pHsh vectors, a novel expression system of Escherichia coli for the large-scale production of recombinant enzymes

Biotechnology Letters

Volumn 32, Issue 6, 2010, Pages 795-801

  Wu, Huawei ^a,b   Pei, Jianjun ^a   Jiang, Yu ^c   Song, Xin ^c   Shao, Weilan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

^b YANGTZE UNIVERSITY   (China)

^c INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

Author keywords

Heat shock induction; Hsh system; Sigma 32

Indexed keywords

E. COLI; ENZYME PRODUCTION; EXPRESSION SYSTEM; FOREIGN GENES; HEAT-SHOCK; INDUCIBLE PROMOTER; LARGE-SCALE PRODUCTION; RECOMBINANT ENZYMES; SIGMA FACTOR; XYLANASES;

ENCODING (SYMBOLS); ENZYMES; ESCHERICHIA COLI; GENE ENCODING; SYNTHESIS (CHEMICAL);

GENE EXPRESSION;

ALPHA N ARABINOFURANOSIDASE; ALPHA-N-ARABINOFURANOSIDASE; ENDO 1,4 BETA XYLANASE; GLYCOSIDASE; HEAT SHOCK PROTEIN; HEAT SHOCK SIGMA FACTOR 32; HEAT-SHOCK SIGMA FACTOR 32; RECOMBINANT PROTEIN; SIGMA FACTOR;

ARTICLE; BIOSYNTHESIS; BIOTECHNOLOGY; DNA REPLICATION ORIGIN; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; METABOLISM; METHODOLOGY; PROMOTER REGION;

BIOTECHNOLOGY; ENDO-1,4-BETA XYLANASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; GLYCOSIDE HYDROLASES; HEAT-SHOCK PROTEINS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; REPLICATION ORIGIN; SIGMA FACTOR;

ESCHERICHIA COLI;

EID: 77952240448     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0223-y     Document Type: Article

References (14)

1. Amann E, Brosius J, Ptashne M (1983) Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli. Gene 25: 167-178.
2. Arsene F, Tomoyasu T, Bukau B (2000) The heat-shock response of Escherichia coli. Int J Food Microbiol 55: 3-9.
3. L to promote gene expression in hybrid plasmid cloning vehicles. Methods Enzymol 68: 482-492.
4. Binz T, Canevascini G (1996) Xylanases from the Dutch elm disease pathogens Ophiostoma ulmi and Ophiostoma novo-ulmi. Physiol Mol Plant Pathol 49: 159-175.
5. Calendar R, Erickson JW, Halling C, Nolte A (1988) Deletion and insertion mutations in the rpoH gene of Escherichia coli that produce functional sigma 32. J Bacteriol 150: 815-825.
6. Cowing DW, Bardwell JC, Craig EA, Woolford C, Hendrix RW, Gross CA (1985) Consensus sequence for Escherichia coli heat-shock gene promoters. Proc Natl Acad Sci USA 82: 2679-2683.
7. Gold L (1990) Expression of heterologous proteins in Escherichia coli. Methods Enzymol 185: 11-14.
8. Miller JH (1992) A short course in bacterial genetics (Handbook). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA.
9. L promoter of coliphage lambda. Gene 15: 81-93.
10. Shao W, Pei J, Li X (2005) The method for over-expression of the xylanase B from Thermotoga maritima in Escherichia coli. China Patent, ZL 03131524. 0.
11. Studier FW, Moffatt BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189: 113-130.
12. Tilly K, Spence J, Georgopoulos C (1989) Modulation of stability of the Escherichia coli heat-shock regulatory factor sigma. J Bacteriol 171: 1585-1589.
13. Wu H, Pei J, Wu G, Shao W (2008) Overexpression of GH10 endoxylanase XynB from T. maritima in E. coli by a novel vector with potential for industrial application. Enzyme Microb Technol 42: 230-234.
14. Xue Y, Wu A, Zeng H, Shao W (2006) High-level expression of an α-l-arabinofuranosidase from Thermotoga maritima in Escherichia coli for the production of xylobiose from xylan. Biotechnol Lett 28: 351-356.