Denatured ricin can be detected as native ricin by immunological methods, but nontoxic in vivo

Journal of Forensic Sciences

Volumn 55, Issue 3, 2010, Pages 801-807

  Kumar, Om ^a   Pradhan, Santwana ^a   Sehgal, Payal ^a   Singh, Yamini ^a   Vijayaraghavan, Rajagopalan ^a  

^a DEFENCE RESEARCH AND DEVELOPMENT ESTABLISHMENT   (India)

Author keywords

Denatured ricin; Dot ELISA; Forensic sciences; ICT; Ricin; SDS PAGE; Toxicity

Indexed keywords

RICIN;

ANIMAL; ARTICLE; CHEMISTRY; FORENSIC TOXICOLOGY; IMMUNOASSAY; LATEX AGGLUTINATION TEST; METHODOLOGY; MOUSE; PROTEIN DENATURATION; PROCEDURES; TOXICITY;

ANIMALS; FORENSIC TOXICOLOGY; IMMUNOASSAY; LATEX FIXATION TESTS; MICE; PROTEIN DENATURATION; RICIN;

EID: 77951130738     PISSN: 00221198     EISSN: 15564029     Source Type: Journal    
DOI: 10.1111/j.1556-4029.2009.01290.x     Document Type: Article

References (29)

1. Pinkerton SD, Rolfe R, Auld DL, Ghetie V, Lauterbach BF. Selection of castor for divergent concentrations of ricin and Ricinus communis agglutinin. Crop Sci 2007 39 : 353 357.
2. Olsnes S, Pihl A. Different biological properties of two constituent peptide chains of ricin, a toxic protein inhibiting protein synthesis. Biochemistry 1973 12 : 3121 3126.
3. Endo Y, Mitsui K, Motizuki M, Tsurugi K. The mechanism of action of ricin and related toxic lectins on eukaryotic ribosomes. The site and the characteristics of the modification in 28 S ribosomal RNA caused by the toxins. J Biol Chem 1987 262 : 5908 5912.
4. Endo Y, Tsurugi K. The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA. J Biol Chem 1988 263 : 8735 8739.
5. Olsnes S. The history of ricin, abrin and related toxins. Toxicon 2004 44 : 361 370.
6. Vittetta ES, Thorpe PE. Immunotoxin containing ricin or its A chain. Semin Cell Biol 1991 2 : 47 58.
7. Knight B. Ricin - a potent homicidal poison. Br Med J 1979 3 : 350 351.
8. Crompton R, Gall D. Georgi Markov - death in a pellet. Med Leg J 1980 48 : 48 62.
9. Olsnes S. Ricin, the deadly poison of castor beans. Biotechnology in Warfare February/March 2003 20 1.
10. Griffith GD, Newman H, Gee DJ. Identification and quantification of ricin toxin in animal tissues using ELISA. J Forensic Sci Soc 1986 26 : 349 358.
11. Shyu RH, Chiao DJ, Liu HW, Tang SS. Monoclonal antibody based enzyme immunoassay for detection of ricin. Hybrid Hybridomics 2002 21 : 69 73.
12. Godal A, Olsnes S, Pihl A. Radioimmunoassay of abrin and ricin in blood. J Toxicol Environ Health 1981 8 : 409 417.
13. Franz DR, Jaax NK. Ricin toxin. In : Zajtchuk R, Bellamy RF, editors. Textbook of military medicines.
14. Shyu RH, Shyu HF, Liu HW, Tang SS. Colloidal gold based immunochromatographic assay for detection of ricin. Toxicon 2002 40 : 255 258.
15. Narang U, Anderson GP, Lifler FS, Burans J. Fiber optic based biosensor for ricin. Biosens Bioelectron 1997 12 : 937 945.
16. Rubina AY, Dyukova VI, Dementieva EI, Stomakhin AA, Nesmeyanov VA, Grishin EV, et al. Quantitative immunoassay of biotoxins on hydrogel based protein microchips. Anal Biochem 2005 340 : 317 329.
17. Lubelli C, Chatgilialoglu A, Bolognesi A, Strocchi P, Colombatti M, Stirpe F. Detection of ricin and other ribosome-inactivating proteins by an immunopolymerase chain reaction assay. Anal Biochem 2006 355 : 102 109.
18. Kumar O, Sugendran K, Vijayaraghavan R. Oxidative stress associated hepatic and renal toxicity induced by ricin in mice. Toxicon 2003 41 : 333 338.
19. Hegde R, Maiti TK, Poddar SK. Purification and characterization of three toxins and two agglutinins from Abrus precatorius seed by using lactamyl-sepharose affinity chromatography. Anal Biochem 1991 194 : 101 109.
20. Kumar O, Rai GP, Parida MM, Vijayaraghavan R. Rapid detection of ricin by sensitizing carboxylated latex particles by ricin antibodies. Def Sci J 2004 54 : 57 63.
21. Inzana TJ. Simplified procedure for preparation of sensitized latex particles to detect capsular polysaccharides: application to typing and diagnosis of Actinobacillus pleuropneumoniae. J Clin Microbiol 1995 33 : 2297 2303.
22. Laemmli UK. Cleavage of structure proteins during the assembly of the head of the bacteriophage T4. Nature 1970 227 : 680 687.
23. Chaponi L, Migliorini P, editors. Antibody usage in the lab: immunoblotting.
24. Hames BD. One-dimensional polyacrylamide gel electrophoresis. In : Hames BD, Rickwood D, editors. Gel electrophoresis of proteins: a practical approach.
25. CDC Report. Investigation of a ricin-containing envelope at a postal facility - South Carolina. MMWR Morb Mortal Wkly Rep 2003 52 (46 1129 1131.
26. Poli MA, Rivera VR, Hewetson JF, Merill GA. Detection of ricin by colorimetric and chemiluminescence ELISA. Toxicon 1994 32 : 1371 1377.
27. Cook DL, Jonathan D, Griffiths GD. Retrospective identification of ricin in animal tissues following administration by pulmonary and oral routes. Toxicology 2006 223 : 61 70.
28. Malcolm A, Brian P. A sensitive immunosorbent assay for the detection of ricin in blood, Defence Science and Technology Organisation (DSTO), Report No. DSTO-TR-0572, 1997.
29. Lugnier A, Dirheimer G. Differences between ricin and phytohemagglutinins from Ricinus communis seeds. FEBS Lett 1973 35 : 117 120.