Cloning and analysis of the xylAB operon and characterization of xylose isomerase from Thermoanaerobacter ethanolicus

Biotechnology Letters

Volumn 33, Issue 3, 2011, Pages 593-598

  Fan, Lijuan ^a   Zhang, Yongchang ^a   Qu, Wei ^a   Wang, Jingkai ^a   Shao, Weilan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

Characterization; Expression; Purification; Thermoanaerobacter ethanolicus; Xylose isomerase; Xylulokinase

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CLONE CELLS; CLONING; ENCODING (SYMBOLS); ENZYME ACTIVITY; ESCHERICHIA COLI; GENES; ION CHROMATOGRAPHY; PURIFICATION;

EXPRESSION; HALF LIVES; HYPOTHETICAL PROTEIN; ION EXCHANGE CHROMATOGRAPHY; THERMOANAEROBACTER; XYLOSE ISOMERASE; XYLULOKINASE;

ION EXCHANGE;

ESCHERICHIA COLI; THERMOANAEROBACTER; THERMOANAEROBACTER ETHANOLICUS;

EID: 79951770564     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0463-x     Document Type: Article

References (11)

1. Bhosale SH, Rao MB, Deshpande VV (1996) Molecular and industrial aspects of glucose isomerase. Microbiol Rev 60: 280-300.
2. Chauthaiwale J, Rao M (1994) Production and purification of extracellular d-xylose isomerase from an alkaliphilic, thermophilic Bacillus sp. Appl Environ Microbiol 60: 4495-4499.
3. Dische Z, Borenfreund E (1951) A new spectrophotometric method for the detection and determination of keto sugars and trioses. J Biol Chem 192: 583-587.
4. Feldmann SD, Sahm H, Sprenger GA (1992) Cloning and expression of the genes for xylose isomerase and xylulokinase from Klebsiella pneumoniae 1033 in Escherichia coli K12. Mol Gen Genet 234: 201-210.
5. Hahn-Hagerdal B, Wahlbom CF, Gardonyi M, van Zyl WH, Cordero Otero RR, Jonsson LJ (2001) Metabolic engineering of Saccharomyces cerevisiae for xylose utilization. Adv Biochem Eng Biotechnol 73: 53-84.
6. Jiang Y, Pei J, Song X, Shao W (2007) Restriction site-dependent PCR: an efficient technique for fast cloning of new genes of microorganisms. DNA Res 14: 283-290.
7. Kim BC, Yu SN, Kim KY, Lee JS, Pyun YR, Ahn SC (2010) Cloning, expression and characterization of xylose isomerase, XylA, from Caldanaerobactersubterraneus subsp. yonseiensis. Biotechnol Lett 32: 929-933.
8. Lee YE, Ramesh MV, Zeikus JG (1993) Cloning, sequencing and biochemical characterization of xylose isomerase from Thermoanaerobacterium saccharolyticum strain B6A-RI. J Gen Microbiol 139: 1227-1234.
9. Liu SY, Wiegel J, Gherardini FC (1996) Purification and cloning of a thermostable xylose (glucose) isomerase with an acidic pH optimum from Thermoanaerobacterium strain JW/SL-YS 489. J Bacteriol 178: 5938-5945.
10. Wiegel J, Ljungdahl LG (1981) Thermoanaerobacter ethanolicus gen. nov., spec. nov., a new, extreme thermophilic, anaerobic bacterium. Arch Microbiol 128: 343-348.
11. Wu H, Pei J, Jiang Y, Song X, Shao W (2010) pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes. Biotechnol Lett 32: 795-801.