Separation of supercoiled from open circular forms of plasmid DNA, and biological activity detection

Cytotechnology

Volumn 63, Issue 1, 2011, Pages 7-12

  Li, Huangjin ^a   Bo, Huaben ^a   Wang, Jinquan ^a   Shao, Hongwei ^a   Huang, Shulin ^a  

^a GUANGDONG PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Anion exchange chromatography; Gene therapy; Open circular plasmid DNA; Supercoiled plasmid DNA

Indexed keywords

ENDOTOXIN; GENOMIC DNA; GENOMIC RNA; PLASMID DNA;

ARTICLE; BIOLOGICAL ACTIVITY; COST EFFECTIVENESS ANALYSIS; DNA IMMUNIZATION; DNA PURIFICATION; DNA SUPERCOILING; ESCHERICHIA COLI; GENE THERAPY; GENETIC TRANSFECTION; NONHUMAN;

ESCHERICHIA COLI;

EID: 79951672127     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-010-9322-9     Document Type: Article

References (17)

1. M Bencina A Podgornik A Strancar 2004 Characterization of methacrylate monoliths for purification of DNA molecules J Sep Sci 27 801 810 1:CAS:528:DC%2BD2cXmtFKgsLc%3D 10.1002/jssc.200401784 (Pubitemid 39136466)
2. L Cupillard V Juillard S Latour G Colombet N Cachet S Richard, et al. 2005 Impact of plasmid supercoiling on the efficacy of a rabies DNA vaccine to protect cats Vaccine 23 1910 1916 1:CAS:528:DC%2BD2MXhslejur4%3D 10.1016/j.vaccine.2004.10.018 (Pubitemid 40289061)
3. MM Diogo JA Queiroz GA Monteiro SA Martins GN Ferreira DMF Prazeres 2000 Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography Biotechnol Bioeng 68 576 583 1:CAS:528:DC%2BD3cXjt1GrtbY%3D 10.1002/(SICI)1097-0290(20000605)68:5<576:: AID-BIT13>3.0.CO;2-5 (Pubitemid 30307729)
4. MM Diogo SC Ribeiro JA Queiroz GA Monteiro N Tordo P Perrin, et al. 2001 Production, purification and analysis of an experimental DNA vaccine against rabies J Gene Med 3 577 584 1:STN:280:DC%2BD38%2Fltlaqtw%3D%3D 10.1002/jgm.218 (Pubitemid 33685088)
5. MM Diogo JA Queiroz DMF Prazers 2003 Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography J Chromatogr A 998 109 117 1:CAS:528:DC%2BD3sXkt1yisb8%3D 10.1016/S0021-9673(03)00618-6 (Pubitemid 36629465)
6. MM Diogo JA Queiroz DMF Prazeres 2005 Chromatography of plasmid DNA J Chromatogr A 1069 3 22 1:CAS:528:DC%2BD2MXitlOht78%3D 10.1016/j.chroma.2004.09. 050 (Pubitemid 40385093)
7. GNM Ferreira JMS Cabral DMF Prazeres 1997 A comparison of gel filtration chromatographic supports for plasmid purification Biotechnol Tech 11 417 420 1:CAS:528:DyaK2sXktF2nur0%3D 10.1023/A:1018420807203 (Pubitemid 27253594)
8. S Gurunathan DM Klinman RA Seder 2000 DNA vaccines: immunology, application, and optimization Ann Rev Immunol 18 927 974 1:CAS:528: DC%2BD3cXjs1Gmt7w%3D 10.1146/annurev.immunol.18.1.927 (Pubitemid 30365401)
9. RN Hines KC O'Connor G Vella W Warren 1992 Large-scale purification of plasmid DNA by anion-exchange high-performance liquid chromatography Biotechniques 12 430 434 1:CAS:528:DyaK38XktVWru7k%3D
10. NA Horn JA Meek G Budahazi M Marquet 1995 Cancer gene therapy using plasmid DNA: purification of DNA for human clinical trials Hum Gene Ther 6 565 573 1:CAS:528:DyaK2MXmsVGkt7k%3D 10.1089/hum.1995.6.5-565
11. S Iuliano JR Fisher M Chen WJ Kelly 2002 Rapid analysis of a plasmid by hydrophonic-interaction chromatography with a non-porous resin J Chromatogr A 972 77 86 1:CAS:528:DC%2BD38XmvVChu70%3D 10.1016/S0021-9673(02)00901-9 (Pubitemid 35246330)
12. VB Pillai M Hellerstein T Yu RR Amara HL Robinson 2008 Comparative studies on in vitro expression and in vivo immunogenicity of supercoiled and open circular forms of plasmid DNA vaccines Vaccine 26 1136 1141 1:CAS:528:DC%2BD1cXhslelu7o%3D 10.1016/j.vaccine.2007.10.023
13. DMF Prazeres T Schluep C Cooney 1998 Preparative purification of supercoiled plasmid DNA using anion-exchange chromatography J Chromatogr A 806 31 45 1:CAS:528:DyaK1cXjsFGltb0%3D 10.1016/S0021-9673(97)01254-5 (Pubitemid 28238201)
14. LM Sandberg A Bjurling P Busson J Vasi R Lemmens 2004 Thiophilic interaction chromatography for supercoiled plasmid DNA purification J Biotech 109 193 199 1:CAS:528:DC%2BD2cXivVKmsbw%3D 10.1016/j.jbiotec.2003.10.036 (Pubitemid 38452584)
15. M Schleef T Schmidt 2004 Animal-free production of ccc-supercoiled plasmids for research and clinical applications J Gene Med 6 S45 S53 1:CAS:528:DC%2BD2cXislOms70%3D 10.1002/jgm.511 (Pubitemid 40228016)
16. T Schluep CL Cooney 1998 Purification of plasmids by triplex affinity interaction Nucleic Acids Res 26 4524 4528 1:STN:280:DyaK1cvhs1aitg%3D%3D 10.1093/nar/26.19.4524 (Pubitemid 28442148)
17. P Wils V Escriou A Warnery F Lacroix D Lagneaux M Ollivier, et al. 1997 Efficient purification of plasmid DNA for gene transfer using triple-helix affinity chromatography Gene Ther 4 323 330 1:CAS:528:DyaK2sXisVyktrg%3D 10.1038/sj.gt.3300388 (Pubitemid 27170725)