Efficient insertional mutagenesis system for the dimorphic pathogenic fungus Sporothrix schenckii using Agrobacterium tumefaciens

Journal of Microbiological Methods

Volumn 84, Issue 3, 2011, Pages 418-422

  Zhang, Yanhua ^a,b   Li, Guangquan ^a   He, Dan ^a   Yu, Baodong ^b   Yokoyama, Koji ^c   Wang, Li ^a  

^a Basic Medical College   (China)

^b JILIN UNIVERSITY   (China)

^c CHIBA UNIVERSITY   (Japan)

Author keywords

Agrobacterium tumefaciens; Dimorphic fungus; Sporothrix schenckii; T DNA flanking sequences; Transformation

Indexed keywords

FUNGAL DNA;

ARTICLE; BACTERIUM TRANSFORMATION; CONIDIUM; CONTROLLED STUDY; DNA SEQUENCE; FUNGAL STRAIN; FUNGUS MUTANT; GENE EXPRESSION REGULATION; MINIMUM INHIBITORY CONCENTRATION; MITOSIS; NONHUMAN; PHENOTYPE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RHIZOBIUM RADIOBACTER; SPOROTHRIX SCHENCKII;

ANIMALS; GENETICS, MICROBIAL; HUMANS; MUTAGENESIS, INSERTIONAL; RHIZOBIUM RADIOBACTER; SPOROTHRIX; TRANSFORMATION, GENETIC; UNITED STATES;

AGROBACTERIUM TUMEFACIENS; ANIMALIA; FUNGI; SPOROTHRIX SCHENCKII;

EID: 79951811999     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.01.017     Document Type: Article

References (29)

