Characterization of native and denatured ricin using MALDI-TOF/MS

Cellular and Molecular Biology

Volumn 56, Issue SUPPL.1, 2010, Pages

  Sehgal, P ^a   Rao, M K ^a   Kumar, O ^a   Vijayaraghavan, R ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

Author keywords

Ambiguity; Denatured; Immunocapture; MALDI TOF MS; Marker; Peptide mass fingerprinting; Ricin

Indexed keywords

RICIN; RICIN A; PROTEIN SUBUNIT;

APPLE JUICE; ARTICLE; CONTROLLED STUDY; DEGLYCOSYLATION; DENATURATION; GEL FILTRATION CHROMATOGRAPHY; HEAT TREATMENT; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MILK; MOLECULAR WEIGHT; NONHUMAN; PANIC; POLYACRYLAMIDE GEL ELECTROPHORESIS; WESTERN BLOTTING; AMINO ACID SEQUENCE; CHEMISTRY; FOOD CONTAMINATION; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; PROTEIN DENATURATION; TWO DIMENSIONAL GEL ELECTROPHORESIS;

MALUS X DOMESTICA; RICINUS COMMUNIS;

AMINO ACID SEQUENCE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FOOD CONTAMINATION; MOLECULAR SEQUENCE DATA; PROTEIN DENATURATION; PROTEIN SUBUNITS; RICIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 79951575686     PISSN: 01455680     EISSN: 1165158X     Source Type: Journal    
DOI: 10.1170/158     Document Type: Article

References (39)

