Analysis of interspecific variation in relative fatty acid composition: Use of flow cytometry to estimate unsaturation index and relative polyunsaturated fatty acid content in microalgae

Journal of Applied Phycology

Volumn 23, Issue 1, 2011, Pages 7-15

  Guzmán, Héctor Mendoza ^a   de la Valido, Adelina Jara ^a   Duarte, Laura Carmona ^a   Presmanes, Karen Freijanes ^a  

^a Instituto Tecnologico de Canarias   (Spain)

Author keywords

Fatty acids; Flow cytometry; Lipids; Microalgae; Nile Red

Indexed keywords

ANIMALIA;

EID: 79951551205     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10811-010-9526-6     Document Type: Article

References (32)

1. Alonzo F, Mayzaud P (1999) Spectrofluorometric quantification of neutral and polar lipids in zooplankton using Nile Red. Mar Chem 67: 289-301.
2. Andreu V, Collados R, Testillano PS, Risueño MC, Picorel R, Alfonso M (2007) In situ molecular identification of the pastid ω3 fatty acid desaturase FAD7 from soybean: evidence of thylakoid membrane localization. Plant Physiol 145: 1336-1344.
3. Bai J, Pegano RE (1997) Measurement of spontaneous transfer and transbilayer movement of BODIPY-labeled lipids in lipids vesicles. Biochemistry 36(29): 8840-8848.
4. Chen W, Chengwu Z, Song L, Sommerfeld M, Hu Q (2009) A high throughput Nile red method for quantitative measurement of neutral lipids in microalgae. J Microbiol Meth 77: 41-47.
5. Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25: 294-306.
6. Cooksey KE, Guckert JB, Williams GA, Callis PR (1987) Fluorometric determination of the neutral lipid content of microalgal cells using Nile Red. J Microbiol Methods 6: 333-346.
7. Da Silva TL, Santos CA, Reis A (2009) Multi-parameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. Biotechnol Bioprocess Eng 14(3): 330-337.
8. Davey HM, Kell DB (1996) Flow cytometry and cell sorting of heterogeneous microbial populations-the importance of single-cell analyses. Microbioll Rev 60: 641-696.
9. de la Jara A, Mendoza H, Martel A, Molina C, Nordströn C, de la Rosa V, Díaz R (2003) Flow cytometric determination of lipid content in marine dinoflagellate Crypthecodinium cohnii. J Appl Phycol 15: 433-438.
10. Ferrari RA, de Souza WL (2009) Evaluation of oxidation stability of sunflower oil biodiesel with antioxidants. Quim Nova 32(1): 106-111.
11. Gouveia L, Marques AE, da Silva TL, Reis A (2009) Neochloris oleoabundas UTX#1185: a suitable renewable lipid source for biodiesel production. J Ind Microbiol Biotechnol 36: 821-826.
12. Griffith MJ, Harrison TL (2009) Lipid productivity as a key characteristic for choosing algal species for biodiesel production. J Appl Phycol 21: 493-507.
13. Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanle MH (eds) Culture invertebrate animals. Plenum, New York, pp 26-60.
14. Guimarães ARP, Costa Rosa LFBP, Sitnik RH, Curi R (1991) Effect of polyunsaturates (PUFA n-6) and saturated fatty acid-rich diets on macrophage metabolism and function. Biochem Int 23: 1739-1751.
15. Hu Q, Sommerfeld M, Jarvis E, Ghirardi E, Posewitz M, Seibert M, Darzins A (2008) Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances. Plant J 54: 621-639.
16. Juaneda P, Rocquelin G (1985) Rapid and convenient separation of phospholipids in rat heart using silica cartridges. Lipids 20: 40-41.
17. Li Q, Du W, Liu D (2008) Perspective of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80: 749-756.
18. Mendoza H, Martel A, Jiménez del Río M, García-Reina G (1999) Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina. J Appl Phycol 11: 15-19.
19. Mendoza H, de la Jara A, Carmona Duarte L, Freijanes Presmanes K (2009) Estimate by means of flow cytometry of variation in composition of fatty acids from Tetraselmis suecica in response to culture conditions. Aquac Int. doi: 10. 1007/s10499-008-9235-1.
20. Muller-Feuga A (2000) The role of microalgae in aquaculture: situation and trends. J Appl Phycol 12: 527-534.
21. Mustardy L, Los DA, Gombos Z, Murata N (1996) Immunocytochemical localization of acyl-lipid desaturases in cyanobacterial cells: evidence that both thylakoid membranes and cytoplasmic membranes are sites of lipid desaturation. Proc Natl Acad Sci USA 93: 10524-10527.
22. Patil V, Källqvist T, Olsen E, Vogt G, Gislerød HR (2007) Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquac Int 15: 1-9.
23. Pulz O, Cross W (2004) Valuable products from biotechnology of microalgae. J Appl Microbiol Biotechnol 65: 635-648.
24. Rao IM, Terry N (2000) Photosynthetic adaptation to nutrient stress. In: Yunus M, Pathre U, Mohanty P (eds) Probing photosynthesis mechanisms, regulation and adaptation. New York, Taylor & Francis, pp 380-397.
25. Roessler PG (1990) Environmental control of glycerolipids metabolism in microalgae: commercial implications and future research directions. J Phycol 26: 393-399.
26. Semenenko VE, Abdullaev AA (1980) Parametric control of β-carotene biosynthesis in Dunaliella salina cells under conditions of intensive cultivation. Sov Plant Physiol 27: 22-30.
27. Thi P, Borrel-Flood C, Vieira da Silva J, Justin AM, Mazliak P (1987) Effects on lipid metabolism in cotton leaves. Phytochemistry 24: 723-727.
28. Ward OP, Singh A (2005) Omega-3/6 fatty acids: alternative sources of productions. Proc Biochem 40: 3267-3652.
29. Wikfors GH, Ohno M (2001) Impact of algal research in aquaculture. J Phycol 37: 968-974.
30. Wu Q, Liu T, Liu H, Zheng GC (2009) Unsaturated fatty acid: metabolism, synthesis and gene regulation. Afr J Biotechnol 8: 1782-1785.
31. Yokthongwattana K, Savchenko T, Polle JEW, Melis A (2005) Isolation and characterization of a xanthophylls-rich fraction from the thylakoid membrane of Dunaliella salina (green algae). Photochem Photobiol Sci 4: 1028-1034.
32. Yongmanitchai W, Ward OP (1992) Separation of lipid classes from Phaeodactylum tricornutum using silica cartridges. Phytochemistry 31: 3405-3408.