Agrobacterium-mediated transformation of Fusarium oxysporum: An efficient tool for insertional mutagenesis and gene transfer

Phytopathology

Volumn 91, Issue 2, 2001, Pages 173-180

  Mullins, E D ^a   Chen, X ^a   Romaine, P ^a   Raina, R ^a   Geiser, D M ^a   Kang, S ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOSYRINGONE; ANTIBIOTIC RESISTANCE; BACTERIAL CELL; BINARY VECTOR; CELL GROWTH; COCULTURE; DNA FLANKING REGION; DNA TRANSFER; FUNGUS SPORE; GENE TRANSFER; GENETIC MANIPULATION; GENETIC TRANSFORMATION; INSERTIONAL MUTAGENESIS;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ASPERGILLUS; ASPERGILLUS NIDULANS; BACTERIA (MICROORGANISMS); EMERICELLA NIDULANS; FUNGI; FUSARIUM; FUSARIUM OXYSPORUM; RADIOBACTER; RHIZOBIUM;

EID: 0035136139     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO.2001.91.2.173     Document Type: Article

References (37)

1. AAL-toxin deficient mutants of Alternaria alternata tomato pathotype by restriction enzyme-mediated integration
2. Identification of pathogenicity mutants of the rice blast fungus Magnaporthe grisea by insertional mutagenesis
3. Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI)
4. Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae
5. T-DNA from Agrobacterium tumefaciens as an efficient tool for gene targeting in Kluyveromyces lactis
6. Improved vectors for selecting resistance to hygromycin
7. Genetic and molecular characterization of embryonic mutants identified following seed transformation in Arabidopsis
8. Agrobacterium tumefaciens-mediated transformation of filamentous fungi
9. Cloning genes from T-DNA tagged mutants
10. Agrobacterium T-DNA: A silver bullet for filamentous fungi?
11. Transformation-mediated developmental mutants of Glomerella graminicola (Collectotrichum graminicola)
12. T-DNA insertion mutagenesis in Arabidopsis: Mutational spectrum
13. Carnation leaves as a substrate and for preserving cultures of Fusarium species
14. A homologous and self-replicating system for efficient transformation of Fusarium oxysporum
15. Transformation of Aspergillus awamori by Agrobacterium tumefaciens-mediated homologous recombination
16. Restriction enzyme-mediated DNA integration in Coprinus cinereus
17. Regulation of the transfer of Ti-plasmids of Agrobacterium tumefaciens
18. Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations
19. Genetic transformation of the fungal plant wilt pathogen Fusarium oxysporum
20. T-DNA insertional mutagenesis in Arabidopsis
21. T-DNA as an insertional mutagen in Arabidopsis
22. Influence of biological parameters and gene transfer technique on transformation of Fusarium oxysporum
23. Deletions in the gibberellin biosynthesis gene cluster of Gibberella fujikuroi by restriction enzyme-mediated integration and conventional transformation-mediated mutagenesis
24. Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR
25. Thermal asymmetric interlaced PCR: Automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking
26. Tagged mutations at the Tox1 locus of Cochliobolus heterostrophus by restriction enzyme-mediated integration
27. Direct sequencing by thermal asymmetric PCR
28. Stability of transformed antagonistic Fusarium oxysporum strains in vitro and in soil microcosms
29. Phylogenetic relationships among ascomycetous truffles and the true and false morels from 18S and 28S ribosomal DNA sequence analysis
30. Conversion of the pathogenic fungus Colletotrichum magna to a nonpathogenic endophytic mutualist by gene disruption
31. Increased transformation frequency and tagging of developmental genes in Aspergillus nidulans by restriction enzyme-mediated integration (REMI)
32. Genetic analysis of sporulation in Magnaporthe grisea by chemical and insertional mutagenesis
33. Use of a bacterial hygromycin B resistance gene as a dominant selectable marker in Neurospora crassa transformation
34. Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis
35. A miniprep procedure for isolating genomic DNA from Magnaporthe grisea
36. Potential application of genetic transformants of Fusarium oxysporum f. sp. raphani for assessing fungal autecology
37. REMI-induced mutants of Mycosphaerella zeae-maydis lacking the PM-toxin are deficient in pathogenesis to corn