1. Cawley, D.B. and Houston, L.L., Effect of sulhydryl reagents and protease inhibitors on sodium dodecyl sulphate-heat induced dissociation of Ricinus communis agglutinin. Biochim. Biophys. Acta 1979, 81: 51-62.
2. Chaponi, L. and Migliorini, P., Antibody usage in the lab: immunoblotting. Springer-Verlag Press, New York, 2005, pp. 32-46.
3. Darby, S.M., Miller, M.L. and Allen, R.O., Forensic determination of ricin and alkaloid marker ricinine from castor bean extracts. J. Forensic Sci. 2001, 46:1033-1042.
4. Despeyroux, D., Walker, N., Pearce, M., Fisher, M., McDonnell, M., Bailey, S.C., Griffiths, G.D., Watts, P., Characterization of ricin heterogeneity by electrospray mass spectrometry, capillary electrophoresis, and resonant mirror. Anal. Biochem. 2000, 279: 23-36.
5. Duriez, E., Fenialle, F., Tabet, J.C., Lamourette, P., Hilarie, D., Becher, F., Ezan, E., Detection of ricin in complex samples by immunocapture and matrix-assisted laser desorption/ionization of time-of-flight mass spectrometry. J. Proteome. Res. 2008, 7: 4154-4163.
6. Emmaneul, F., Turpin, E., Alfsen, A., Frenoy, J. P., Separation of A-and B- Chains after Dithiothreitol reduction. Anal. Biochem. 1988, 173: 134-141.
7. Endo, Y. and Tsurugi, K., RNA glycosidase activity of ricin A-chain. J. Biol Chem. 1987, 262: 8128-8130.
8. Fredriksson, S.A., Hulst, A.G., Artursson, E., de Jong, A.L., Nilsson, C., van Barr, B.L., Forensic identification of neat ricin and of ricin from crude castor bean extracts by mass spectrometry. Anal. Chem. 2005, 77: 1545-1555.
9. Griffiths, G.D., Lindsay, C.D., Allenby, A.C., Bailey, S.C., Scawin, J.W., Ric,e P., Upshall, D.G., Protection against inhalation toxicity of ricin and abrin by Immunization. Human Exp Toxicol. 1995, 14: 155-164.
10. Haberling, R.L., Kalter, S.S., Smith, J.S., Hilderbrad, D.G., Serodiagnosis of rabies by DOT immunobinding assay. J. Immunol. Methods 1987, 37: 909-922.
11. Hames, B.D., One dimensional polyacrylamide gel electrophoresis of proteins. In: Gel electrophoresis of protein: a practical approach, Hames, B.D. (ed.), Oxford University press, New York, 1990, pp. 1-147.
12. He, X., Carter, J.M., Brandon, C.D., Cheng, L.W., Mickeon, T.A., Application of a real time polymerase chain reaction method to detect castor toxin contamination in fluid milk and eggs. J. Agric. Food Chem. 2007, 55: 6897-6902.
13. He, X., Lu, S., Cheng, L.W., Rasooly, R., Carter, J.M., Effect of food matrices on the biological activity of ricin. J. Food Prot. 2008, 71: 2053-2058.
14. Hegde, R., Maiti, T.K. and Podder, S.K., Purification and characterization of three toxins and two agglutinins from Abrus precatorious seed by using lactamyl-Sepharose chromatography. Anal. Biochem. 1991, 194: 101-109.
15. Jenkins, F.P., Allergenic and toxic componentsof castor bean meal: Review of the literature and studies of the inactivation of these components. J. Sci. Food. Agric. 1963, 14: 773-780.
16. Kalb, S.R. and Barr, J.R., Mass Spectrometric detection of ricin and its activity in food and clinical samples. Anal. Chem. 2009, 81: 2037-2042.
17. Kaufmann, R., Matrix-assisted laser desorption ionization (MALDI) mass spectrometry. A novel analytical tool in mol.biology and biotechnology. J. Biotechnol. 1995, 41: 155-175.
18. Kimura, Y., Hase, S., Kobayashi, Y., Kyogoku, Y., Structures of sugar chains of ricin D. J. Biochem. 1988, 103: 944-949.
19. Kimura, Y., Kusuoku, H., Tada, M., Takagi, S., Funatsu, G., Structural analyses of sugar chains from Ricin-A chain. Agric. Biol. Chem.1990, 54: 157-162.
20. Kumar, O., Pradhan, S., Sehgal, P., Singh, Y., Rajagopalan, V., Denatured ricin can be detected as native ricin by immunological methods, but non-toxic invivo. J. Forensic Sci. 2009, 55: 801-807.
21. Kumar, O., Sugendran, K., Vijayaraghavan, R., Oxidative stress associated hepatic and renal toxicity induced by ricin in mice. Toxicon 2003, 41: 333-338.
22. Laemmli, U.K., Clevage of structure proteins during the assembly of the head of the bacteriophage T4. Nature 1970, 227: 680-687.
23. Levy, M. and Benaglia, A.E., The influence of temperature and pH upon the rate of denaturation of ricin. J. Biol. Chem. 1950, 186: 829-847.
24. Luginer, A. and Dirheimer, G., Differences between ricin and phytohemagglutinins from Ricinus communis seeds. FEBS Letter 1973, 35: 117-120.
25. Muse, T., Funatsu, G. and Funatsu, M., Isolation and characterization of ricin E from castor seeds. Agric. Biol. Che. 1977, 41: 2041-2046.
26. Na, D.H., Cho, C.K., Youn, Y.S., Choi, Y., Lee, K.R., Yoo, S.D., Lee, K.C., Capillary electrophoresis to characterize ricin and its subunits with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Toxicon 2004, 43: 329-335.
27. Nicolson, G.L., Blaustein, J. and Etzler, M.E., Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma. Biochemistry 1974, 13: 197-204.
28. O'Farrel, P.H., High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 1975, 250: 4007- 4021.
29. Okorie, A.U. and Anugwa F.O.I., The feeding value of roasted castor bean (Ricinus communis) to growing cheeks. Plant Foods Human Nutr. 1987, 37: 97-102.
30. Olsnes, S., The history of ricin, abrin and related toxins. Toxicon 2004, 44: 361-370
31. Olsnes, S. and Phil, A., Different biological properties of two constituent peptide chain of ricin, a toxic protein inhibiting protein synthesis. Biochemistry 1973, 12: 3132-3136.
32. Ostin, A., Bergstrom, T., Fredriksson, S.A., Nilsson, C., Solvent assisted trypsin digestion of ricin for forensic identification by LC-ESI MS/MS. Anal. Chem. 2007, 79: 6271-6278
33. Robertus, J.D., Yan, X., Ernst, S., Monzingo, A., Worley, S., Day, P., Hollis, T., Svinth, M., Structural analysis of ricin and implications for inhibitor design. Toxicon 1996, 34: 1325-1334.
34. Shyu, R.H., Chiano, D.J., Liu, H.W., Tang, S.S., Monoclonal antibody-based enzyme immunoassay for detection of ricin. Hybrid Hybridomics 2002, 21: 69-73.
35. Tregar, J.W. and Roberts, L.M., The lectin gene family of Ricinus communis: cloning of a functional ricin gene and three lectin pseudogenes. Plant Mol. Biology 1992, 18: 515-525.
36. Volkin, D.B. and Kilbanov, A.M., Thermal destruction processes in proteins involving cystine residues. J. Biol. Chem. 1987, 262: 2945-2950.
37. Werner, W.E., Demorest, D.M., Stevens, J., Wiktorowicz, J.E., Size-dependent separation of proteins denatured in SDS by capillary electrophoresis using a replacable sieving matrix. Anal. Biochem. 1993, 212: 253- 258.
38. Woo, B.H., Lee, J.T., Na, D.H., Lee, K.C., Sepharose-unbinding ricin E as a source of ricin A chain immunotoxin. J. Immunol. Methods 2001, 249: 91-98.
39. Woo, B.H. and Lee, K.C., Purification of sepharose unbinding ricin from castor beans by hydroxyapatite chromatography. Protein Expression Purif. 1998, 13:154-164