Influences of DMA isolation and RNA contamination on carcinogen-DNA adduct analysis by32P-postlabeling

Environmental and Molecular Mutagenesis

Volumn 32, Issue 4, 1998, Pages 344-350

  Godschalk, R W L ^a   Maas, L M ^a   Kleinjans, J C S ^a   Van Schooten, F J ^a  

^a MAASTRICHT UNIVERSITY   (Netherlands)

Author keywords

32P postlabelinq; Carcinoaen DNA adduct; DNA isolation; RNA contamination

Indexed keywords

BENZO[A]PYRENE; CARCINOGEN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTAMINATION; DNA ADDUCT; DNA DETERMINATION; HUMAN; HUMAN CELL; LIVER CANCER; LIVER CARCINOGENESIS; LYMPHOCYTE; MALE; NONHUMAN; RAT; RNA ANALYSIS;

7,8-DIHYDRO-7,8-DIHYDROXYBENZO(A)PYRENE 9,10-OXIDE; ANIMALS; DNA; DNA ADDUCTS; HUMANS; LIVER; LYMPHOCYTES; MALE; PHOSPHORUS RADIOISOTOPES; RATS; RATS, INBRED LEW; RNA;

EID: 0032417889     PISSN: 08936692     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1098-2280(1998)32:4<344::aid-em8>3.0.co;2-p     Document Type: Article

References (26)

1. Alexandrov K, Rojas M, Geneste O, Castegnaro M, Camus A-M, Petruzelli S, Giuntini C, Bartsch H. 1992. An improved fluorometric assay for dosimetry of benzo[a]pyrene diol epoxide DNA adducts in smokers' lung: Comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity. Cancer Res 52:6248-6253.
2. Allen JA, Coombs MM. 1980. Covalent binding of polycyclic aromatic compounds to mitochondria! and nuclear DNA. Nature 287:244-245.
3. Backer JM, Weinstein IB. 1980. Mitochondria! DNA is a major target for a dihydrodiol-epoxide derivative of benzo[a]pyrene. Science 209: 297-299.
4. Balansky R, Izotti A, Scatolini L, D'Agostini F, De Flora S. 1996. Induction and chemoprevention of adducts to mitochondria! DNA. Cancer Res 56:1642-1647.
5. Bøyum A. 1976. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol 5:9-15.
6. Brescia G, Foa V, Viezzer C, Celotti L, Assennato G. 1997. Aromatic DNA adducts in human peripheral blood lymphocytes and total white blood cells by 32P-postlabeling: Need for validation. Biomarkers 2:333-339.
7. Chomczynski P, Sacchi N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 161:156-159.
8. Dickey C, Santella RM, Haitis D, Tang D, Hsu Y, Cooper T, Young T-L, Perera FP. 1997. Variability in PAH-DNA adduct measurements in peripheral mononuclear cells: Implications for quantitative cancer risk assessment. Risk Anal 17:649-656.
9. Gallagher JE, Jackson MA, George MH, Lewtas J, Robertson IGC. 1989. Differences in detection of DNA adducts in the 32P-postlabeling assay after either 1-butano! extraction or nuclease PI treatment. Cancer Lett 45:7-12.
10. Gupta RC. 1984. Nonrandom binding of carcinogen N-hydroxy-2-acetylaminofluorene to repetitive sequences of rat liver DNA in vivo. Proc Natl Acad Sci USA 81:6934-6947.
11. Gupta RC, Reddy MV, Randerath K. 1982. 32P-Postlabeling analysis of nonradioactive aromatic carcinogen DNA adducts. Carcinogenesis 3:1081-1092.
12. Hemminki K, Grzybowska E, Chorazy M, Twardowska-Saucha K, Sroczynski JW, Putman KL, Randerath K, Phillips DH, Hewer A, Santella RM, Young TL, Perera FP. 1990. DNA adducts in humans environmentally exposed to aromatic compounds in an industrial area of Poland. Carcinogenesis 11:1229-1231.
13. Lewtas J, Walsh D, Williams R, Dobiàs L. 1997. Air pollution exposureDNA adduct dosimetry in humans and rodents: Evidence for nonlinearity at high doses. Mutât Res 378:51-64.
14. 32P-postlabeling assay. Carcinogenesis 10:1557-1559.
15. Miller SA, Dykes DD, Polesky HF. I988. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acid Res 16:1215.
16. 32P-postlabeling. In: Phillips DH, Castegnaro M, Bartsch H, eds. Postlabeling Methods for Detection of DNA Adducts. Lyon: International Agency for Research on Cancer (IARC) Scientific Publications, No. 124, pp 35-49.
17. 32P-postlabeling approaches for the detection of 8-oxo-2′-deoxyguanosine-3′-monophosphate in DNA. Mutât Res 378:139-149.
18. Randerath K, Randerath E. 1993. Postlabeling methods-an historical review. In: Phillips DH, Castegnaro M, Bartsch H, eds. Postlabeling Methods for Detection of DNA Adducts. Lyon: International Agency for Research on Cancer (IARC) Scientific Publications, No. 124, pp 3-9.
19. 32P-postIabeling test for structurally diverse DNA adducts. Carcinogenesis 7:1543-1551.
20. 32P-postlabeling test for covalent DNA binding of chemicals in vivo: Application to a variety of aromatic carcinogens and methylating agents. Carcinogenesis 5:231-243.
21. Savela K, Hemminki K, Hewer A, Phillips DH, Putman KL, Randerath K. 1989. Interlaboratory comparison of the 32P-postIabeling assay for aromatic DNA adducts in white blood cells of iron foundry workers. Mutât Res 224:485-492.
22. 32P-postlabeling procedure to quantify bulky aromatic DNA adducts. In: Phillips DH, Castegnaro M, Bartsch H, eds. Postlabeling Methods for Detection of DNA Adducts. Lyon: International Agency for Research on Cancer (IARC) Scientific Publications, No. 124, pp 65-70.
23. 32P-postIabeIing of aromatic DNA adducts in white blood cells and alveolar macrophages of smokers: Saturation at high exposures. Mutât Res 378:65-75.
24. 32P-postlabeIing assay for apurinic sites in DNA. Biochemistry 29:1737-1743.
25. Weyand EH, Bevan DR. 1987. Covalent binding of benzo[a]pyrene to macromolecules in lung and liver of rats following intratracheal instillation. Cancer Lett 36:149-159.
26. Zeisig M, Möller L. 1995.32P-HPLC suitable for characterisation of DNA adducts formed in vitro by polycyclic aromatic hydrocarbons and derivatives. Carcinogenesis 16:1-9.