A single mutation responsible for temperature-sensitive entry and assembly defects in the VP1-2 protein of herpes simplex virus

Journal of Virology

Volumn 85, Issue 5, 2011, Pages 2024-2036

  Abaitua, F ^a   Daikoku, T ^b   Crump, C M ^c   Bolstad, M ^a   O'Hare, P ^a  

^a IMPERIAL COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF TOYAMA   (Japan)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN VP1; PROTEIN VP2;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; DEFECTIVE VIRUS; HERPES SIMPLEX VIRUS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DEFECT; PROTEIN EXPRESSION; TEMPERATURE SENSITIVE MUTANT; TEMPERATURE SENSITIVITY; VIRUS ENTRY; VIRUS REPLICATION; VIRUS STRAIN; WILD TYPE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; HEP G2 CELLS; HERPES SIMPLEX; HERPESVIRUS 1, HUMAN; HUMANS; MOLECULAR SEQUENCE DATA; POINT MUTATION; SEQUENCE ALIGNMENT; TEMPERATURE; VIRAL PROTEINS; VIRUS ASSEMBLY; VIRUS INTERNALIZATION; VIRUS REPLICATION;

HUMAN HERPESVIRUS 1; MIRIDAE; SIMPLEXVIRUS;

EID: 79551691992     PISSN: 0022538X     EISSN: 10985514     Source Type: Journal    
DOI: 10.1128/JVI.01895-10     Document Type: Article

References (38)