1. Combier J.P., Melayah D., Raffier C., Gay G., Marmeisse R. Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the symbiotic ectomycorrhizal fungus Hebeloma cylindrosporum. FEMS Microbiol. Lett. 2003, 220:141-148.
2. dos Reis M.C., Pelegrinelli Fungaro M.H., Delgado Duarte R.T., Furlaneto L., Furlaneto M.C. Agrobacterium tumefaciens-mediated genetic transformation of the entomopathogenic fungus Beauveria bassiana. J. Microbiol. Methods 2004, 58:197-202.
3. Erster O., Liscovitch M. A modified inverse PCR procedure for insertion, deletion, or replacement of a DNA fragment in a target sequence and its application in the ligand interaction scan method for generation of ligand-regulated proteins. Methods Mol. Biol. 2010, 634:157-174.
4. Figueiredo J.G., Goulin E.H., Tanaka F., Stringari D., Kava-Cordeiro V., Galli-Terasawa L.V., Staats C.C., Schrank A., Glienke C. Agrobacterium tumefaciens-mediated transformation of Guignardia citricarpa. J. Microbiol. Methods 2010, 80:143-147.
5. Heidekamp F., Dirkse W.G., Hille J., van Ormondt H. Nucleotide sequence of the Agrobacterium tumefaciens octopine Ti plasmid-encoded tmr gene. Nucleic Acids Res. 1983, 11:6211-6223.
6. Huser A., Takahara H., Schmalenbach W., O'Connell R. Discovery of pathogenicity genes in the crucifer anthracnose fungus Colletotrichum higginsianum, using random insertional mutagenesis. Mol. Plant Microbe Interact. 2009, 22:143-156.
7. Isabel M.M., Melissa O.X., Antonella S.M., Cristina G.F., Thomaz N.G., Rosema S., Luiz F.D.S., Marcia O.N., Mario C.A.M. Role of melanin in the pathogenesis of cutaneous sporotrichosis. Microbes Infect. 2010, 12:162-165.
8. Jeon J., Park S.Y., Chi M.H., Choi J., Park J., Rho H.S., Kim S., Goh J., Yoo S., Park J.Y., Yi M., Yang S., Kwon M.J., Han S.S., Kim B.R., Khang C.H., Park B., Lim S.E., Jung K., Kong S., Karunakaran M., Oh H.S., Kim H., Kang S., Choi W.B., Lee Y.H. Genome-wide functional analysis of pathogenicity genes in the rice blast fungus. Nat. Genet. 2007, 39:561-565.
9. Jeon J.S., Lee S., Jung K.H., Jun S.H., Jeong D.H., Lee J., Kim C., Jang S., Yang K., Nam J., An K., Han M.J., Sung R.J., Choi H.S., Yu J.H., Choi J.H., Cho S.Y., Cha S.S., Kim S.I., An G. T-DNA insertional mutagenesis for functional genomics in rice. Plant J. 2000, 22:561-570.
10. Koukolikova-Nicola Z., Hohn B. How does the T-DNA of Agrobacterium tumefaciens find its way into the plant cell nucleus?. Biochimie 1993, 75:635-638.
11. Kummasook A., Cooper C.R., Vanittanakom N. An improved Agrobacterium-mediated transformation system for the functional genetic analysis of Penicillium marneffei. Med. Mycol. 2010, online.
12. Lai E.M., Shih H.W., Wen S.R., Cheng M.W., Hwang H.H., Chiu S.H. Proteomic analysis of Agrobacterium tumefaciens response to the Vir gene inducer acetosyringone. Proteomics 2006, 6:4130-4136.
13. Lazo G.R., Stein P.A., Ludwig R.A. A DNA transformation-competent Arabidopsis genomic library in Agrobacterium. Biotechnology (N Y) 1991, 9:963-967.
14. Leclerque A., Wan H., Abschutz A., Chen S., Mitina G.V., Zimmermann G., Schairer H.U. Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana. Curr. Genet. 2004, 45:111-119.
15. Lopes-Bezerra L.M., Schubach A., Costa R.O. Sporothrix schenckii and sporotrichosis. An. Acad. Bras. Ciênc. 2006, 78:293-308.
16. Machida Y., Yoshioka Y. Mechanism of T-DNA integration into plant genomes and trial of plant gene targeting. Tanpakushitsu Kakusan Koso 1992, 37:1041-1046.
17. Michielse C.B., Hooykaas P.J., van den Hondel C.A., Ram A.F. Agrobacterium-mediated transformation of the filamentous fungus Aspergillus awamori. Nat. Protoc. 2008, 3:1671-1678.
18. Michielse C.B., van Wijk R., Reijnen L., Cornelissen B.J., Rep M. Insight into the molecular requirements for pathogenicity of Fusarium oxysporum f. sp. lycopersici through large-scale insertional mutagenesis. Genome Biol. 2009, 10:R4.
19. Mullins E.D., Chen X., Romaine P., Raina R., Geiser D.M., Kang S. Agrobacterium-mediated transformation of Fusarium oxysporum: an efficient tool for insertional mutagenesis and gene transfer. Phytopathology 2001, 91:173-180.
20. O'Malley R.C., Alonso J.M., Kim C.J., Leisse T.J., Ecker J.R. An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome. Nat. Protoc. 2007, 2:2910-2917.
21. Rho H.S., Kang S., Lee Y.H. Agrobacterium tumefaciens-mediated transformation of the plant pathogenic fungus. Magnaporthe grisea. Mol. Cells 2001, 12:407-411.
22. Silva-Vergara M.L., Maneira F.R., De Oliveira R.M., Santos C.T., Etchebehere R.M., Adad S.J. Multifocal sporotrichosis with meningeal involvement in a patient with AIDS. Med. Mycol. 2005, 43:187-190.
23. Singer T., Burke E. High-throughput TAIL-PCR as a tool to identify DNA flanking insertions. Methods Mol. Biol. 2003, 236:241-272.
24. Stachel S.E., Timmerman B., Zambryski P. Activation of Agrobacterium tumefaciens vir gene expression generates multiple single-stranded T-strand molecules from the pTiA6 T-region: requirement for 5' virD gene products. EMBO J. 1987, 6:857-863.
25. Talhinhas P., Muthumeenakshi S., Neves-Martins J., Oliveira H., Sreenivasaprasad S. Agrobacterium-mediated transformation and insertional mutagenesis in Colletotrichum acutatum for investigating varied pathogenicity lifestyles. Mol. Biotechnol. 2008, 39:57-67.
26. Wang L., Yokoyama K., Miyaji M., Nishimura K. The identification and phylogenetic relationship of pathogenic species of Aspergillus based on the mitochondrial cytochrome b gene. Med. Mycol. 1998, 36:153-164.
27. Wirawan I.G., Kang H.W., Kojima M. Isolation and characterization of a new chromosomal virulence gene of Agrobacterium tumefaciens. J. Bacteriol. 1993, 175:3208-3212.
28. Zhang P., Xu B., Wang Y., Li Y., Qian Z., Tang S., Huan S., Ren S. Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in the fungus Penicillium marneffei. Mycol. Res. 2008, 112:943-949.
29. Zhong Y.H., Wang X.L., Wang T.H., Jiang Q. Agrobacterium-mediated transformation (AMT) of Trichoderma reesei as an efficient tool for random insertional mutagenesis. Appl. Microbiol. Biotechnol. 2007, 73:1348-1354.