Efficient Agrobacterium-based transient expression system for the production of biopharmaceuticals in plants

Bioengineered Bugs

Volumn 1, Issue 3, 2010, Pages 221-224

  Circelli, Patrizia ^a   Donini, Marcello ^a   Villani, Maria Elena ^a   Benvenuto, Eugenio ^a   Marusic, Carla ^a  

^a ENEA C R CASACCIA   (Italy)

Author keywords

Agrobacterium tumefaciens; Agroinfiltration; Gene silencing suppressor; HIV; Immunoglobulin; Molecular farming

Indexed keywords

COAT PROTEIN; HUMAN IMMUNOGLOBULIN;

ARTICHOKE MOTTLED CRINCKLE VIRUS; ARTICLE; CONTROLLED STUDY; GENE EXPRESSION SYSTEM; GENE SILENCING; HEAVY CHAIN; LIGHT CHAIN; NONHUMAN; PLANT VIRUS; PLANT YIELD COMPONENT; RHIZOBIUM RADIOBACTER;

AGROBACTERIUM; BIOENGINEERING; GENE EXPRESSION; HUMANS; PHARMACEUTICAL PREPARATIONS; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TOBACCO;

AGROBACTERIUM; AGROBACTERIUM TUMEFACIENS; ARTICHOKE MOTTLED CRINKLE VIRUS; CYNARA SCOLYMUS; HUMAN IMMUNODEFICIENCY VIRUS 1; NICOTIANA BENTHAMIANA; POTATO VIRUS X; TOMATO BUSHY STUNT VIRUS;

EID: 79851482941     PISSN: 19491018     EISSN: 19491026     Source Type: Journal    
DOI: 10.4161/bbug.1.3.11722     Document Type: Article

References (15)

1. Spök A, Twyman RM, Fischer R, Ma JK, Sparrow PA. Evolution of a regulatory framework for pharmaceuticals derived from genetically modified plants. Trends Biotechnol 2008; 26:506-17.
2. Daniell H, Sheatfield SJ, Wycoff K. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 2001; 6:219-26.
3. Orzáez D, Granell A, Blázquez MA. Manufacturing antibodies in the plant cell. Biotechnol J 2009; 4:1712-24.
4. Marusic C, Vitale A, Pedrazzini E, Donini M, Frigerio L, Bock R, et al. Plant-based strategies aimed at expressing HIV antigens and neutralizing antibodies at high levels. Nef as a case study. Transgenic Res 2009; 18:499-512.
5. Simmons CW, VanderGheynst JS. Transient co-expression of post-transcriptional gene silencing suppressors and beta-glucuronidase in harvested lettuce leaf tissue does not improve recombinant protein accumulation in planta. Biotechnol Lett 2007; 29:641-5.
6. Sainsbury F, Lomonossoff GP. Extremely high-level and rapid transient protein production in plants without the use of viral replication. Plant Physiol 2008; 148:1212-8.
7. Villani ME, Morgun B, Brunetti P, Marusic C, Lombardi R, Pisoni I, et al. Plant pharming of a full-sized, tumour-targeting antibody using different expression strategies. Plant Biotech J 2009; 7:59-72.
8. Kathuria S, Sriraman R, Nath R, Sack M, Pal R, Artsaenko O, et al. Efficacy of plant-produced recombinant antibodies against HCG. Hum Reprod 2002; 17:2054-61.
9. Hull AK, Criscuolo CJ, Mett V, Groen H, Steeman W, Westra H, et al. Human-derived, plant-produced monoclonal antibody for the treatment of anthrax. Vaccine 2005; 18:2082-6.
10. Strasser R, Stadlmann J, Schähs M, Stiegler G, Quendler H, Mach L, et al. Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure. Plant Biotech J 2008; 6:392-402.
11. Johansen LK, Carrington JC. Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system. Plant Physiol 2001; 126:930-8.
12. Voinnet O, Rivas S, Mestre P, Baulcombe D. An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus. Plant J 2003; 33:949-56.
13. Lombardi R, Circelli P, Villani ME, Buriani G, Nardi L, Coppola V, et al. High-level HIV-1 Nef transient expression in Nicotiana benthamiana using the P19 gene silencing suppressor protein of Artichoke Mottled Crinckle Virus. BMC Biotechnology 2009; 9:96.
14. Marusic C, Nuttall J, Buriani G, Lico C, Lombardi R, Baschieri S, et al. Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells. BMC Biotechnol 2007; 26:12.
15. Tavladoraki P, Benvenuto E, Trinca S, De Martinis D, Cattaneo A, Galeffi P. Transgenic plants expressing a functional single-chain Fv antibody are specifically protected from virus attack. Nature 1993; 366:469-72.