Multiphoton microscopy of engineered dermal substitutes: Assessment of 3D collagen matrix remodeling induced by fibroblasts contraction

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 7548, Issue , 2010, Pages

  Pena, A M ^a   Olive, C ^a   Michelet, J F ^a   Galey, J B ^a   Fagot, D ^a   Leroy, F ^a   Martin, J L ^b   Colonna, A ^a   Schanne Klein, M C ^b  

^a L ORÉAL RECHERCHE   (France)

^b LABORATOIRE D OPTIQUE ET BIOSCIENCES   (France)

Author keywords

Collagen; dermal substitutes; fibroblasts; multiphoton microscopy; Second Harmonic Generation

Indexed keywords

ANTI-AGING; COLLAGEN FIBRILS; COLLAGEN MATRICES; CONTROL MEDIUMS; CONTROL SAMPLES; CULTURE MEDIUM; DERMAL FIBROBLASTS; DERMAL SUBSTITUTES; DERMAL TISSUES; EXTRACELLULAR MATRICES; IN-CONTROL; IN-SITU; INHIBITORY EFFECT; KINASE INHIBITORS; MATRIX; MECHANICAL FORCE; MULTIPHOTON MICROSCOPY; SECOND HARMONIC GENERATION;

CELL CULTURE; COLLAGEN; ENZYME INHIBITION; ENZYMES; FIBROBLASTS; HARMONIC GENERATION; NONLINEAR OPTICS; THREE DIMENSIONAL;

TISSUE;

EID: 79751491527     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.839947     Document Type: Conference Paper

References (13)

1. B. R. Masters, P. T. C. So, and E. Gratton, "Multiphoton excitation fluorescence microscopy and spectroscopy of in vivo human skin," Biophys. J., 72, 2405-2412 (1997).
2. W. R. Zipfel, R. M. Williams, R. Christie, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, "Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.," Proc. Natl. Acad. Sci. USA, 100, 7075-7080 (2003).
3. K. König, and I. Riemann, "High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution," J. Biomed. Opt., 8(3), 432-439 (2003).
4. A.-M. Pena, M. Strupler, T. Boulesteix, G. Godeau, and M.-C. Schanne-Klein, "Spectroscopic analysis of keratin endogenous signal for skin multiphoton microscopy," Opt. Express, 13(16), 6268-6274; erratum: 13(17)6667 (2005).
5. J. A. Palero, H. S. d. Bruijn, A. v. d. P.-v. d. Heuvel, H. J. C. M. Sterenborg, and H. C. Gerritsen, "In vivo nonlinear spectral imaging in mouse skin," Opt. Express, 14(10), 4395-4402 (2006).
6. A.-M. Pena, A. Fabre, D. Débarre, J. Marchal-Somme, B. Crestani, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, "Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy.," Microsc. Res. Tech., 70(2), 162-170 (2007).
7. M. Strupler, M. Hernest, C. Fligny, J.-L. Martin, P.-L. Tharaux, and M.-C. Schanne-Klein, "Second Harmonic Microscopy to Quantify Renal Interstitial Fibrosis and Arterial Remodeling," Journal of Biomedical Optics, 13, 054041 (2008).
8. M. Strupler, A.-M. Pena, M. Hernest, P.-L. Tharaux, J.-L. Martin, E. Beaurepaire, and M.-C. Schanne-Klein, "Second harmonic imaging and scoring of collagen in fibrotic tissues," Opt. Express, 15(7), 4054-4065 (2007).
9. E. Bell, B. Ivarsson, and C. Merrill, "Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro," Proc Natl Acad Sci U S A, 76(3), 1274-8 (1979).
10. E. Bell, S. Sher, B. Hull, C. Merrill, S. Rosen, A. Chamson, D. Asselineau, L. Dubertret, B. Coulomb, C. Lapiere, B. Nusgens, and Y. Neveux, "The Reconstitution of Living Skin," J Investig Dermatol, 81(s1), 2s-10s (1983).
11. C. Bayan, J. M. Levitt, E. Miller, D. Kaplan, and I. Georgakoudi, "Fully automated, quantitative, noninvasive assessment of collagen fiber content and organization in thick collagen gels," Journal of Applied Physics, 105, 102042 (2009).
12. C. B. Raub, V. Suresh, T. Krasieva, J. Lyubovitsky, J. D. Mih, A. J. Putnam, B. J. Tromberg, and S. C. George, "Noninvasive Assessment of Collagen Gel Microstructure and Mechanics Using Multiphoton Microscopy," Biophysical Journal, 92, 2212-2222 (2007 ).
13. M. Uehata, T. Ishizak, H. Satoh, T. Ono, T. Kawahara, Tamami Morishita, H. Tamakawa, K. Yamagami, J. Inui, M. Maekawa, and S. Narumiya, "Calcium sensitization of smooth muscle mediated by a Rho-associated protein kinase in hypertension," Nature, 389, 990 (1997).