Recovery of bulky DNA adducts by the regular and a modified 32P-postlabelling assay; influence of the DNA-isolation method

Mutation Research - Genetic Toxicology and Environmental Mutagenesis

Volumn 721, Issue 1, 2011, Pages 95-100

  Kovács, Katalin ^a   Anna, Lívia ^a   Rudnai, Péter ^a   Schoket, Bernadette ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH   (Hungary)

Author keywords

32P postlabelling; Bulky DNA adduct; DNA isolation methods; Phenol extraction; Qiagen kit; Throughput

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE P 32 GAMMA; BENZO[A]PYRENE; ENZYME; PHENOL; PHOSPHORUS 32; RADIOISOTOPE; SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; BIOASSAY; BLOOD BUFFY COAT; CELL STRAIN MCF 7; DNA ADDUCT; DNA ISOLATION; ENZYME SUBSTRATE; EVAPORATION; EXTRACTION; HUMAN; HUMAN CELL; HUMAN TISSUE; ISOTOPE LABELING; LUNG INJURY; LUNG PARENCHYMA; PERIPHERAL LYMPHOCYTE; PRIORITY JOURNAL; RADIOACTIVITY;

CELL LINE; DNA; DNA ADDUCTS; HUMANS; ISOTOPE LABELING; LUNG; LYMPHOCYTES; PHENOL; PHOSPHORUS RADIOISOTOPES; REAGENT KITS, DIAGNOSTIC;

EID: 79951674647     PISSN: 13835718     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mrgentox.2010.12.012     Document Type: Article

References (20)

1. Phillips D.H., Castegnaro M. Standardization and validation of DNA adduct postlabelling methods: report of interlaboratory trials and production of recommended protocols. Mutagenesis 1999, 14:301-315.
2. Segerbäck D., Vodicka P. Recoveries of DNA adducts of polycyclic aromatic hydrocarbons in the 32P-postlabelling assay. Carcinogenesis 1993, 14:2463-2469.
3. Szyfter K., Krüger J., Ericson P., Vaca C., Försti A., Hemminki K. 32P-Postlabelling analysis of DNA adducts in humans: adduct distribution and method improvement. Mutat. Res. 1994, 313:269-276.
4. Munnia A., Saletta F., Allione A., Piro S., Confortini M., Matullo G., Peluso M. 32P-Post-labelling method improvements for aromatic compound-related molecular epidemiology studies. Mutagenesis 2007, 22:381-385.
5. Godschalk R.W.L., Maas L.M., Kleinjans J.C.S., van Schooten F.J. Influences of DNA isolation and RNA contamination on carcinogen-DNA adduct analysis by 32P-postlabeling. Environ. Mol. Mutagen. 1998, 32:344-350.
6. Godschalk R., Nair J., van Schooten F.J., Risch A., Drings P., Kayser K., Dienemann H., Bartsch H. Comparison of multiple DNA adduct types in tumor adjacent human lung tissue: effect of cigarette smoking. Carcinogenesis 2002, 23:2081-2086.
7. Nair J., Gansauge F., Beger H., Dolara P., Winde G., Bartsch H. Increased etheno-DNA adducts in affected tissues of patients suffering from Crohn's disease, ulcerative colitis, and chronic pancreatitis. Antioxid. Redox. Signal. 2006, 8:1003-1010.
8. Arab K., Pedersen M., Nair J., Meerang M., Knudsen L.E., Bartsch H. Typical signature of DNA damage in white blood cells: a pilot study on etheno adducts in Danish mother-newborn child pairs. Carcinogenesis 2009, 30:282-285.
9. Gyorffy E., Anna L., Gyori Z., Segesdi J., Minárovits J., Soltész I., Kostič S., Csekeo A., Poirier M.C., Schoket B. DNA adducts in tumour, normal peripheral lung and bronchus, and peripheral blood lymphocytes from smoking and non-smoking lung cancer patients: correlations between tissues and detection by 32P-postlabelling and immunoassay. Carcinogenesis 2004, 25:1201-1209.
10. Schoket B., Horkay I., Kósa Á., Páldeák L., Hewer A., Grover P.L., Phillips D.H. Formation of DNA adducts in the skin of psoriasis patients, in human skin in organ culture and in mouse skin and lung following topical application of coal-tar and juniper tar. J. Invest. Dermatol. 1999, 94:241-246.
11. Arif J.M., Dresler C., Clapper M.L., Gairola C.G., Srinivasan C., Lubet R.A., Gupta R.C. Lung DNA adducts detected in human smokers are unrelated to typical polyaromatic carcinogens. Chem. Res. Toxicol. 2006, 19:295-299.
12. Lloyd D.R., Phillips D.H., Carmichael P.L. Generation of putative intrastrand cross-links and strand breaks in DNA by transition metal ion-mediated oxygen radical attack. Chem. Res. Toxicol. 1997, 10:393-400.
13. Miller S.A., Dykes D.D., Polesky H.F. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res. 1988, 16:1215.
14. Kwasniewska A., Goździcka-Józefiak A., Borzecki A., Baranowski W. DNA adducts in squamous cell cervical carcinomas associated with HPV infection. Eur. J. Gynaecol. Oncol. 2004, 25:359-361.
15. Simons A.M., Phillips D.H., Coleman D.V. Damage to DNA in cervical epithelium related to smoking tobacco. BMJ 1993, 306:1444-1448.
16. Phillips D.H., Shé M.N. DNA adducts in cervical tissue of smokers and non smokers. Mutat. Res. 1994, 313:277-284.
17. Phillips D.H., Arlt V.M. The 32P-postlabeling assay for DNA adducts. Nat. Protoc. 2007, 2:2772-2781.
18. Reddy M.V., Randerath K. Nuclease P1-mediated enhancement of sensitivity of 32P-postlabeling test for structurally diverse DNA adducts. Carcinogenesis 1986, 7:1543-1551.
19. Szyfter K., Hemminki K. Analysis of deoxyribonucleic acid adducts in workers. Scand. J. Work Environ. Health 1992, 18:22-26.
20. Peluso M., Munnia A., Hoek G., Krzyzanowski M., Veglia F., Airoldi L., Autrup H., Dunning A., Garte S., Hainaut P., Malaveille C., Gormally E., Matullo G., Overvad K., Raaschou-Nielsen O., Clavel-Chapelon F., Linseisen J., Boeing H., Trichopoulou A., Trichopoulos D., Kaladidi A., Palli D., Krogh V., Tumino R., Panico S., Bueno-De-Mesquita H.B., Peeters P.H., Kumle M., Gonzalez C.A., Martinez C., Dorronsoro M., Barricarte A., Navarro C., Quiros J.R., Berglund G., Janzon L., Jarvholm B., Day N.E., Key T.J., Saracci R., Kaaks R., Riboli E., Vineis P. DNA adducts and lung cancer risk: a prospective study. Cancer Res. 2005, 65:8042-8048.