, K.L., and Nolan, G.P. 2010. Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry. Cytometry A 77:1020-1031.
2. Irish, J.M., Myklebust, J.H., Alizadeh, A.A., Houot, R., Sharman, J.P., Czerwinski, D.K., Nolan, G.P., and Levy, R. 2010. B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression. Proc. Natl. Acad. Sci. U.S.A. 107:12747-12754.
3. Krutzik, P.O. and Nolan, G.P. 2003. Intracellular phospho-protein staining techniques for flow cytometry: monitoring single cell signaling events. Cytometry A 55:61-70.
4. Krutzik, P.O. and Nolan, G.P. 2006. Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling. Nat. Methods 3:361-368.
5. Krutzik, P.O., Crane, J.M., Clutter, M.R., and Nolan, G.P. 2008. High-content single-cell drug screening with phosphospecific flow cytometry. Nat. Chem. Biol. 4:132-142.
6. Schulz, K.R., Danna, E.A., Krutzik, P.O., and Nolan, G.P. 2007. Single-cell phospho-protein analysis by flow cytometry. Curr. Protoc. Immunol. 78:Unit 8.17.1-8.17.20.