1. Abaitua, F., and P. O'Hare. 2008. Identification of a highly conserved, functional nuclear localization signal within the N-terminal region of herpes simplex virus type 1 VP1-2 tegument protein. J. Virol. 82:5234-5244. (Pubitemid 351705232)
2. Abaitua, F., R. N. Souto, H. Browne, T. Daikoku, and P. O'Hare. 2009. Characterization of the herpes simplex virus (HSV)-1 tegument protein VP1-2 during infection with the HSV temperature-sensitive mutant tsB7. J. Gen. Virol. 90:2353-2363.
3. Batterson, W., D. Furlong, and B. Roizman. 1983. Molecular genetics of herpes simplex virus. VIII. Further characterization of a temperature-sensitive mutant defective in release of viral DNA and in other stages of the viral reproductive cycle. J. Virol. 45:397-407.
4. Batterson, W., and B. Roizman. 1983. Characterization of the herpes simplex virion-associated factor responsible for the induction of alpha genes. J. Virol. 46:371-377. (Pubitemid 13130008)
5. Benyesh-Melnick, M., P. A. Schaffer, R. J. Courtney, J. Esparza, and S. Kimura. 1975. Viral gene functions expressed and detected by temperature-sensitive mutants of herpes simplex virus. Cold Spring Harbor Symp. Quant. Biol. 39:731-746.
6. Bottcher, S., et al. 2007. Identification of functional domains within the essential large tegument protein pUL36 of pseudorabies virus. J. Virol. 81:13403-13411. (Pubitemid 350247837)
7. Bottcher, S., et al. 2006. Identification of a 709-amino-acid internal nonessential region within the essential conserved tegument protein (p)UL36 of pseudorabies virus. J. Virol. 80:9910-9915. (Pubitemid 44435667)
8. DeLuca, N. A., M. A. Courtney, and P. A. Schaffer. 1984. Temperature-sensitive mutants in herpes simplex virus type 1 ICP4 permissive for early gene expression. J. Virol. 52:767-776.
9. Desai, P. J. 2000. A null mutation in the UL36 gene of herpes simplex virus type 1 results in accumulation of unenveloped DNA-filled capsids in the cytoplasm of infected cells. J. Virol. 74:11608-116018.
10. Dixon, R. A., and P. A. Schaffer. 1980. Fine-structure mapping and functional analysis of temperature-sensitive mutants in the gene encoding the herpes simplex virus type 1 immediate early protein VP175. J. Virol. 36:189-203.
11. Fuchs, W., B. G. Klupp, H. Granzow, and T. C. Mettenleiter. 2004. Essential function of the pseudorabies virus UL36 gene product is independent of its interaction with the UL37 protein. J. Virol. 78:11879-11889.
12. Granzow, H., B. G. Klupp, and T. C. Mettenleiter. 2005. Entry of pseudorabies virus: an immunogold-labeling study. J. Virol. 79:3200-3205.
13. Jovasevic, V., L. Liang, and B. Roizman. 2008. Proteolytic cleavage of VP1-2 is required for release of herpes simplex virus 1 DNA into the nucleus. J. Virol. 82:3311-3319. (Pubitemid 351429909)
14. Klupp, B. G., W. Fuchs, H. Granzow, R. Nixdorf, and T. C. Mettenleiter. 2002. Pseudorabies virus UL36 tegument protein physically interacts with the UL37 protein. J. Virol. 76:3065-3071.
15. Knipe, D. M., W. Batterson, C. Nosal, B. Roizman, and A. Buchan. 1981. Molecular genetics of herpes simplex virus. VI. Characterization of a temperature-sensitive mutant defective in the expression of all early viral gene products. J. Virol. 38:539-547.
16. Lee, J. I., G. W. Luxton, and G. A. Smith. 2006. Identification of an essential domain in the herpesvirus VP1/2 tegument protein: the carboxy terminus directs incorporation into capsid assemblons. J. Virol. 80:12086-12094.
17. Luxton, G. W., et al. 2005. Targeting of herpesvirus capsid transport in axons is coupled to association with specific sets of tegument proteins. Proc. Natl. Acad. Sci. U. S. A. 102:5832-5837.
18. Luxton, G. W., J. I. Lee, S. Haverlock-Moyns, J. M. Schober, and G. A. Smith. 2006. The pseudorabies virus VP1/2 tegument protein is required for intracellular capsid transport. J. Virol. 80:201-209.
19. Matthews, B. W. 1987. Genetic and structural analysis of the protein stability problem. Biochemistry 26:6885-6888.
20. McGeoch, D. J., et al. 1988. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1. J. Gen. Virol. 69:1531-1574.
21. Michael, K., B. G. Klupp, T. C. Mettenleiter, and A. Karger. 2006. Composition of pseudorabies virus particles lacking tegument protein US3, UL47, or UL49 or envelope glycoprotein E. J. Virol. 80:1332-1339. (Pubitemid 43134090)
22. Mohl, B. S., et al. 2010. Random transposon-mediated mutagenesis of the essential large tegument protein pUL36 of pseudorabies virus. J. Virol. 84:8153-8162.
23. Newcomb, W. W., and J. C. Brown. 14 July 2010. Structure and capsid association of the herpesvirus large tegument protein UL36. J. Virol. doi: 10.1128/JVI.00361-10.
24. Pakula, A. A., and R. T. Sauer. 1989. Genetic analysis of protein stability and function. Annu. Rev. Genet. 23:289-310.
25. Parsell, D. A., and R. T. Sauer. 1989. The structural stability of a protein is an important determinant of its proteolytic susceptibility in Escherichia coli. J. Biol. Chem. 264:7590-7595.
26. Preston, C. M. 1979. Control of herpes simplex virus type 1 mRNA synthesis in cells infected with wild-type virus or the temperature-sensitive mutant tsK. J. Virol. 29:275-284.
27. Preston, V. G. 1981. Fine-structure mapping of herpes simplex virus type 1 temperature-sensitive mutations within the short repeat region of the genome. J. Virol. 39:150-161. (Pubitemid 11069820)
28. Roberts, A. P., et al. 2009. Differing roles of inner tegument proteins pUL36 and pUL37 during entry of herpes simplex virus type 1. J. Virol. 83:105-116.
29. Sadler, J. R., and A. Novick. 1965. The properties of repressor and the kinetics of its action. J. Mol. Biol. 12:305-327.
30. Schaffer, P. A., V. C. Carter, and M. C. Timbury. 1978. Collaborative complementation study of temperature-sensitive mutants of herpes simplex virus types 1 and 2. J. Virol. 27:490-504.
31. Schlieker, C., et al. 2007. Structure of a herpesvirus-encoded cysteine protease reveals a unique class of deubiquitinating enzymes. Mol. Cell 25:677-687. (Pubitemid 46341922)
32. Sturtevant, J. M., M. H. Yu, C. Haase-Pettingell, and J. King. 1989. Thermostability of temperature-sensitive folding mutants of the P22 tailspike protein. J. Biol. Chem. 264:10693-10698.
33. Thomas, G. J., Jr., R. Becka, D. Sargent, M. H. Yu, and J. King. 1990. Conformational stability of P22 tailspike proteins carrying temperature- sensitive folding mutations. Biochemistry 29:4181-4187. (Pubitemid 20145656)
34. Varadarajan, R., H. A. Nagarajaram, and C. Ramakrishnan. 1996. A procedure for the prediction of temperature-sensitive mutants of a globular protein based solely on the amino acid sequence. Proc. Natl. Acad. Sci. U. S. A. 93:13908-13913.
35. Wagner, E. 1985. Individual HSV transcripts: characterisation of specific genes. Herpesviruses 3:45-91.
36. Wolfstein, A., et al. 2006. The inner tegument promotes herpes simplex virus capsid motility along microtubules in vitro. Traffic 7:227-237.
37. Yu, M. H., and J. King. 1988. Surface amino acids as sites of temperature-sensitive folding mutations in the P22 tailspike protein. J. Biol. Chem. 263:1424-1431.
38. Zhang, Y. F., and E. K. Wagner. 1987. The kinetics of expression of individual herpes simplex virus type 1 transcripts. Virus Genes 1:49-60.